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Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L.

Gururaja GM, Mundkinajeddu D, Dethe SM, Sangli GK, Abhilash K, Agarwal A - Pharmacognosy Res (2014 Oct-Dec)

Bottom Line: Active fractions were re-chromatographed to yield individual compounds.However, the result indicates that compounds other than mangiferin may be active in the extract.Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Research Scholar, Manipal University, Manipal, India ; R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.

ABSTRACT

Background: In the earlier studies, methanolic extract of Mangifera indica L leaf was exhibited hypocholesterol activity. However, the bioactive compounds responsible for the same are not reported so far.

Objective: To isolate the bioactive compounds with hypocholesterol activity from the leaf extract using cholesterol esterase inhibition assay which can be used for the standardization of extract.

Materials and methods: The leaf methanolic extract of M. indica (Sindoora variety) was partitioned with ethyl acetate and chromatographed on silica gel to yield twelve fractions and the activity was monitored by using cholesterol esterase inhibition assay. Active fractions were re-chromatographed to yield individual compounds.

Results and discussion: A major compound mangiferin present in the extract was screened along with other varieties of mango leaves for cholesterol esterase inhibition assay. However, the result indicates that compounds other than mangiferin may be active in the extract. Invitro pancreatic cholesterol esterase inhibition assay was used for bioactivity guided fractionation (BAGF) to yield bioactive compound for standardization of extract. Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml.

Conclusion: This study demonstrates that M. indica methanol extract of leaf have significant hypocholesterol activity which is standardized with 3b-taraxerol, a standardized extract for hypocholesterol activity resulted in development of dietary supplement from leaves of Mangifera indica.

No MeSH data available.


Related in: MedlinePlus

Mass spectra of 3-β-taraxerol
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Figure 8: Mass spectra of 3-β-taraxerol

Mentions: The 100% methanol fraction (fraction no XII) from silica column was rechromatographed on silica gel, eluted with increasing polarity using petroleum ether and ethyl acetate followed by increasing percentage of methanol. Each individual collected fraction was concentrated in rotary evaporator to distil the solvents. The 20% methanol in ethyl acetate fraction obtained from above was applied on diaion HP-20 resin column for further purification of compound 2, which was eluted with decreasing polarity using water and acetone. The 20% acetone in water fraction was applied on sephadex LH-20 for further purification of compound eluted using water as solvent. Different fractions were collected and combined based on TLC observation. Final purification was carried out by using the prep HPLC (Kromasil C18 preparative HPLC column [250 mm × 21.2 mm, 5 μm]) was used. The preparative HPLC method was developed using isocratic method containing 15% methanol in water to yield compound 2 (360 mg) which was identified as Iriflophenone-3-β-C-glucoside [Figure 3] based on the spectroscopic data [Figures S5–7].[17]


Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L.

Gururaja GM, Mundkinajeddu D, Dethe SM, Sangli GK, Abhilash K, Agarwal A - Pharmacognosy Res (2014 Oct-Dec)

Mass spectra of 3-β-taraxerol
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660515&req=5

Figure 8: Mass spectra of 3-β-taraxerol
Mentions: The 100% methanol fraction (fraction no XII) from silica column was rechromatographed on silica gel, eluted with increasing polarity using petroleum ether and ethyl acetate followed by increasing percentage of methanol. Each individual collected fraction was concentrated in rotary evaporator to distil the solvents. The 20% methanol in ethyl acetate fraction obtained from above was applied on diaion HP-20 resin column for further purification of compound 2, which was eluted with decreasing polarity using water and acetone. The 20% acetone in water fraction was applied on sephadex LH-20 for further purification of compound eluted using water as solvent. Different fractions were collected and combined based on TLC observation. Final purification was carried out by using the prep HPLC (Kromasil C18 preparative HPLC column [250 mm × 21.2 mm, 5 μm]) was used. The preparative HPLC method was developed using isocratic method containing 15% methanol in water to yield compound 2 (360 mg) which was identified as Iriflophenone-3-β-C-glucoside [Figure 3] based on the spectroscopic data [Figures S5–7].[17]

Bottom Line: Active fractions were re-chromatographed to yield individual compounds.However, the result indicates that compounds other than mangiferin may be active in the extract.Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Research Scholar, Manipal University, Manipal, India ; R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.

ABSTRACT

Background: In the earlier studies, methanolic extract of Mangifera indica L leaf was exhibited hypocholesterol activity. However, the bioactive compounds responsible for the same are not reported so far.

Objective: To isolate the bioactive compounds with hypocholesterol activity from the leaf extract using cholesterol esterase inhibition assay which can be used for the standardization of extract.

Materials and methods: The leaf methanolic extract of M. indica (Sindoora variety) was partitioned with ethyl acetate and chromatographed on silica gel to yield twelve fractions and the activity was monitored by using cholesterol esterase inhibition assay. Active fractions were re-chromatographed to yield individual compounds.

Results and discussion: A major compound mangiferin present in the extract was screened along with other varieties of mango leaves for cholesterol esterase inhibition assay. However, the result indicates that compounds other than mangiferin may be active in the extract. Invitro pancreatic cholesterol esterase inhibition assay was used for bioactivity guided fractionation (BAGF) to yield bioactive compound for standardization of extract. Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml.

Conclusion: This study demonstrates that M. indica methanol extract of leaf have significant hypocholesterol activity which is standardized with 3b-taraxerol, a standardized extract for hypocholesterol activity resulted in development of dietary supplement from leaves of Mangifera indica.

No MeSH data available.


Related in: MedlinePlus