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Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L.

Gururaja GM, Mundkinajeddu D, Dethe SM, Sangli GK, Abhilash K, Agarwal A - Pharmacognosy Res (2014 Oct-Dec)

Bottom Line: Active fractions were re-chromatographed to yield individual compounds.However, the result indicates that compounds other than mangiferin may be active in the extract.Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Research Scholar, Manipal University, Manipal, India ; R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.

ABSTRACT

Background: In the earlier studies, methanolic extract of Mangifera indica L leaf was exhibited hypocholesterol activity. However, the bioactive compounds responsible for the same are not reported so far.

Objective: To isolate the bioactive compounds with hypocholesterol activity from the leaf extract using cholesterol esterase inhibition assay which can be used for the standardization of extract.

Materials and methods: The leaf methanolic extract of M. indica (Sindoora variety) was partitioned with ethyl acetate and chromatographed on silica gel to yield twelve fractions and the activity was monitored by using cholesterol esterase inhibition assay. Active fractions were re-chromatographed to yield individual compounds.

Results and discussion: A major compound mangiferin present in the extract was screened along with other varieties of mango leaves for cholesterol esterase inhibition assay. However, the result indicates that compounds other than mangiferin may be active in the extract. Invitro pancreatic cholesterol esterase inhibition assay was used for bioactivity guided fractionation (BAGF) to yield bioactive compound for standardization of extract. Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml.

Conclusion: This study demonstrates that M. indica methanol extract of leaf have significant hypocholesterol activity which is standardized with 3b-taraxerol, a standardized extract for hypocholesterol activity resulted in development of dietary supplement from leaves of Mangifera indica.

No MeSH data available.


Related in: MedlinePlus

mass spectra of iriflophenone-3-β-C-glucoside
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Figure 6: mass spectra of iriflophenone-3-β-C-glucoside

Mentions: The 15% ethyl acetate in pet ether fraction (FRN IV) enriched with compound 1 was chromatographed on silica gel (300–400 mesh) using flash chromatography (Combi flash) using petroleum ether and chloroform as mobile phase with increasing polarity. The combination of petroleum ether and chloroform would give different polarity combination than the ethyl acetate combination for better separation. (TLC-precoated silica gel 60 F254 plates [Merck]; developing solvent petroleum ether: ethyl acetate [8:2] and the spots are not detected by 254 nm. Hence, it is sprayed with anisaldehyde sulphuric acid reagent followed by heating at 105°C for 5 min). Fractions collected from 20% chloroform to 30% chloroform in petroleum ether was enriched with compound 1, followed by repeated crystallisation by dissolving in hexane to get pure 3 β-taraxerol [Figure 2] characterized based on spectroscopic data [Figures S1–4 ] matched with literature values.[16]


Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L.

Gururaja GM, Mundkinajeddu D, Dethe SM, Sangli GK, Abhilash K, Agarwal A - Pharmacognosy Res (2014 Oct-Dec)

mass spectra of iriflophenone-3-β-C-glucoside
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660515&req=5

Figure 6: mass spectra of iriflophenone-3-β-C-glucoside
Mentions: The 15% ethyl acetate in pet ether fraction (FRN IV) enriched with compound 1 was chromatographed on silica gel (300–400 mesh) using flash chromatography (Combi flash) using petroleum ether and chloroform as mobile phase with increasing polarity. The combination of petroleum ether and chloroform would give different polarity combination than the ethyl acetate combination for better separation. (TLC-precoated silica gel 60 F254 plates [Merck]; developing solvent petroleum ether: ethyl acetate [8:2] and the spots are not detected by 254 nm. Hence, it is sprayed with anisaldehyde sulphuric acid reagent followed by heating at 105°C for 5 min). Fractions collected from 20% chloroform to 30% chloroform in petroleum ether was enriched with compound 1, followed by repeated crystallisation by dissolving in hexane to get pure 3 β-taraxerol [Figure 2] characterized based on spectroscopic data [Figures S1–4 ] matched with literature values.[16]

Bottom Line: Active fractions were re-chromatographed to yield individual compounds.However, the result indicates that compounds other than mangiferin may be active in the extract.Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Research Scholar, Manipal University, Manipal, India ; R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.

ABSTRACT

Background: In the earlier studies, methanolic extract of Mangifera indica L leaf was exhibited hypocholesterol activity. However, the bioactive compounds responsible for the same are not reported so far.

Objective: To isolate the bioactive compounds with hypocholesterol activity from the leaf extract using cholesterol esterase inhibition assay which can be used for the standardization of extract.

Materials and methods: The leaf methanolic extract of M. indica (Sindoora variety) was partitioned with ethyl acetate and chromatographed on silica gel to yield twelve fractions and the activity was monitored by using cholesterol esterase inhibition assay. Active fractions were re-chromatographed to yield individual compounds.

Results and discussion: A major compound mangiferin present in the extract was screened along with other varieties of mango leaves for cholesterol esterase inhibition assay. However, the result indicates that compounds other than mangiferin may be active in the extract. Invitro pancreatic cholesterol esterase inhibition assay was used for bioactivity guided fractionation (BAGF) to yield bioactive compound for standardization of extract. Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml.

Conclusion: This study demonstrates that M. indica methanol extract of leaf have significant hypocholesterol activity which is standardized with 3b-taraxerol, a standardized extract for hypocholesterol activity resulted in development of dietary supplement from leaves of Mangifera indica.

No MeSH data available.


Related in: MedlinePlus