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Chemical constituents of Phragmanthera austroarabica A. G. Mill and J. A. Nyberg with potent antioxidant activity.

Badr JM - Pharmacognosy Res (2014 Oct-Dec)

Bottom Line: Phytochemical investigation of the plant resulted in isolation of 12 compounds.The isolated compounds were identified as chrysophanic acid, emodin, chrysophanic acid-8-O-glucoside, emodin-8-O-glucoside, pectolinarigenin, quercetin, dillenetin-3-O-glucoside, catechin, catechin-4'-O-gallate, methyl gallate, lupeol and ursolic acid.All the isolated phenolic compounds revealed significant free radical scavenging activities when tested using 2,2-diphenyl-1-picrylhydrazyl reagent.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.

ABSTRACT

Background: Phragmanthera austroarabica A.G. Mill. and J. A. Nyberg is a semi parasitic plant belonging to family Loranthaceae. It was collected from Saudi Arabia. It is widely used in folk medicine among the kingdom in treatment of various diseases including diabetes mellitus.

Objective: The total alcoholic extract of P. austroarabica collected from Saudi Arabia was investigated for the chemical structure and prominent biological activity of the main constituents.

Materials and methods: Isolation of the active constituents was performed using different chromatographic techniques including column chromatography packed with silica or sephadex and preparative thin layer chromatography. The structures of the isolated compounds were established based on different spectroscopic data as mass spectrum, one-dimensional and two-dimensional nuclear magnetic resonance (correlation spectroscopy, heteronuclear single quantum coherence, and heteronuclear multiple-bond correlation).

Results: Phytochemical investigation of the plant resulted in isolation of 12 compounds. The isolated compounds were identified as chrysophanic acid, emodin, chrysophanic acid-8-O-glucoside, emodin-8-O-glucoside, pectolinarigenin, quercetin, dillenetin-3-O-glucoside, catechin, catechin-4'-O-gallate, methyl gallate, lupeol and ursolic acid. All the isolated phenolic compounds revealed significant free radical scavenging activities when tested using 2,2-diphenyl-1-picrylhydrazyl reagent.

Conclusion: The antioxidant activities of the isolated compounds can justify the use of P. austroarabica in traditional medicine for treatment of diabetes and verify its possible application as an antihyperglycemic drug.

No MeSH data available.


Related in: MedlinePlus

Free radical scavenging activity of investigated isolated compounds (5 μg/ml) using 2,2-diphenyl-1-picrylhydrazyl reagent
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Figure 2: Free radical scavenging activity of investigated isolated compounds (5 μg/ml) using 2,2-diphenyl-1-picrylhydrazyl reagent

Mentions: Generally, phenolics have been considered as powerful antioxidants and many of them proved to be more potent than vitamins C, E and carotenoids.[1213] All phenolic compounds isolated from P. austroarabica were tested for their free radical scavenging activity using DPPH reagent. Previous investigations on the antioxidant activity of the flavonoid quercetin revealed its powerful effect.[141516] In the present work, quercetin together with other isolated phenolic compounds were examined for their free radical scavenging activity using DPPH reagent at a concentration of 5 μg/ml. The method is based on the reduction of a methanol solution of DPPH in the presence of a hydrogen donating antioxidant due to the formation of the nonradical form DPPH-H.[7] This transformation results in a change in colour from purple to yellow, which was measured spectrophotometrically at 516 nm. The percentage inhibition of the purple colour of the DPPH as a measure of the free radical scavenging activity is shown in Figure 2. Quercetin and catechin-4’-O-gallate revealed the highest activity (89%) followed by methyl gallate (87%). Also; catechin, dillenetin-3-O-glucoside and pectolinarigenin possess high free radical scavenging activities (81, 80 and 77% respectively). Generally, we can notice as illustrated in Figure 2 that all the tested compounds possess antioxidant activities to variable degrees as compared to quercetin the well-known antioxidant flavonoid.[141516]


Chemical constituents of Phragmanthera austroarabica A. G. Mill and J. A. Nyberg with potent antioxidant activity.

Badr JM - Pharmacognosy Res (2014 Oct-Dec)

Free radical scavenging activity of investigated isolated compounds (5 μg/ml) using 2,2-diphenyl-1-picrylhydrazyl reagent
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660512&req=5

Figure 2: Free radical scavenging activity of investigated isolated compounds (5 μg/ml) using 2,2-diphenyl-1-picrylhydrazyl reagent
Mentions: Generally, phenolics have been considered as powerful antioxidants and many of them proved to be more potent than vitamins C, E and carotenoids.[1213] All phenolic compounds isolated from P. austroarabica were tested for their free radical scavenging activity using DPPH reagent. Previous investigations on the antioxidant activity of the flavonoid quercetin revealed its powerful effect.[141516] In the present work, quercetin together with other isolated phenolic compounds were examined for their free radical scavenging activity using DPPH reagent at a concentration of 5 μg/ml. The method is based on the reduction of a methanol solution of DPPH in the presence of a hydrogen donating antioxidant due to the formation of the nonradical form DPPH-H.[7] This transformation results in a change in colour from purple to yellow, which was measured spectrophotometrically at 516 nm. The percentage inhibition of the purple colour of the DPPH as a measure of the free radical scavenging activity is shown in Figure 2. Quercetin and catechin-4’-O-gallate revealed the highest activity (89%) followed by methyl gallate (87%). Also; catechin, dillenetin-3-O-glucoside and pectolinarigenin possess high free radical scavenging activities (81, 80 and 77% respectively). Generally, we can notice as illustrated in Figure 2 that all the tested compounds possess antioxidant activities to variable degrees as compared to quercetin the well-known antioxidant flavonoid.[141516]

Bottom Line: Phytochemical investigation of the plant resulted in isolation of 12 compounds.The isolated compounds were identified as chrysophanic acid, emodin, chrysophanic acid-8-O-glucoside, emodin-8-O-glucoside, pectolinarigenin, quercetin, dillenetin-3-O-glucoside, catechin, catechin-4'-O-gallate, methyl gallate, lupeol and ursolic acid.All the isolated phenolic compounds revealed significant free radical scavenging activities when tested using 2,2-diphenyl-1-picrylhydrazyl reagent.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.

ABSTRACT

Background: Phragmanthera austroarabica A.G. Mill. and J. A. Nyberg is a semi parasitic plant belonging to family Loranthaceae. It was collected from Saudi Arabia. It is widely used in folk medicine among the kingdom in treatment of various diseases including diabetes mellitus.

Objective: The total alcoholic extract of P. austroarabica collected from Saudi Arabia was investigated for the chemical structure and prominent biological activity of the main constituents.

Materials and methods: Isolation of the active constituents was performed using different chromatographic techniques including column chromatography packed with silica or sephadex and preparative thin layer chromatography. The structures of the isolated compounds were established based on different spectroscopic data as mass spectrum, one-dimensional and two-dimensional nuclear magnetic resonance (correlation spectroscopy, heteronuclear single quantum coherence, and heteronuclear multiple-bond correlation).

Results: Phytochemical investigation of the plant resulted in isolation of 12 compounds. The isolated compounds were identified as chrysophanic acid, emodin, chrysophanic acid-8-O-glucoside, emodin-8-O-glucoside, pectolinarigenin, quercetin, dillenetin-3-O-glucoside, catechin, catechin-4'-O-gallate, methyl gallate, lupeol and ursolic acid. All the isolated phenolic compounds revealed significant free radical scavenging activities when tested using 2,2-diphenyl-1-picrylhydrazyl reagent.

Conclusion: The antioxidant activities of the isolated compounds can justify the use of P. austroarabica in traditional medicine for treatment of diabetes and verify its possible application as an antihyperglycemic drug.

No MeSH data available.


Related in: MedlinePlus