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Alpha-amylase inhibitory activity and sterol composition of the marine algae, Sargassum glaucescens.

Payghami N, Jamili S, Rustaiyan A, Saeidnia S, Nikan M, Gohari AR - Pharmacognosy Res (2014 Oct-Dec)

Bottom Line: In vitro alpha-amylase inhibitory test was performed on the methanolic extract and the results revealed a potent inhibition (IC50 = 8.9 ± 2.4 mg/mL) of the enzyme compared to acarbose as a positive control.Various biological activities and distribution of sterols in Sargassum genus have been critically reviewed here.The results concluded that these algae are a good candidate for further anti-diabetic investigations in animals and human.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Sargassum species (phaeophyceae) are economically important brown algae in southern parts of Iran. Sargassum is mainly harvested as a row material in alginate production industries and is a source of plant foods or plant bio-stimulants even a component of animal foods.

Objective: In this study, Sargassum glaucescens, collected from the seashore of Chabahar, was employed for phytochemical and biological evaluations.

Materials and methods: For that purpose, the dried algae was extracted by methanol and subjected to different chromatographic separation methods.

Results: Six sterols, fucosterol (1), 24(S)-hydroxy-24-vinylcholesterol (2), 24(R)-hydroxy-24-vinylcholesterol (3), stigmasterol (4), β-sitosterol (5) and cholesterol (6) were identified by spectroscopic methods including (1)H-NMR, (13)C-NMR and mass spectroscopy. In vitro alpha-amylase inhibitory test was performed on the methanolic extract and the results revealed a potent inhibition (IC50 = 8.9 ± 2.4 mg/mL) of the enzyme compared to acarbose as a positive control.

Conclusion: Various biological activities and distribution of sterols in Sargassum genus have been critically reviewed here. The results concluded that these algae are a good candidate for further anti-diabetic investigations in animals and human.

No MeSH data available.


Related in: MedlinePlus

Chemical structures of isolated sterols from Sargassum glaucescens
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Figure 1: Chemical structures of isolated sterols from Sargassum glaucescens

Mentions: Methanolic extract of marine algae, S. glaucescens, was used for the separation of sterols. Isolation and purification of the main compounds were carried out on silica gel and sephadex LH20 CC to obtain six pure compounds. Structural elucidation of these compounds was based on the data obtained from 1H-NMR, 13C-NMR studies. Separated compounds from S. glaucescens were identified as fucosterol (1),[31] a mixture of 24(S)-hydroxy-24-vinylcholesterol (2) and 24(R)-hydroxy-24-vinylcholesterol (3),[15] stigmasterol (4),[3233] β-sitosterol (5)[3435] and cholesterol (6)[36] compared to the spectral data reported in the literatures [Figure 1]. 13C NMR data of sterols isolated from S. glaucescens are summarized in Table 2.


Alpha-amylase inhibitory activity and sterol composition of the marine algae, Sargassum glaucescens.

Payghami N, Jamili S, Rustaiyan A, Saeidnia S, Nikan M, Gohari AR - Pharmacognosy Res (2014 Oct-Dec)

Chemical structures of isolated sterols from Sargassum glaucescens
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660509&req=5

Figure 1: Chemical structures of isolated sterols from Sargassum glaucescens
Mentions: Methanolic extract of marine algae, S. glaucescens, was used for the separation of sterols. Isolation and purification of the main compounds were carried out on silica gel and sephadex LH20 CC to obtain six pure compounds. Structural elucidation of these compounds was based on the data obtained from 1H-NMR, 13C-NMR studies. Separated compounds from S. glaucescens were identified as fucosterol (1),[31] a mixture of 24(S)-hydroxy-24-vinylcholesterol (2) and 24(R)-hydroxy-24-vinylcholesterol (3),[15] stigmasterol (4),[3233] β-sitosterol (5)[3435] and cholesterol (6)[36] compared to the spectral data reported in the literatures [Figure 1]. 13C NMR data of sterols isolated from S. glaucescens are summarized in Table 2.

Bottom Line: In vitro alpha-amylase inhibitory test was performed on the methanolic extract and the results revealed a potent inhibition (IC50 = 8.9 ± 2.4 mg/mL) of the enzyme compared to acarbose as a positive control.Various biological activities and distribution of sterols in Sargassum genus have been critically reviewed here.The results concluded that these algae are a good candidate for further anti-diabetic investigations in animals and human.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Sargassum species (phaeophyceae) are economically important brown algae in southern parts of Iran. Sargassum is mainly harvested as a row material in alginate production industries and is a source of plant foods or plant bio-stimulants even a component of animal foods.

Objective: In this study, Sargassum glaucescens, collected from the seashore of Chabahar, was employed for phytochemical and biological evaluations.

Materials and methods: For that purpose, the dried algae was extracted by methanol and subjected to different chromatographic separation methods.

Results: Six sterols, fucosterol (1), 24(S)-hydroxy-24-vinylcholesterol (2), 24(R)-hydroxy-24-vinylcholesterol (3), stigmasterol (4), β-sitosterol (5) and cholesterol (6) were identified by spectroscopic methods including (1)H-NMR, (13)C-NMR and mass spectroscopy. In vitro alpha-amylase inhibitory test was performed on the methanolic extract and the results revealed a potent inhibition (IC50 = 8.9 ± 2.4 mg/mL) of the enzyme compared to acarbose as a positive control.

Conclusion: Various biological activities and distribution of sterols in Sargassum genus have been critically reviewed here. The results concluded that these algae are a good candidate for further anti-diabetic investigations in animals and human.

No MeSH data available.


Related in: MedlinePlus