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IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES.

Corral MA, Paula FM, Gottardi M, Meisel DM, Castilho VL, Gonçalves EM, Chieffi PP, Gryschek RC - Rev. Inst. Med. Trop. Sao Paulo (2015 Sep-Oct)

Bottom Line: Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively.Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively.In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis.

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Related in: MedlinePlus

- ROC curve indicating the optimum point of reaction (cut-off), sensitivity (Se), specificity (Sp), diagnostic efficiency (DE), kappa index (k), likelihood ratio (LR), and area under curve (AUC) for IgG detection in serum samples using soluble (SSF, TSF, and ASF) and membrane (SMF, TMF, and AMF) fractions from Strongyloides venezuelensis parasitic females.
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f02: - ROC curve indicating the optimum point of reaction (cut-off), sensitivity (Se), specificity (Sp), diagnostic efficiency (DE), kappa index (k), likelihood ratio (LR), and area under curve (AUC) for IgG detection in serum samples using soluble (SSF, TSF, and ASF) and membrane (SMF, TMF, and AMF) fractions from Strongyloides venezuelensis parasitic females.

Mentions: The diagnostic parameters and efficiency of ELISA for the six antigenic fractions are shown in Fig. 2. Among the soluble fractions, SSF had the highest value of sensitivity and specificity (85% and 93.1%, respectively). The value of sensitivity and specificity for the membrane fraction SMF were also high, at 80% and 95.8%, respectively. The k values ranged from 0.619 to 0.753 for the soluble fractions, and 0.630 to 0.772 for the membrane fractions. Of the six fractions tested, those obtained using PBS provided optimal diagnostic parameters. An LR greater than 10 indicates high performance with respect to the detection of specific IgG antibodies in patients with strongyloidiasis7. In this study, the antigenic fractions obtained with PBS had high LRs (12.2-19.2), indicating that these preparations had high ability to discriminate between strongyloidiasis patients and controls.


IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES.

Corral MA, Paula FM, Gottardi M, Meisel DM, Castilho VL, Gonçalves EM, Chieffi PP, Gryschek RC - Rev. Inst. Med. Trop. Sao Paulo (2015 Sep-Oct)

- ROC curve indicating the optimum point of reaction (cut-off), sensitivity (Se), specificity (Sp), diagnostic efficiency (DE), kappa index (k), likelihood ratio (LR), and area under curve (AUC) for IgG detection in serum samples using soluble (SSF, TSF, and ASF) and membrane (SMF, TMF, and AMF) fractions from Strongyloides venezuelensis parasitic females.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660453&req=5

f02: - ROC curve indicating the optimum point of reaction (cut-off), sensitivity (Se), specificity (Sp), diagnostic efficiency (DE), kappa index (k), likelihood ratio (LR), and area under curve (AUC) for IgG detection in serum samples using soluble (SSF, TSF, and ASF) and membrane (SMF, TMF, and AMF) fractions from Strongyloides venezuelensis parasitic females.
Mentions: The diagnostic parameters and efficiency of ELISA for the six antigenic fractions are shown in Fig. 2. Among the soluble fractions, SSF had the highest value of sensitivity and specificity (85% and 93.1%, respectively). The value of sensitivity and specificity for the membrane fraction SMF were also high, at 80% and 95.8%, respectively. The k values ranged from 0.619 to 0.753 for the soluble fractions, and 0.630 to 0.772 for the membrane fractions. Of the six fractions tested, those obtained using PBS provided optimal diagnostic parameters. An LR greater than 10 indicates high performance with respect to the detection of specific IgG antibodies in patients with strongyloidiasis7. In this study, the antigenic fractions obtained with PBS had high LRs (12.2-19.2), indicating that these preparations had high ability to discriminate between strongyloidiasis patients and controls.

Bottom Line: Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively.Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively.In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis.

Show MeSH
Related in: MedlinePlus