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Harpin Hpa1 Interacts with Aquaporin PIP1;4 to Promote the Substrate Transport and Photosynthesis in Arabidopsis.

Li L, Wang H, Gago J, Cui H, Qian Z, Kodama N, Ji H, Tian S, Shen D, Chen Y, Sun F, Xia Z, Ye Q, Sun W, Flexas J, Dong H - Sci Rep (2015)

Bottom Line: In particular, AtPIP1;4 mediates CO2 transport with a substantial contribute to photosynthesis and further increases this function upon interacting with Hpa1 at the plasma membrane.As a result, leaf photosynthesis rates are increased and the plant growth is enhanced in contrast to the normal process without Hpa1-AtPIP1;4 interaction.Our findings demonstrate the first case that plant sensing of a bacterial harpin protein is connected with photosynthetic physiology to regulate plant growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
Harpin proteins produced by plant-pathogenic Gram-negative bacteria are the venerable player in regulating bacterial virulence and inducing plant growth and defenses. A major gap in these effects is plant sensing linked to cellular responses, and plant sensor for harpin Hpa1 from rice bacterial blight pathogen points to plasma membrane intrinsic protein (PIP). Here we show that Arabidopsis AtPIP1;4 is a plasma membrane sensor of Hpa1 and plays a dual role in plasma membrane permeability of CO2 and H2O. In particular, AtPIP1;4 mediates CO2 transport with a substantial contribute to photosynthesis and further increases this function upon interacting with Hpa1 at the plasma membrane. As a result, leaf photosynthesis rates are increased and the plant growth is enhanced in contrast to the normal process without Hpa1-AtPIP1;4 interaction. Our findings demonstrate the first case that plant sensing of a bacterial harpin protein is connected with photosynthetic physiology to regulate plant growth.

No MeSH data available.


Related in: MedlinePlus

Arabidopsis cell hydraulic conductivity.Data shown are means ± SEMs (n = 20 cells). Different letters on error bars indicate significant (P < 0.05) differences.
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f6: Arabidopsis cell hydraulic conductivity.Data shown are means ± SEMs (n = 20 cells). Different letters on error bars indicate significant (P < 0.05) differences.

Mentions: In addition to mediating mesophyll CO2 conductance, AtPIP1;4 also facilitates H2O transport across PMs of living cells. We found that de novo expression of AtPIP1;4 was able to increase osmotic water permeability (Pf) of African clawed frog Xenopus laevis oocytes. Values of Pf were determined to be 22.35 ± 2.85 and 17.33 ± 2.85 μm/s in oocytes following injection with cRNAs of AtPIP1;4:His and His used as a control, respectively. The difference in Pf values between AtPIP1;4:His and His were statistically significant (P < 0.01). This result was in agreement with cell pressure probe measurements39 performed on intact plants of Arabidopsis. In cell pressure probing assays, atpip1;4-3 and WT plants displayed significant differences (P < 0.05) between each other in parameters of water relations except for cell volume and cell surface area (Supplementary Table 2). In particular, root cortical cell hydraulic conductivity (Lprc) and leaf cell hydraulic conductivity (Lplc) were higher in WT than in atpip1;4-3, with a significant difference (P < 0.05) in Lplc (Fig. 6). Based on the differences between WT and atpip1;4-3 plants, AtPIP1;4 contributed to 16% (0.72 vs. 0.62) of Lprc and 37% (1.67 vs. 1.22) of Lplc (Supplementary Table 2). Evidently, AtPIP1;4 plays a role in H2O transport across PMs of Arabidopsis cells.


Harpin Hpa1 Interacts with Aquaporin PIP1;4 to Promote the Substrate Transport and Photosynthesis in Arabidopsis.

Li L, Wang H, Gago J, Cui H, Qian Z, Kodama N, Ji H, Tian S, Shen D, Chen Y, Sun F, Xia Z, Ye Q, Sun W, Flexas J, Dong H - Sci Rep (2015)

Arabidopsis cell hydraulic conductivity.Data shown are means ± SEMs (n = 20 cells). Different letters on error bars indicate significant (P < 0.05) differences.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660436&req=5

f6: Arabidopsis cell hydraulic conductivity.Data shown are means ± SEMs (n = 20 cells). Different letters on error bars indicate significant (P < 0.05) differences.
Mentions: In addition to mediating mesophyll CO2 conductance, AtPIP1;4 also facilitates H2O transport across PMs of living cells. We found that de novo expression of AtPIP1;4 was able to increase osmotic water permeability (Pf) of African clawed frog Xenopus laevis oocytes. Values of Pf were determined to be 22.35 ± 2.85 and 17.33 ± 2.85 μm/s in oocytes following injection with cRNAs of AtPIP1;4:His and His used as a control, respectively. The difference in Pf values between AtPIP1;4:His and His were statistically significant (P < 0.01). This result was in agreement with cell pressure probe measurements39 performed on intact plants of Arabidopsis. In cell pressure probing assays, atpip1;4-3 and WT plants displayed significant differences (P < 0.05) between each other in parameters of water relations except for cell volume and cell surface area (Supplementary Table 2). In particular, root cortical cell hydraulic conductivity (Lprc) and leaf cell hydraulic conductivity (Lplc) were higher in WT than in atpip1;4-3, with a significant difference (P < 0.05) in Lplc (Fig. 6). Based on the differences between WT and atpip1;4-3 plants, AtPIP1;4 contributed to 16% (0.72 vs. 0.62) of Lprc and 37% (1.67 vs. 1.22) of Lplc (Supplementary Table 2). Evidently, AtPIP1;4 plays a role in H2O transport across PMs of Arabidopsis cells.

Bottom Line: In particular, AtPIP1;4 mediates CO2 transport with a substantial contribute to photosynthesis and further increases this function upon interacting with Hpa1 at the plasma membrane.As a result, leaf photosynthesis rates are increased and the plant growth is enhanced in contrast to the normal process without Hpa1-AtPIP1;4 interaction.Our findings demonstrate the first case that plant sensing of a bacterial harpin protein is connected with photosynthetic physiology to regulate plant growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
Harpin proteins produced by plant-pathogenic Gram-negative bacteria are the venerable player in regulating bacterial virulence and inducing plant growth and defenses. A major gap in these effects is plant sensing linked to cellular responses, and plant sensor for harpin Hpa1 from rice bacterial blight pathogen points to plasma membrane intrinsic protein (PIP). Here we show that Arabidopsis AtPIP1;4 is a plasma membrane sensor of Hpa1 and plays a dual role in plasma membrane permeability of CO2 and H2O. In particular, AtPIP1;4 mediates CO2 transport with a substantial contribute to photosynthesis and further increases this function upon interacting with Hpa1 at the plasma membrane. As a result, leaf photosynthesis rates are increased and the plant growth is enhanced in contrast to the normal process without Hpa1-AtPIP1;4 interaction. Our findings demonstrate the first case that plant sensing of a bacterial harpin protein is connected with photosynthetic physiology to regulate plant growth.

No MeSH data available.


Related in: MedlinePlus