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Nucleoside phosphorylation by the mineral schreibersite.

Gull M, Mojica MA, Fernández FM, Gaul DA, Orlando TM, Liotta CL, Pasek MA - Sci Rep (2015)

Bottom Line: Phosphorylation of the nucleosides adenosine and uridine by the simple mixing and mild heating of aqueous solutions of the organic compounds with synthetic analogs of the meteoritic mineral schreibersite, (Fe,Ni)3P under slightly basic conditions (pH ~9) is reported.These results suggest a potential role for meteoritic phosphorus in the origin and development of early life.

View Article: PubMed Central - PubMed

Affiliation: School of Geoscience, University of South Florida, 4202 E Fowler Ave, Tampa FL 33620.

ABSTRACT
Phosphorylation of the nucleosides adenosine and uridine by the simple mixing and mild heating of aqueous solutions of the organic compounds with synthetic analogs of the meteoritic mineral schreibersite, (Fe,Ni)3P under slightly basic conditions (pH ~9) is reported. These results suggest a potential role for meteoritic phosphorus in the origin and development of early life.

No MeSH data available.


Related in: MedlinePlus

31P NMR spectrum of reaction mixture resulting from mixingadenosine with Fe3P (equivalent to sample Ad3, but with an additionalsubstrate, FeS; FeS was not found to influence these results).Peaks are identified based on peak position vs. standards, and fromJ-coupling constants (~5 Hz for 3 bondH-P interactions). The doublets are CH-O-P interactions, and the triplet isa CH2-O-P interaction. Species identification was confirmed byspiking with an adenosine monophosphate standard, causing the triplet toincrease in signal strength. The peak at 5.1 corresponds to phosphite(HPO32−), and the peak at 5.6 isorthophosphate (HPO42−). Bothcompounds are common products during phosphide corrosion14,and a majority of the total dissolved P is in these two species.
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f3: 31P NMR spectrum of reaction mixture resulting from mixingadenosine with Fe3P (equivalent to sample Ad3, but with an additionalsubstrate, FeS; FeS was not found to influence these results).Peaks are identified based on peak position vs. standards, and fromJ-coupling constants (~5 Hz for 3 bondH-P interactions). The doublets are CH-O-P interactions, and the triplet isa CH2-O-P interaction. Species identification was confirmed byspiking with an adenosine monophosphate standard, causing the triplet toincrease in signal strength. The peak at 5.1 corresponds to phosphite(HPO32−), and the peak at 5.6 isorthophosphate (HPO42−). Bothcompounds are common products during phosphide corrosion14,and a majority of the total dissolved P is in these two species.

Mentions: Nucleosides were successfully phosphorylated by Fe3P, and Fe2NiP(Figs 1 and 2 and Table1). Identity of reaction products was confirmed by accurate massmeasurements and matching of chromatographic elution time and MS/MS fragmentationspectra against standards. Nucleotides were produced at concentrations ranging between 1to 6% of total dissolved P in those solutions where they were detected (Table 1); and in the case of adenosine phosphorylation by Fe3Pin the presence of urea and MgSO4, this result was further confirmed by NMR(Fig. 3). Although the yields of phosphorylated products werelow, it appeared that the pH of the aqueous phase was a dominant variable in thephosphorylation outcomes. All experiments indicated that a basic pH was required for theproduction of phosphorylated products. Phosphorylation yields appeared to be somewhatproportional to the amount of phosphorus dissolved in solution and potentiallyindependent of the presence of nickel in the starting schreibersite simulant.


Nucleoside phosphorylation by the mineral schreibersite.

Gull M, Mojica MA, Fernández FM, Gaul DA, Orlando TM, Liotta CL, Pasek MA - Sci Rep (2015)

31P NMR spectrum of reaction mixture resulting from mixingadenosine with Fe3P (equivalent to sample Ad3, but with an additionalsubstrate, FeS; FeS was not found to influence these results).Peaks are identified based on peak position vs. standards, and fromJ-coupling constants (~5 Hz for 3 bondH-P interactions). The doublets are CH-O-P interactions, and the triplet isa CH2-O-P interaction. Species identification was confirmed byspiking with an adenosine monophosphate standard, causing the triplet toincrease in signal strength. The peak at 5.1 corresponds to phosphite(HPO32−), and the peak at 5.6 isorthophosphate (HPO42−). Bothcompounds are common products during phosphide corrosion14,and a majority of the total dissolved P is in these two species.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660433&req=5

f3: 31P NMR spectrum of reaction mixture resulting from mixingadenosine with Fe3P (equivalent to sample Ad3, but with an additionalsubstrate, FeS; FeS was not found to influence these results).Peaks are identified based on peak position vs. standards, and fromJ-coupling constants (~5 Hz for 3 bondH-P interactions). The doublets are CH-O-P interactions, and the triplet isa CH2-O-P interaction. Species identification was confirmed byspiking with an adenosine monophosphate standard, causing the triplet toincrease in signal strength. The peak at 5.1 corresponds to phosphite(HPO32−), and the peak at 5.6 isorthophosphate (HPO42−). Bothcompounds are common products during phosphide corrosion14,and a majority of the total dissolved P is in these two species.
Mentions: Nucleosides were successfully phosphorylated by Fe3P, and Fe2NiP(Figs 1 and 2 and Table1). Identity of reaction products was confirmed by accurate massmeasurements and matching of chromatographic elution time and MS/MS fragmentationspectra against standards. Nucleotides were produced at concentrations ranging between 1to 6% of total dissolved P in those solutions where they were detected (Table 1); and in the case of adenosine phosphorylation by Fe3Pin the presence of urea and MgSO4, this result was further confirmed by NMR(Fig. 3). Although the yields of phosphorylated products werelow, it appeared that the pH of the aqueous phase was a dominant variable in thephosphorylation outcomes. All experiments indicated that a basic pH was required for theproduction of phosphorylated products. Phosphorylation yields appeared to be somewhatproportional to the amount of phosphorus dissolved in solution and potentiallyindependent of the presence of nickel in the starting schreibersite simulant.

Bottom Line: Phosphorylation of the nucleosides adenosine and uridine by the simple mixing and mild heating of aqueous solutions of the organic compounds with synthetic analogs of the meteoritic mineral schreibersite, (Fe,Ni)3P under slightly basic conditions (pH ~9) is reported.These results suggest a potential role for meteoritic phosphorus in the origin and development of early life.

View Article: PubMed Central - PubMed

Affiliation: School of Geoscience, University of South Florida, 4202 E Fowler Ave, Tampa FL 33620.

ABSTRACT
Phosphorylation of the nucleosides adenosine and uridine by the simple mixing and mild heating of aqueous solutions of the organic compounds with synthetic analogs of the meteoritic mineral schreibersite, (Fe,Ni)3P under slightly basic conditions (pH ~9) is reported. These results suggest a potential role for meteoritic phosphorus in the origin and development of early life.

No MeSH data available.


Related in: MedlinePlus