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Nucleoside phosphorylation by the mineral schreibersite.

Gull M, Mojica MA, Fernández FM, Gaul DA, Orlando TM, Liotta CL, Pasek MA - Sci Rep (2015)

Bottom Line: Phosphorylation of the nucleosides adenosine and uridine by the simple mixing and mild heating of aqueous solutions of the organic compounds with synthetic analogs of the meteoritic mineral schreibersite, (Fe,Ni)3P under slightly basic conditions (pH ~9) is reported.These results suggest a potential role for meteoritic phosphorus in the origin and development of early life.

View Article: PubMed Central - PubMed

Affiliation: School of Geoscience, University of South Florida, 4202 E Fowler Ave, Tampa FL 33620.

ABSTRACT
Phosphorylation of the nucleosides adenosine and uridine by the simple mixing and mild heating of aqueous solutions of the organic compounds with synthetic analogs of the meteoritic mineral schreibersite, (Fe,Ni)3P under slightly basic conditions (pH ~9) is reported. These results suggest a potential role for meteoritic phosphorus in the origin and development of early life.

No MeSH data available.


Related in: MedlinePlus

Tandem MS (MS/MS) fragmentation spectra for 5′, 3′,and 2′ adenosine monophosphate standards and correspondingretention-time matched chromatographic peaks in sample Ad4.A collision energy of 15 eV was used. The precursor ion selectedin all cases was m/z 346.05. Fragmentation spectra for other reactionmixtures may be found in the SI. (a) 50 μM5′AMP standard(R.T. = 6.06 min); (b)50 μM 3′AMP standard(R.T. = 6.75 min); (c)50 μM 2′ AMP standard(R.T. = 7.09 min); (d) reactionAd4 5′AMP (R.T. = 6.11 min);(e) reaction Ad4 3′AMP(R.T. = 6.80 min); (f) reactionAd4 2′AMP(R.T. = 7.14 min).
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f2: Tandem MS (MS/MS) fragmentation spectra for 5′, 3′,and 2′ adenosine monophosphate standards and correspondingretention-time matched chromatographic peaks in sample Ad4.A collision energy of 15 eV was used. The precursor ion selectedin all cases was m/z 346.05. Fragmentation spectra for other reactionmixtures may be found in the SI. (a) 50 μM5′AMP standard(R.T. = 6.06 min); (b)50 μM 3′AMP standard(R.T. = 6.75 min); (c)50 μM 2′ AMP standard(R.T. = 7.09 min); (d) reactionAd4 5′AMP (R.T. = 6.11 min);(e) reaction Ad4 3′AMP(R.T. = 6.80 min); (f) reactionAd4 2′AMP(R.T. = 7.14 min).

Mentions: Nucleosides were successfully phosphorylated by Fe3P, and Fe2NiP(Figs 1 and 2 and Table1). Identity of reaction products was confirmed by accurate massmeasurements and matching of chromatographic elution time and MS/MS fragmentationspectra against standards. Nucleotides were produced at concentrations ranging between 1to 6% of total dissolved P in those solutions where they were detected (Table 1); and in the case of adenosine phosphorylation by Fe3Pin the presence of urea and MgSO4, this result was further confirmed by NMR(Fig. 3). Although the yields of phosphorylated products werelow, it appeared that the pH of the aqueous phase was a dominant variable in thephosphorylation outcomes. All experiments indicated that a basic pH was required for theproduction of phosphorylated products. Phosphorylation yields appeared to be somewhatproportional to the amount of phosphorus dissolved in solution and potentiallyindependent of the presence of nickel in the starting schreibersite simulant.


Nucleoside phosphorylation by the mineral schreibersite.

Gull M, Mojica MA, Fernández FM, Gaul DA, Orlando TM, Liotta CL, Pasek MA - Sci Rep (2015)

Tandem MS (MS/MS) fragmentation spectra for 5′, 3′,and 2′ adenosine monophosphate standards and correspondingretention-time matched chromatographic peaks in sample Ad4.A collision energy of 15 eV was used. The precursor ion selectedin all cases was m/z 346.05. Fragmentation spectra for other reactionmixtures may be found in the SI. (a) 50 μM5′AMP standard(R.T. = 6.06 min); (b)50 μM 3′AMP standard(R.T. = 6.75 min); (c)50 μM 2′ AMP standard(R.T. = 7.09 min); (d) reactionAd4 5′AMP (R.T. = 6.11 min);(e) reaction Ad4 3′AMP(R.T. = 6.80 min); (f) reactionAd4 2′AMP(R.T. = 7.14 min).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660433&req=5

f2: Tandem MS (MS/MS) fragmentation spectra for 5′, 3′,and 2′ adenosine monophosphate standards and correspondingretention-time matched chromatographic peaks in sample Ad4.A collision energy of 15 eV was used. The precursor ion selectedin all cases was m/z 346.05. Fragmentation spectra for other reactionmixtures may be found in the SI. (a) 50 μM5′AMP standard(R.T. = 6.06 min); (b)50 μM 3′AMP standard(R.T. = 6.75 min); (c)50 μM 2′ AMP standard(R.T. = 7.09 min); (d) reactionAd4 5′AMP (R.T. = 6.11 min);(e) reaction Ad4 3′AMP(R.T. = 6.80 min); (f) reactionAd4 2′AMP(R.T. = 7.14 min).
Mentions: Nucleosides were successfully phosphorylated by Fe3P, and Fe2NiP(Figs 1 and 2 and Table1). Identity of reaction products was confirmed by accurate massmeasurements and matching of chromatographic elution time and MS/MS fragmentationspectra against standards. Nucleotides were produced at concentrations ranging between 1to 6% of total dissolved P in those solutions where they were detected (Table 1); and in the case of adenosine phosphorylation by Fe3Pin the presence of urea and MgSO4, this result was further confirmed by NMR(Fig. 3). Although the yields of phosphorylated products werelow, it appeared that the pH of the aqueous phase was a dominant variable in thephosphorylation outcomes. All experiments indicated that a basic pH was required for theproduction of phosphorylated products. Phosphorylation yields appeared to be somewhatproportional to the amount of phosphorus dissolved in solution and potentiallyindependent of the presence of nickel in the starting schreibersite simulant.

Bottom Line: Phosphorylation of the nucleosides adenosine and uridine by the simple mixing and mild heating of aqueous solutions of the organic compounds with synthetic analogs of the meteoritic mineral schreibersite, (Fe,Ni)3P under slightly basic conditions (pH ~9) is reported.These results suggest a potential role for meteoritic phosphorus in the origin and development of early life.

View Article: PubMed Central - PubMed

Affiliation: School of Geoscience, University of South Florida, 4202 E Fowler Ave, Tampa FL 33620.

ABSTRACT
Phosphorylation of the nucleosides adenosine and uridine by the simple mixing and mild heating of aqueous solutions of the organic compounds with synthetic analogs of the meteoritic mineral schreibersite, (Fe,Ni)3P under slightly basic conditions (pH ~9) is reported. These results suggest a potential role for meteoritic phosphorus in the origin and development of early life.

No MeSH data available.


Related in: MedlinePlus