Limits...
Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of Francisella tularensis.

Hua F, Zhang P, Zhang F, Zhao Y, Li C, Sun C, Wang X, Yang R, Wang C, Yu A, Zhou L - Sci Rep (2015)

Bottom Line: Except Shigella dysenteriae, UPT-LF showed excellent specificity to four strains that are also potential biowarfare/bioterrorism agents and 13 food-borne pathogenic strains.For practical utilization, the tolerance limits for seven powders and eight viscera were determined, and operation errors of liquid measurement demonstrated a minor influence on the strip.Ftu-UPT-LF is a candidate POCT method because of its excellent sensitivity, specificity, and stability in complex samples, as well as low operation error.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P.R. China.

ABSTRACT
Francisella tularensis is a potential biowarfare/bioterrorism agent and zoonotic pathogen that causes tularemia; thus, surveillance of F. tularensis and first-level emergency response using point-of-care testing (POCT) are essential. The UPT-LF POCT assay was established to quantitatively detect F. tularensis within 15 min, and the sensitivity of the assay was 10(4) CFU · mL(-1) (100 CFU/test). The linear quantitative range covered five orders of magnitude, and the coefficients of variation were less than 10%. Except Shigella dysenteriae, UPT-LF showed excellent specificity to four strains that are also potential biowarfare/bioterrorism agents and 13 food-borne pathogenic strains. Samples with pH 2-13, high ion strengths (≥ 2 mol · L(-1) solution of KCl and NaCl), high viscosities (≤ 50 mg · mL(-1) PEG20000 or ≥ 20% glycerol), and high concentrations of biomacromolecules (≥ 400 mg · mL(-1) bovine serum albumin or ≥ 80 mg · mL(-1) casein) showed little influence on the assay. For practical utilization, the tolerance limits for seven powders and eight viscera were determined, and operation errors of liquid measurement demonstrated a minor influence on the strip. Ftu-UPT-LF is a candidate POCT method because of its excellent sensitivity, specificity, and stability in complex samples, as well as low operation error.

No MeSH data available.


Related in: MedlinePlus

Tolerance of Ftu-UPT-LF to the real sample.Ftu-UPT-LF maintained sensitivity and specificity (*) and even improved thesensitivity by 10-fold (**) under the influence of powders and viscera ofgradient concentrations.
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f4: Tolerance of Ftu-UPT-LF to the real sample.Ftu-UPT-LF maintained sensitivity and specificity (*) and even improved thesensitivity by 10-fold (**) under the influence of powders and viscera ofgradient concentrations.

Mentions: The “white” powders in terrorist attacks (includingflour, fruit juice, gourmet powder, milk powder, putty powder, and sucrose),environmental material (soil), and viscera present in the surveillance center(including fresh and decomposed states) were prepared into variousconcentrations of solutions or homogenates to evaluate the tolerance ofFtu-UPT-LF. As Fig. 4 shows, Ftu-UPT-LF could tolerate 50,100, and200 mg · mL−1flour, fruit juice, gourmet powder, putty powder, and sucrose. With increasingconcentration, milk powder decreased the T/C ratios and destroyed thesensitivity of Ftu-UPT-LF at100 mg · mL−1.By contrast, soil increased the T/C ratios, resulting in a 10-fold improvementin sensitivity at50 mg · mL−1,and the specificity was destroyed at200 mg · mL−1.Ftu-UPT-LF showed excellent tolerance to all viscera with concentrations rangingfrom100 mg · mL−1to400 mg · mL−1.


Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of Francisella tularensis.

Hua F, Zhang P, Zhang F, Zhao Y, Li C, Sun C, Wang X, Yang R, Wang C, Yu A, Zhou L - Sci Rep (2015)

Tolerance of Ftu-UPT-LF to the real sample.Ftu-UPT-LF maintained sensitivity and specificity (*) and even improved thesensitivity by 10-fold (**) under the influence of powders and viscera ofgradient concentrations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660431&req=5

f4: Tolerance of Ftu-UPT-LF to the real sample.Ftu-UPT-LF maintained sensitivity and specificity (*) and even improved thesensitivity by 10-fold (**) under the influence of powders and viscera ofgradient concentrations.
Mentions: The “white” powders in terrorist attacks (includingflour, fruit juice, gourmet powder, milk powder, putty powder, and sucrose),environmental material (soil), and viscera present in the surveillance center(including fresh and decomposed states) were prepared into variousconcentrations of solutions or homogenates to evaluate the tolerance ofFtu-UPT-LF. As Fig. 4 shows, Ftu-UPT-LF could tolerate 50,100, and200 mg · mL−1flour, fruit juice, gourmet powder, putty powder, and sucrose. With increasingconcentration, milk powder decreased the T/C ratios and destroyed thesensitivity of Ftu-UPT-LF at100 mg · mL−1.By contrast, soil increased the T/C ratios, resulting in a 10-fold improvementin sensitivity at50 mg · mL−1,and the specificity was destroyed at200 mg · mL−1.Ftu-UPT-LF showed excellent tolerance to all viscera with concentrations rangingfrom100 mg · mL−1to400 mg · mL−1.

Bottom Line: Except Shigella dysenteriae, UPT-LF showed excellent specificity to four strains that are also potential biowarfare/bioterrorism agents and 13 food-borne pathogenic strains.For practical utilization, the tolerance limits for seven powders and eight viscera were determined, and operation errors of liquid measurement demonstrated a minor influence on the strip.Ftu-UPT-LF is a candidate POCT method because of its excellent sensitivity, specificity, and stability in complex samples, as well as low operation error.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P.R. China.

ABSTRACT
Francisella tularensis is a potential biowarfare/bioterrorism agent and zoonotic pathogen that causes tularemia; thus, surveillance of F. tularensis and first-level emergency response using point-of-care testing (POCT) are essential. The UPT-LF POCT assay was established to quantitatively detect F. tularensis within 15 min, and the sensitivity of the assay was 10(4) CFU · mL(-1) (100 CFU/test). The linear quantitative range covered five orders of magnitude, and the coefficients of variation were less than 10%. Except Shigella dysenteriae, UPT-LF showed excellent specificity to four strains that are also potential biowarfare/bioterrorism agents and 13 food-borne pathogenic strains. Samples with pH 2-13, high ion strengths (≥ 2 mol · L(-1) solution of KCl and NaCl), high viscosities (≤ 50 mg · mL(-1) PEG20000 or ≥ 20% glycerol), and high concentrations of biomacromolecules (≥ 400 mg · mL(-1) bovine serum albumin or ≥ 80 mg · mL(-1) casein) showed little influence on the assay. For practical utilization, the tolerance limits for seven powders and eight viscera were determined, and operation errors of liquid measurement demonstrated a minor influence on the strip. Ftu-UPT-LF is a candidate POCT method because of its excellent sensitivity, specificity, and stability in complex samples, as well as low operation error.

No MeSH data available.


Related in: MedlinePlus