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Surveying the endomicrobiome and ectomicrobiome of bark beetles: The case of Dendroctonus simplex.

Durand AA, Bergeron A, Constant P, Buffet JP, Déziel E, Guertin C - Sci Rep (2015)

Bottom Line: The aim of this study was to characterize the bacterial microbiome associated with the eastern larch beetle, Dendroctonus simplex, using bacterial 16S rRNA gene pyrosequencing.The index of dissimilarity indicates that the bacterial microbiome associated with each environment constitutes exclusive groups.This study reports for the first time the identification of the eastern larch beetle microbiome.

View Article: PubMed Central - PubMed

Affiliation: INRS-Institut Armand-Frappier, Laval, QC, H7V1B7, Canada.

ABSTRACT
Many bark beetles belonging to the Dendroctonus genus carry bacterial and fungal microbiota, forming a symbiotic complex that helps the insect to colonize the subcortical environment of the host tree. However, the biodiversity of those bacteria at the surface of the cuticle or inside the body parts of bark beetles is not well established. The aim of this study was to characterize the bacterial microbiome associated with the eastern larch beetle, Dendroctonus simplex, using bacterial 16S rRNA gene pyrosequencing. The ecto- and endomicrobiome and the subcortical galleries were investigated. Several bacterial genera were identified, among which Pseudomonas, Serratia and Yersinia are associated with the surface of the beetle cuticle, and genera belonging to Enterobacteriaceae and Gammaproteobacteria with the interior of the insect body. The index of dissimilarity indicates that the bacterial microbiome associated with each environment constitutes exclusive groups. These results suggest the presence of distinct bacterial microbiota on the surface of the cuticle and the interior of D. simplex body. Additionally, the bacterial diversity identified in the galleries is substantially different from the ectomicrobiome, which could indicate a selection by the insect. This study reports for the first time the identification of the eastern larch beetle microbiome.

No MeSH data available.


Related in: MedlinePlus

Bacterial community composition of the eastern larch beetle and thegalleries.Only high quality filtered sequences were used to generate the heat map.Abundant OTUs (≥1% of the total abundance) are represented inthe chart. The non-abundant OTUs (<1%) are grouped in the categorynamed “others”. The heat map represents thelog-transformed relative abundance and grey representing the absence of theOTU. Number in parenthesis represents the number of OTUs associated witheach genus. Cluster of similarities regrouping the different samples isrepresented above the heat map, with each node supported by a Jackknifeanalysis with greater than 99.9% accuracy. “Ecto”,ectomicrobiome; “Gal”, microbiome of the galleries;“Endo”, endomicrobiome.
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f2: Bacterial community composition of the eastern larch beetle and thegalleries.Only high quality filtered sequences were used to generate the heat map.Abundant OTUs (≥1% of the total abundance) are represented inthe chart. The non-abundant OTUs (<1%) are grouped in the categorynamed “others”. The heat map represents thelog-transformed relative abundance and grey representing the absence of theOTU. Number in parenthesis represents the number of OTUs associated witheach genus. Cluster of similarities regrouping the different samples isrepresented above the heat map, with each node supported by a Jackknifeanalysis with greater than 99.9% accuracy. “Ecto”,ectomicrobiome; “Gal”, microbiome of the galleries;“Endo”, endomicrobiome.

Mentions: Each of the 278 OTUs was taxonomically identified using the RDP classifier. Mostof the identified OTUs belong to the phyla Acidobacteria, Bacteroidetes,Firmicutes and Proteobacteria (Fig. 2). Within theProteobacteria, Gammaproteobacteria were largely predominant, although a fewAlphaproteobacteria OTUs were also identified. Only one predominant bacterialgenus, Pseudomonas, was associated with the surface of the cuticle with95% of the sequences total abundance. Two other genera, Serratia andYersinia, were also considered abundant, representing respectively 2%and 1% of the sequences. Unclassified bacteria belonging to theEnterobacteriaceae family were also associated with the insect cuticle withabout 2% of the total sequences found.


Surveying the endomicrobiome and ectomicrobiome of bark beetles: The case of Dendroctonus simplex.

Durand AA, Bergeron A, Constant P, Buffet JP, Déziel E, Guertin C - Sci Rep (2015)

Bacterial community composition of the eastern larch beetle and thegalleries.Only high quality filtered sequences were used to generate the heat map.Abundant OTUs (≥1% of the total abundance) are represented inthe chart. The non-abundant OTUs (<1%) are grouped in the categorynamed “others”. The heat map represents thelog-transformed relative abundance and grey representing the absence of theOTU. Number in parenthesis represents the number of OTUs associated witheach genus. Cluster of similarities regrouping the different samples isrepresented above the heat map, with each node supported by a Jackknifeanalysis with greater than 99.9% accuracy. “Ecto”,ectomicrobiome; “Gal”, microbiome of the galleries;“Endo”, endomicrobiome.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660424&req=5

f2: Bacterial community composition of the eastern larch beetle and thegalleries.Only high quality filtered sequences were used to generate the heat map.Abundant OTUs (≥1% of the total abundance) are represented inthe chart. The non-abundant OTUs (<1%) are grouped in the categorynamed “others”. The heat map represents thelog-transformed relative abundance and grey representing the absence of theOTU. Number in parenthesis represents the number of OTUs associated witheach genus. Cluster of similarities regrouping the different samples isrepresented above the heat map, with each node supported by a Jackknifeanalysis with greater than 99.9% accuracy. “Ecto”,ectomicrobiome; “Gal”, microbiome of the galleries;“Endo”, endomicrobiome.
Mentions: Each of the 278 OTUs was taxonomically identified using the RDP classifier. Mostof the identified OTUs belong to the phyla Acidobacteria, Bacteroidetes,Firmicutes and Proteobacteria (Fig. 2). Within theProteobacteria, Gammaproteobacteria were largely predominant, although a fewAlphaproteobacteria OTUs were also identified. Only one predominant bacterialgenus, Pseudomonas, was associated with the surface of the cuticle with95% of the sequences total abundance. Two other genera, Serratia andYersinia, were also considered abundant, representing respectively 2%and 1% of the sequences. Unclassified bacteria belonging to theEnterobacteriaceae family were also associated with the insect cuticle withabout 2% of the total sequences found.

Bottom Line: The aim of this study was to characterize the bacterial microbiome associated with the eastern larch beetle, Dendroctonus simplex, using bacterial 16S rRNA gene pyrosequencing.The index of dissimilarity indicates that the bacterial microbiome associated with each environment constitutes exclusive groups.This study reports for the first time the identification of the eastern larch beetle microbiome.

View Article: PubMed Central - PubMed

Affiliation: INRS-Institut Armand-Frappier, Laval, QC, H7V1B7, Canada.

ABSTRACT
Many bark beetles belonging to the Dendroctonus genus carry bacterial and fungal microbiota, forming a symbiotic complex that helps the insect to colonize the subcortical environment of the host tree. However, the biodiversity of those bacteria at the surface of the cuticle or inside the body parts of bark beetles is not well established. The aim of this study was to characterize the bacterial microbiome associated with the eastern larch beetle, Dendroctonus simplex, using bacterial 16S rRNA gene pyrosequencing. The ecto- and endomicrobiome and the subcortical galleries were investigated. Several bacterial genera were identified, among which Pseudomonas, Serratia and Yersinia are associated with the surface of the beetle cuticle, and genera belonging to Enterobacteriaceae and Gammaproteobacteria with the interior of the insect body. The index of dissimilarity indicates that the bacterial microbiome associated with each environment constitutes exclusive groups. These results suggest the presence of distinct bacterial microbiota on the surface of the cuticle and the interior of D. simplex body. Additionally, the bacterial diversity identified in the galleries is substantially different from the ectomicrobiome, which could indicate a selection by the insect. This study reports for the first time the identification of the eastern larch beetle microbiome.

No MeSH data available.


Related in: MedlinePlus