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Direct visualization of dispersed lipid bicontinuous cubic phases by cryo-electron tomography.

Demurtas D, Guichard P, Martiel I, Mezzenga R, Hébert C, Sagalowicz L - Nat Commun (2015)

Bottom Line: Moreover, their biocompatibility is attractive for nutrient or drug delivery system applications.Here the three-dimensional organization of dispersed cubic lipid self-assembled phases is fully revealed by cryo-electron tomography and compared with simulated structures.Therefore, compositional gradients within cubosomes are inferred, with a lipid bilayer separating at least one water channel set from the external aqueous phase.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Centre for Electron Microscopy, Swiss Federal Institute of Technology (EPFL), Lausanne 1015, Switzerland.

ABSTRACT
Bulk and dispersed cubic liquid crystalline phases (cubosomes), present in the body and in living cell membranes, are believed to play an essential role in biological phenomena. Moreover, their biocompatibility is attractive for nutrient or drug delivery system applications. Here the three-dimensional organization of dispersed cubic lipid self-assembled phases is fully revealed by cryo-electron tomography and compared with simulated structures. It is demonstrated that the interior is constituted of a perfect bicontinuous cubic phase, while the outside shows interlamellar attachments, which represent a transition state between the liquid crystalline interior phase and the outside vesicular structure. Therefore, compositional gradients within cubosomes are inferred, with a lipid bilayer separating at least one water channel set from the external aqueous phase. This is crucial to understand and enhance controlled release of target molecules and calls for a revision of postulated transport mechanisms from cubosomes to the aqueous phase.

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Comparison between mathematical model and cryo-ET 3D reconstruction.(a) Minimal surface type of cubic structure. (b) Comparison between the decomposition series of the model and the stack series obtained by CET of the liquid crystal region. (c) Mathematical model of the unit cell and their 3D reconstruction projections obtained by CET. (d) 3D reconstruction by CET.
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f3: Comparison between mathematical model and cryo-ET 3D reconstruction.(a) Minimal surface type of cubic structure. (b) Comparison between the decomposition series of the model and the stack series obtained by CET of the liquid crystal region. (c) Mathematical model of the unit cell and their 3D reconstruction projections obtained by CET. (d) 3D reconstruction by CET.

Mentions: Figure 3a gives the 3D image of the P surface obtained using equation (1).


Direct visualization of dispersed lipid bicontinuous cubic phases by cryo-electron tomography.

Demurtas D, Guichard P, Martiel I, Mezzenga R, Hébert C, Sagalowicz L - Nat Commun (2015)

Comparison between mathematical model and cryo-ET 3D reconstruction.(a) Minimal surface type of cubic structure. (b) Comparison between the decomposition series of the model and the stack series obtained by CET of the liquid crystal region. (c) Mathematical model of the unit cell and their 3D reconstruction projections obtained by CET. (d) 3D reconstruction by CET.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660369&req=5

f3: Comparison between mathematical model and cryo-ET 3D reconstruction.(a) Minimal surface type of cubic structure. (b) Comparison between the decomposition series of the model and the stack series obtained by CET of the liquid crystal region. (c) Mathematical model of the unit cell and their 3D reconstruction projections obtained by CET. (d) 3D reconstruction by CET.
Mentions: Figure 3a gives the 3D image of the P surface obtained using equation (1).

Bottom Line: Moreover, their biocompatibility is attractive for nutrient or drug delivery system applications.Here the three-dimensional organization of dispersed cubic lipid self-assembled phases is fully revealed by cryo-electron tomography and compared with simulated structures.Therefore, compositional gradients within cubosomes are inferred, with a lipid bilayer separating at least one water channel set from the external aqueous phase.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Centre for Electron Microscopy, Swiss Federal Institute of Technology (EPFL), Lausanne 1015, Switzerland.

ABSTRACT
Bulk and dispersed cubic liquid crystalline phases (cubosomes), present in the body and in living cell membranes, are believed to play an essential role in biological phenomena. Moreover, their biocompatibility is attractive for nutrient or drug delivery system applications. Here the three-dimensional organization of dispersed cubic lipid self-assembled phases is fully revealed by cryo-electron tomography and compared with simulated structures. It is demonstrated that the interior is constituted of a perfect bicontinuous cubic phase, while the outside shows interlamellar attachments, which represent a transition state between the liquid crystalline interior phase and the outside vesicular structure. Therefore, compositional gradients within cubosomes are inferred, with a lipid bilayer separating at least one water channel set from the external aqueous phase. This is crucial to understand and enhance controlled release of target molecules and calls for a revision of postulated transport mechanisms from cubosomes to the aqueous phase.

Show MeSH
Related in: MedlinePlus