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Impact of a wastewater treatment plant on microbial community composition and function in a hyporheic zone of a eutrophic river.

Atashgahi S, Aydin R, Dimitrov MR, Sipkema D, Hamonts K, Lahti L, Maphosa F, Kruse T, Saccenti E, Springael D, Dejonghe W, Smidt H - Sci Rep (2015)

Bottom Line: Pyrosequencing of bacterial 16S rRNA gene fragments in sediment cores showed reduced relative abundance of heterotrophs and fermenters such as Chloroflexi and Firmicutes in more oxic and nutrient poor post-WWTP sediments.This transition in the trophic state of the hyporheic sediments reduced but did not abolish the VC respiration potential in the post-WWTP sediments as an important hyporheic sediment function.Our results highlight effective nutrient load reduction and parallel microbial ecological state restoration of a human-stressed urban river as a result of installation of a WWTP.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.

ABSTRACT
The impact of the installation of a technologically advanced wastewater treatment plant (WWTP) on the benthic microbial community of a vinyl chloride (VC) impacted eutrophic river was examined two years before, and three and four years after installation of the WWTP. Reduced dissolved organic carbon and increased dissolved oxygen concentrations in surface water and reduced total organic carbon and total nitrogen content in the sediment were recorded in the post-WWTP samples. Pyrosequencing of bacterial 16S rRNA gene fragments in sediment cores showed reduced relative abundance of heterotrophs and fermenters such as Chloroflexi and Firmicutes in more oxic and nutrient poor post-WWTP sediments. Similarly, quantitative PCR analysis showed 1-3 orders of magnitude reduction in phylogenetic and functional genes of sulphate reducers, denitrifiers, ammonium oxidizers, methanogens and VC-respiring Dehalococcoides mccartyi. In contrast, members of Proteobacteria adapted to nutrient-poor conditions were enriched in post-WWTP samples. This transition in the trophic state of the hyporheic sediments reduced but did not abolish the VC respiration potential in the post-WWTP sediments as an important hyporheic sediment function. Our results highlight effective nutrient load reduction and parallel microbial ecological state restoration of a human-stressed urban river as a result of installation of a WWTP.

No MeSH data available.


Related in: MedlinePlus

Diversity indices.Alpha diversity (Shannon) and richness (Chao1 and OTU count) indices at different depths in the sediment core samples.
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f4: Diversity indices.Alpha diversity (Shannon) and richness (Chao1 and OTU count) indices at different depths in the sediment core samples.

Mentions: In the sediment cores sampled in 2005, the alpha diversity (Shannon) and richness (Chao1 and OTU count) indices did not show significant difference between two cores (Fig. 4, Supplementary Table S11). Similarly, the alpha diversity and richness of the post-WWTP samples of core-B of 2010 and core-B of 2011 did not show significant differences with the pre-WWTP samples. However, the alpha diversity and richness indices were the highest and lowest in the core-A of 2010 and core-A of 2011, respectively (Fig. 4, Supplementary Table S11).


Impact of a wastewater treatment plant on microbial community composition and function in a hyporheic zone of a eutrophic river.

Atashgahi S, Aydin R, Dimitrov MR, Sipkema D, Hamonts K, Lahti L, Maphosa F, Kruse T, Saccenti E, Springael D, Dejonghe W, Smidt H - Sci Rep (2015)

Diversity indices.Alpha diversity (Shannon) and richness (Chao1 and OTU count) indices at different depths in the sediment core samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660315&req=5

f4: Diversity indices.Alpha diversity (Shannon) and richness (Chao1 and OTU count) indices at different depths in the sediment core samples.
Mentions: In the sediment cores sampled in 2005, the alpha diversity (Shannon) and richness (Chao1 and OTU count) indices did not show significant difference between two cores (Fig. 4, Supplementary Table S11). Similarly, the alpha diversity and richness of the post-WWTP samples of core-B of 2010 and core-B of 2011 did not show significant differences with the pre-WWTP samples. However, the alpha diversity and richness indices were the highest and lowest in the core-A of 2010 and core-A of 2011, respectively (Fig. 4, Supplementary Table S11).

Bottom Line: Pyrosequencing of bacterial 16S rRNA gene fragments in sediment cores showed reduced relative abundance of heterotrophs and fermenters such as Chloroflexi and Firmicutes in more oxic and nutrient poor post-WWTP sediments.This transition in the trophic state of the hyporheic sediments reduced but did not abolish the VC respiration potential in the post-WWTP sediments as an important hyporheic sediment function.Our results highlight effective nutrient load reduction and parallel microbial ecological state restoration of a human-stressed urban river as a result of installation of a WWTP.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.

ABSTRACT
The impact of the installation of a technologically advanced wastewater treatment plant (WWTP) on the benthic microbial community of a vinyl chloride (VC) impacted eutrophic river was examined two years before, and three and four years after installation of the WWTP. Reduced dissolved organic carbon and increased dissolved oxygen concentrations in surface water and reduced total organic carbon and total nitrogen content in the sediment were recorded in the post-WWTP samples. Pyrosequencing of bacterial 16S rRNA gene fragments in sediment cores showed reduced relative abundance of heterotrophs and fermenters such as Chloroflexi and Firmicutes in more oxic and nutrient poor post-WWTP sediments. Similarly, quantitative PCR analysis showed 1-3 orders of magnitude reduction in phylogenetic and functional genes of sulphate reducers, denitrifiers, ammonium oxidizers, methanogens and VC-respiring Dehalococcoides mccartyi. In contrast, members of Proteobacteria adapted to nutrient-poor conditions were enriched in post-WWTP samples. This transition in the trophic state of the hyporheic sediments reduced but did not abolish the VC respiration potential in the post-WWTP sediments as an important hyporheic sediment function. Our results highlight effective nutrient load reduction and parallel microbial ecological state restoration of a human-stressed urban river as a result of installation of a WWTP.

No MeSH data available.


Related in: MedlinePlus