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The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China.

Li H, Yang Q, Li J, Gao H, Li P, Zhou H - Sci Rep (2015)

Bottom Line: We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C).Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected.AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.

ABSTRACT
Using a culture-independent method that combines CARD-FISH, qPCR and 16S rDNA, we investigated the abundance, community structure and diversity of microbes along a steep thermal gradient (50-90 °C) in the Tengchong Geothermal Field. We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C). Under low-temperature conditions (52.3-74.6 °C), the microbial communities were dominated by Bacteria, which accounted for 60-80% of the total number of cells. At 74.6 °C, Archaea were dominant, and at 90.8 °C, they accounted for more than 90% of the total number of cells. Additionally, the microbial communities at high temperatures (74.6-90.8 °C) were substantially simpler than those at the low-temperature sites. Only a few genera (e.g., bacterial Caldisericum, Thermotoga and Thermoanaerobacter, archaeal Vulcanisaeta and Hyperthermus) often dominated in high-temperature environments. Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected. AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

No MeSH data available.


Related in: MedlinePlus

Temperature versus Shannon-Weaver index for each site in the current study.For this hot spring, an inverse linear trend (dotted line) between T and the Shannon–Weaver index is evident.
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f3: Temperature versus Shannon-Weaver index for each site in the current study.For this hot spring, an inverse linear trend (dotted line) between T and the Shannon–Weaver index is evident.

Mentions: A very obvious change in soil community diversity was apparent across the thermal gradient (Fig. 3). The microorganismal community profiles at the hottest soil layer were substantially simpler than those of the lower temperature sites. Peak diversity for all samples was observed at 52.3 °C (Shannon–Weaver index: Bacteria, 2.89; Archaea, 1.87), with lower diversity under hotter conditions (Fig. 3). The bacterial diversity analysis revealed that the community in this thermal region was dominated by five phyla: Proteobacteria, Firmicutes, Nitrospirae, Thermotogae and Cyanobacteria. The greatest diversity was observed in the phylum Proteobacteria, with eleven genera present belonging to the classes Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria (Table S1).


The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China.

Li H, Yang Q, Li J, Gao H, Li P, Zhou H - Sci Rep (2015)

Temperature versus Shannon-Weaver index for each site in the current study.For this hot spring, an inverse linear trend (dotted line) between T and the Shannon–Weaver index is evident.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660298&req=5

f3: Temperature versus Shannon-Weaver index for each site in the current study.For this hot spring, an inverse linear trend (dotted line) between T and the Shannon–Weaver index is evident.
Mentions: A very obvious change in soil community diversity was apparent across the thermal gradient (Fig. 3). The microorganismal community profiles at the hottest soil layer were substantially simpler than those of the lower temperature sites. Peak diversity for all samples was observed at 52.3 °C (Shannon–Weaver index: Bacteria, 2.89; Archaea, 1.87), with lower diversity under hotter conditions (Fig. 3). The bacterial diversity analysis revealed that the community in this thermal region was dominated by five phyla: Proteobacteria, Firmicutes, Nitrospirae, Thermotogae and Cyanobacteria. The greatest diversity was observed in the phylum Proteobacteria, with eleven genera present belonging to the classes Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria (Table S1).

Bottom Line: We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C).Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected.AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.

ABSTRACT
Using a culture-independent method that combines CARD-FISH, qPCR and 16S rDNA, we investigated the abundance, community structure and diversity of microbes along a steep thermal gradient (50-90 °C) in the Tengchong Geothermal Field. We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C). Under low-temperature conditions (52.3-74.6 °C), the microbial communities were dominated by Bacteria, which accounted for 60-80% of the total number of cells. At 74.6 °C, Archaea were dominant, and at 90.8 °C, they accounted for more than 90% of the total number of cells. Additionally, the microbial communities at high temperatures (74.6-90.8 °C) were substantially simpler than those at the low-temperature sites. Only a few genera (e.g., bacterial Caldisericum, Thermotoga and Thermoanaerobacter, archaeal Vulcanisaeta and Hyperthermus) often dominated in high-temperature environments. Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected. AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

No MeSH data available.


Related in: MedlinePlus