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The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China.

Li H, Yang Q, Li J, Gao H, Li P, Zhou H - Sci Rep (2015)

Bottom Line: We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C).Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected.AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.

ABSTRACT
Using a culture-independent method that combines CARD-FISH, qPCR and 16S rDNA, we investigated the abundance, community structure and diversity of microbes along a steep thermal gradient (50-90 °C) in the Tengchong Geothermal Field. We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C). Under low-temperature conditions (52.3-74.6 °C), the microbial communities were dominated by Bacteria, which accounted for 60-80% of the total number of cells. At 74.6 °C, Archaea were dominant, and at 90.8 °C, they accounted for more than 90% of the total number of cells. Additionally, the microbial communities at high temperatures (74.6-90.8 °C) were substantially simpler than those at the low-temperature sites. Only a few genera (e.g., bacterial Caldisericum, Thermotoga and Thermoanaerobacter, archaeal Vulcanisaeta and Hyperthermus) often dominated in high-temperature environments. Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected. AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of CARD-FISH stained samples.(A) DAPI stained. (B) Probe EUB338 targeted most of Bacteria. (C) Probe Arch958 targeted most of Archaea. (D) Probe Cren512 targeted most of AOA. Magnification = 200×, scale bar = 100 μm.
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f1: Photomicrographs of CARD-FISH stained samples.(A) DAPI stained. (B) Probe EUB338 targeted most of Bacteria. (C) Probe Arch958 targeted most of Archaea. (D) Probe Cren512 targeted most of AOA. Magnification = 200×, scale bar = 100 μm.

Mentions: In this study, we used CARD-FISH to investigate the microbial distribution of samples of different temperatures. At 2 to 5 ng/μL, the probe EUB338 (Table 1, Fig. 1B, Fig. S1) mainly targeted Bacteria, whereas the probe Arch 915 (Fig. 1C) primarily targeted Archaea, and the probe Cren512 (Fig. 1D) mostly targeted AOA21. The average sum of Archaea plus Bacteria cell numbers detected by 16S rDNA fluorescent probes accounted for 60–95% of the total DAPI-stained cell numbers.


The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China.

Li H, Yang Q, Li J, Gao H, Li P, Zhou H - Sci Rep (2015)

Photomicrographs of CARD-FISH stained samples.(A) DAPI stained. (B) Probe EUB338 targeted most of Bacteria. (C) Probe Arch958 targeted most of Archaea. (D) Probe Cren512 targeted most of AOA. Magnification = 200×, scale bar = 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660298&req=5

f1: Photomicrographs of CARD-FISH stained samples.(A) DAPI stained. (B) Probe EUB338 targeted most of Bacteria. (C) Probe Arch958 targeted most of Archaea. (D) Probe Cren512 targeted most of AOA. Magnification = 200×, scale bar = 100 μm.
Mentions: In this study, we used CARD-FISH to investigate the microbial distribution of samples of different temperatures. At 2 to 5 ng/μL, the probe EUB338 (Table 1, Fig. 1B, Fig. S1) mainly targeted Bacteria, whereas the probe Arch 915 (Fig. 1C) primarily targeted Archaea, and the probe Cren512 (Fig. 1D) mostly targeted AOA21. The average sum of Archaea plus Bacteria cell numbers detected by 16S rDNA fluorescent probes accounted for 60–95% of the total DAPI-stained cell numbers.

Bottom Line: We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C).Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected.AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.

ABSTRACT
Using a culture-independent method that combines CARD-FISH, qPCR and 16S rDNA, we investigated the abundance, community structure and diversity of microbes along a steep thermal gradient (50-90 °C) in the Tengchong Geothermal Field. We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C). Under low-temperature conditions (52.3-74.6 °C), the microbial communities were dominated by Bacteria, which accounted for 60-80% of the total number of cells. At 74.6 °C, Archaea were dominant, and at 90.8 °C, they accounted for more than 90% of the total number of cells. Additionally, the microbial communities at high temperatures (74.6-90.8 °C) were substantially simpler than those at the low-temperature sites. Only a few genera (e.g., bacterial Caldisericum, Thermotoga and Thermoanaerobacter, archaeal Vulcanisaeta and Hyperthermus) often dominated in high-temperature environments. Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected. AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

No MeSH data available.


Related in: MedlinePlus