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Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction.

Mendoza O, Elezgaray J, Mergny JL - Data Brief (2015)

Bottom Line: An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction.We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case.In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA).

View Article: PubMed Central - PubMed

Affiliation: University of Bordeaux, 33600 Bordeaux, France ; INSERM, ARNA Laboratory, U869, IECB, F-33600 Pessac, France.

ABSTRACT
This article describes additional data related to a research article entitled "Kinetics of Quadruplex to Duplex Conversion" (Mendoza et al. 2015 [1]). We followed the opening reaction of a series of intramolecular G-quadruplex structures by the addition of their corresponding complementary strand. Fluorolabeled complementary strands allowed to monitor the reaction in real-time. An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction. We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case. In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA).

No MeSH data available.


CD spectra in 10 mM cacodylate buffer (pH 7.2) cmyc1 and cmyc2 containing 100 mM LiCl and no KCl.
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f0030: CD spectra in 10 mM cacodylate buffer (pH 7.2) cmyc1 and cmyc2 containing 100 mM LiCl and no KCl.

Mentions: Circular dichroism was used to confirm the presence of a G4 motif in TBA2, cmyc1, cmyc2, htelo1 and htelo2 at the lowest KCl concentration considered in this study (thus 20 mM KCl, 1 mM KCl, 0.5 mM KCl, 100 mM KCl and 100 mM KCl respectively). Fig. 5, Fig. 6 show that in all the cases, a typical signature of a quadruplex motif was found. Finally Table 1 shows the calculated kinetic constants for the opening reaction of the quadruplex motif htelo1.


Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction.

Mendoza O, Elezgaray J, Mergny JL - Data Brief (2015)

CD spectra in 10 mM cacodylate buffer (pH 7.2) cmyc1 and cmyc2 containing 100 mM LiCl and no KCl.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660241&req=5

f0030: CD spectra in 10 mM cacodylate buffer (pH 7.2) cmyc1 and cmyc2 containing 100 mM LiCl and no KCl.
Mentions: Circular dichroism was used to confirm the presence of a G4 motif in TBA2, cmyc1, cmyc2, htelo1 and htelo2 at the lowest KCl concentration considered in this study (thus 20 mM KCl, 1 mM KCl, 0.5 mM KCl, 100 mM KCl and 100 mM KCl respectively). Fig. 5, Fig. 6 show that in all the cases, a typical signature of a quadruplex motif was found. Finally Table 1 shows the calculated kinetic constants for the opening reaction of the quadruplex motif htelo1.

Bottom Line: An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction.We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case.In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA).

View Article: PubMed Central - PubMed

Affiliation: University of Bordeaux, 33600 Bordeaux, France ; INSERM, ARNA Laboratory, U869, IECB, F-33600 Pessac, France.

ABSTRACT
This article describes additional data related to a research article entitled "Kinetics of Quadruplex to Duplex Conversion" (Mendoza et al. 2015 [1]). We followed the opening reaction of a series of intramolecular G-quadruplex structures by the addition of their corresponding complementary strand. Fluorolabeled complementary strands allowed to monitor the reaction in real-time. An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction. We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case. In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA).

No MeSH data available.