Limits...
Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction.

Mendoza O, Elezgaray J, Mergny JL - Data Brief (2015)

Bottom Line: An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction.We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case.In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA).

View Article: PubMed Central - PubMed

Affiliation: University of Bordeaux, 33600 Bordeaux, France ; INSERM, ARNA Laboratory, U869, IECB, F-33600 Pessac, France.

ABSTRACT
This article describes additional data related to a research article entitled "Kinetics of Quadruplex to Duplex Conversion" (Mendoza et al. 2015 [1]). We followed the opening reaction of a series of intramolecular G-quadruplex structures by the addition of their corresponding complementary strand. Fluorolabeled complementary strands allowed to monitor the reaction in real-time. An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction. We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case. In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA).

No MeSH data available.


Melting profiles (normalized absorbance at 260 nm) obtained for 4 μM DNA duplex: dx_TBA2, dx_TBA2, dx_cmyc1, dx_cmyc2, dx_htelo1 and dx_htelo2.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4660241&req=5

f0020: Melting profiles (normalized absorbance at 260 nm) obtained for 4 μM DNA duplex: dx_TBA2, dx_TBA2, dx_cmyc1, dx_cmyc2, dx_htelo1 and dx_htelo2.

Mentions: The thermal stability of all DNA structures considered in this research is also provided. The Tm of the G-quadruplex motifs TBA2, cmyc1, cmyc2, htelo1 and htelo2 and the duplex structures dx_TBA2, dx_TBA2, dx_cmyc1, dx_cmyc2, dx_htelo1 and dx_htelo2 was calculated. Fig. 3, Fig. 4 show the thermal melting profiles for the G-quadruplex and duplex structures respectively.


Spectroscopic data for the G-quadruplex DNA to duplex DNA reaction.

Mendoza O, Elezgaray J, Mergny JL - Data Brief (2015)

Melting profiles (normalized absorbance at 260 nm) obtained for 4 μM DNA duplex: dx_TBA2, dx_TBA2, dx_cmyc1, dx_cmyc2, dx_htelo1 and dx_htelo2.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660241&req=5

f0020: Melting profiles (normalized absorbance at 260 nm) obtained for 4 μM DNA duplex: dx_TBA2, dx_TBA2, dx_cmyc1, dx_cmyc2, dx_htelo1 and dx_htelo2.
Mentions: The thermal stability of all DNA structures considered in this research is also provided. The Tm of the G-quadruplex motifs TBA2, cmyc1, cmyc2, htelo1 and htelo2 and the duplex structures dx_TBA2, dx_TBA2, dx_cmyc1, dx_cmyc2, dx_htelo1 and dx_htelo2 was calculated. Fig. 3, Fig. 4 show the thermal melting profiles for the G-quadruplex and duplex structures respectively.

Bottom Line: An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction.We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case.In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA).

View Article: PubMed Central - PubMed

Affiliation: University of Bordeaux, 33600 Bordeaux, France ; INSERM, ARNA Laboratory, U869, IECB, F-33600 Pessac, France.

ABSTRACT
This article describes additional data related to a research article entitled "Kinetics of Quadruplex to Duplex Conversion" (Mendoza et al. 2015 [1]). We followed the opening reaction of a series of intramolecular G-quadruplex structures by the addition of their corresponding complementary strand. Fluorolabeled complementary strands allowed to monitor the reaction in real-time. An adapted kinetic model was then applied in order to obtain the kinetic parameters of this reaction. We present a series of kinetic traces providing raw data of the G4 opening reaction and the fitting model applied in every case. In addition CD spectra and UV melting data is also provided to confirm the stability of all the DNA structures considered (G-quadruplex and duplex DNA).

No MeSH data available.