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Expression and regulation of redoxins at nociceptive signaling sites after sciatic nerve injury in mice.

Valek L, Kanngießer M, Tegeder I - Data Brief (2015)

Bottom Line: The redoxin immunoreactivity was quantified with ImageJ.For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity transformed to rainbow pseudocolors.In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord and some redoxin examples of the thalamus are presented.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Pharmacology/ZAFES, Goethe-University Hospital, Frankfurt, Germany.

ABSTRACT
Injury of the sciatic nerve results in regulations of pro- and anti-oxidative enzymes at sites of nociceptive signaling including the injured nerve, dorsal root ganglia (DRGs), dorsal horn of the spinal cord, thalamus and somatosensory cortex (Valek et al., 2015) [1]. The present DiB paper shows immunohistochemistry of redoxins including peroxiredoxins (Prdx1-6), glutaredoxins (Glrx1, 2, 3, 5), thioredoxins (Txn1, 2) and thioredoxin reductases (Txnrd1, 2) in the DRGs, spinal cord and sciatic nerve and thalamus in naïve mice and 7 days after Spared sciatic Nerve Injury (SNI) in control mice (Hif1α-flfl) and in mice with a specific deletion of hypoxia inducible factor 1 alpha (SNS-HIF1α(-/-)) in DRG neurons. The sciatic nerves were immunostained for the respective redoxins and counterstained with hematoxylin. The redoxin immunoreactivity was quantified with ImageJ. For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity transformed to rainbow pseudocolors. In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord and some redoxin examples of the thalamus are presented.

No MeSH data available.


Related in: MedlinePlus

Exemplary immunohistology of redoxins (red, hematoxylin counterstain in blue) in the thalamus 7 days after Spared Nerve Injury (SNI) of the sciatic nerve in SNS-HIF1α−/− and HIF1α-flfl mice. SNS-HIF1α−/− have a cre/loxP mediated deletion of hypoxia inducible factor 1 alpha specifically in sensory neurons of the dorsal root ganglia (DRGs). The right panel shows a higher magnification. Slides were developed with the Streptavidin–HRP system using red AEC as substrate. Scale bars 50 and 20 µm.
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f0040: Exemplary immunohistology of redoxins (red, hematoxylin counterstain in blue) in the thalamus 7 days after Spared Nerve Injury (SNI) of the sciatic nerve in SNS-HIF1α−/− and HIF1α-flfl mice. SNS-HIF1α−/− have a cre/loxP mediated deletion of hypoxia inducible factor 1 alpha specifically in sensory neurons of the dorsal root ganglia (DRGs). The right panel shows a higher magnification. Slides were developed with the Streptavidin–HRP system using red AEC as substrate. Scale bars 50 and 20 µm.

Mentions: The present DiB paper shows immunohistochemistry of redoxins including peroxiredoxins (Prdx1–6), glutaredoxins (Glrx1, 2, 3, 5), thioredoxins (Txn1, 2) and thioredoxin reductases (Txnrd1, 2) in the DRGs Figs. 1 and 2), spinal cord (Fig. 1, Fig. 2, Fig. 7), sciatic nerve (Fig. 3, Fig. 4, Fig. 5, quantification Fig. 6) and thalamus (Fig. 8) in naïve mice and 7 days after Spared sciatic Nerve Injury (SNI) in control mice (Hif1α-flfl) and in mice with a specific deletion of hypoxia inducible factor 1 alpha (SNS-HIF1α−/−) in DRG neurons. The sciatic nerves were immunostained for the respective redoxins and counterstained with hematoxylin. The redoxin immunoreactivity was quantified with ImageJ. For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity [1] transformed to rainbow pseudocolors (Fig. 2). In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord (Fig. 7) and some redoxin examples of the thalamus (Fig. 8) are presented. Characteristics of the antibodies are listed in Table 1 along with some features of the redoxins.


Expression and regulation of redoxins at nociceptive signaling sites after sciatic nerve injury in mice.

Valek L, Kanngießer M, Tegeder I - Data Brief (2015)

Exemplary immunohistology of redoxins (red, hematoxylin counterstain in blue) in the thalamus 7 days after Spared Nerve Injury (SNI) of the sciatic nerve in SNS-HIF1α−/− and HIF1α-flfl mice. SNS-HIF1α−/− have a cre/loxP mediated deletion of hypoxia inducible factor 1 alpha specifically in sensory neurons of the dorsal root ganglia (DRGs). The right panel shows a higher magnification. Slides were developed with the Streptavidin–HRP system using red AEC as substrate. Scale bars 50 and 20 µm.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660239&req=5

f0040: Exemplary immunohistology of redoxins (red, hematoxylin counterstain in blue) in the thalamus 7 days after Spared Nerve Injury (SNI) of the sciatic nerve in SNS-HIF1α−/− and HIF1α-flfl mice. SNS-HIF1α−/− have a cre/loxP mediated deletion of hypoxia inducible factor 1 alpha specifically in sensory neurons of the dorsal root ganglia (DRGs). The right panel shows a higher magnification. Slides were developed with the Streptavidin–HRP system using red AEC as substrate. Scale bars 50 and 20 µm.
Mentions: The present DiB paper shows immunohistochemistry of redoxins including peroxiredoxins (Prdx1–6), glutaredoxins (Glrx1, 2, 3, 5), thioredoxins (Txn1, 2) and thioredoxin reductases (Txnrd1, 2) in the DRGs Figs. 1 and 2), spinal cord (Fig. 1, Fig. 2, Fig. 7), sciatic nerve (Fig. 3, Fig. 4, Fig. 5, quantification Fig. 6) and thalamus (Fig. 8) in naïve mice and 7 days after Spared sciatic Nerve Injury (SNI) in control mice (Hif1α-flfl) and in mice with a specific deletion of hypoxia inducible factor 1 alpha (SNS-HIF1α−/−) in DRG neurons. The sciatic nerves were immunostained for the respective redoxins and counterstained with hematoxylin. The redoxin immunoreactivity was quantified with ImageJ. For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity [1] transformed to rainbow pseudocolors (Fig. 2). In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord (Fig. 7) and some redoxin examples of the thalamus (Fig. 8) are presented. Characteristics of the antibodies are listed in Table 1 along with some features of the redoxins.

Bottom Line: The redoxin immunoreactivity was quantified with ImageJ.For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity transformed to rainbow pseudocolors.In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord and some redoxin examples of the thalamus are presented.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Pharmacology/ZAFES, Goethe-University Hospital, Frankfurt, Germany.

ABSTRACT
Injury of the sciatic nerve results in regulations of pro- and anti-oxidative enzymes at sites of nociceptive signaling including the injured nerve, dorsal root ganglia (DRGs), dorsal horn of the spinal cord, thalamus and somatosensory cortex (Valek et al., 2015) [1]. The present DiB paper shows immunohistochemistry of redoxins including peroxiredoxins (Prdx1-6), glutaredoxins (Glrx1, 2, 3, 5), thioredoxins (Txn1, 2) and thioredoxin reductases (Txnrd1, 2) in the DRGs, spinal cord and sciatic nerve and thalamus in naïve mice and 7 days after Spared sciatic Nerve Injury (SNI) in control mice (Hif1α-flfl) and in mice with a specific deletion of hypoxia inducible factor 1 alpha (SNS-HIF1α(-/-)) in DRG neurons. The sciatic nerves were immunostained for the respective redoxins and counterstained with hematoxylin. The redoxin immunoreactivity was quantified with ImageJ. For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity transformed to rainbow pseudocolors. In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord and some redoxin examples of the thalamus are presented.

No MeSH data available.


Related in: MedlinePlus