Limits...
Expression and regulation of redoxins at nociceptive signaling sites after sciatic nerve injury in mice.

Valek L, Kanngießer M, Tegeder I - Data Brief (2015)

Bottom Line: The redoxin immunoreactivity was quantified with ImageJ.For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity transformed to rainbow pseudocolors.In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord and some redoxin examples of the thalamus are presented.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Pharmacology/ZAFES, Goethe-University Hospital, Frankfurt, Germany.

ABSTRACT
Injury of the sciatic nerve results in regulations of pro- and anti-oxidative enzymes at sites of nociceptive signaling including the injured nerve, dorsal root ganglia (DRGs), dorsal horn of the spinal cord, thalamus and somatosensory cortex (Valek et al., 2015) [1]. The present DiB paper shows immunohistochemistry of redoxins including peroxiredoxins (Prdx1-6), glutaredoxins (Glrx1, 2, 3, 5), thioredoxins (Txn1, 2) and thioredoxin reductases (Txnrd1, 2) in the DRGs, spinal cord and sciatic nerve and thalamus in naïve mice and 7 days after Spared sciatic Nerve Injury (SNI) in control mice (Hif1α-flfl) and in mice with a specific deletion of hypoxia inducible factor 1 alpha (SNS-HIF1α(-/-)) in DRG neurons. The sciatic nerves were immunostained for the respective redoxins and counterstained with hematoxylin. The redoxin immunoreactivity was quantified with ImageJ. For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity transformed to rainbow pseudocolors. In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord and some redoxin examples of the thalamus are presented.

No MeSH data available.


Related in: MedlinePlus

Quantification of redoxin histology of the sciatic nerve with WCIF ImageJ. Mean pixel intensities were determined after substraction of background, RGB split into its channels and threshold settings based on negative control images using “auto” settings. The analysis did not differentiate between different types of cells and is a global readout for immunoreactivities in axonal fibers, Schwann cells and infiltrating immune cells. Pixel intensities were compared with one-way ANOVA for each redoxin separately. In case of significance, groups were mutually compared employing a Sidak correction of alpha, which was set at 0.05 for all comparisons. Asterisks indicate significant differences versus the respective naive animals. The analysis was based on results of 3 animals per group.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4660239&req=5

f0030: Quantification of redoxin histology of the sciatic nerve with WCIF ImageJ. Mean pixel intensities were determined after substraction of background, RGB split into its channels and threshold settings based on negative control images using “auto” settings. The analysis did not differentiate between different types of cells and is a global readout for immunoreactivities in axonal fibers, Schwann cells and infiltrating immune cells. Pixel intensities were compared with one-way ANOVA for each redoxin separately. In case of significance, groups were mutually compared employing a Sidak correction of alpha, which was set at 0.05 for all comparisons. Asterisks indicate significant differences versus the respective naive animals. The analysis was based on results of 3 animals per group.

Mentions: The present DiB paper shows immunohistochemistry of redoxins including peroxiredoxins (Prdx1–6), glutaredoxins (Glrx1, 2, 3, 5), thioredoxins (Txn1, 2) and thioredoxin reductases (Txnrd1, 2) in the DRGs Figs. 1 and 2), spinal cord (Fig. 1, Fig. 2, Fig. 7), sciatic nerve (Fig. 3, Fig. 4, Fig. 5, quantification Fig. 6) and thalamus (Fig. 8) in naïve mice and 7 days after Spared sciatic Nerve Injury (SNI) in control mice (Hif1α-flfl) and in mice with a specific deletion of hypoxia inducible factor 1 alpha (SNS-HIF1α−/−) in DRG neurons. The sciatic nerves were immunostained for the respective redoxins and counterstained with hematoxylin. The redoxin immunoreactivity was quantified with ImageJ. For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity [1] transformed to rainbow pseudocolors (Fig. 2). In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord (Fig. 7) and some redoxin examples of the thalamus (Fig. 8) are presented. Characteristics of the antibodies are listed in Table 1 along with some features of the redoxins.


Expression and regulation of redoxins at nociceptive signaling sites after sciatic nerve injury in mice.

Valek L, Kanngießer M, Tegeder I - Data Brief (2015)

Quantification of redoxin histology of the sciatic nerve with WCIF ImageJ. Mean pixel intensities were determined after substraction of background, RGB split into its channels and threshold settings based on negative control images using “auto” settings. The analysis did not differentiate between different types of cells and is a global readout for immunoreactivities in axonal fibers, Schwann cells and infiltrating immune cells. Pixel intensities were compared with one-way ANOVA for each redoxin separately. In case of significance, groups were mutually compared employing a Sidak correction of alpha, which was set at 0.05 for all comparisons. Asterisks indicate significant differences versus the respective naive animals. The analysis was based on results of 3 animals per group.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660239&req=5

f0030: Quantification of redoxin histology of the sciatic nerve with WCIF ImageJ. Mean pixel intensities were determined after substraction of background, RGB split into its channels and threshold settings based on negative control images using “auto” settings. The analysis did not differentiate between different types of cells and is a global readout for immunoreactivities in axonal fibers, Schwann cells and infiltrating immune cells. Pixel intensities were compared with one-way ANOVA for each redoxin separately. In case of significance, groups were mutually compared employing a Sidak correction of alpha, which was set at 0.05 for all comparisons. Asterisks indicate significant differences versus the respective naive animals. The analysis was based on results of 3 animals per group.
Mentions: The present DiB paper shows immunohistochemistry of redoxins including peroxiredoxins (Prdx1–6), glutaredoxins (Glrx1, 2, 3, 5), thioredoxins (Txn1, 2) and thioredoxin reductases (Txnrd1, 2) in the DRGs Figs. 1 and 2), spinal cord (Fig. 1, Fig. 2, Fig. 7), sciatic nerve (Fig. 3, Fig. 4, Fig. 5, quantification Fig. 6) and thalamus (Fig. 8) in naïve mice and 7 days after Spared sciatic Nerve Injury (SNI) in control mice (Hif1α-flfl) and in mice with a specific deletion of hypoxia inducible factor 1 alpha (SNS-HIF1α−/−) in DRG neurons. The sciatic nerves were immunostained for the respective redoxins and counterstained with hematoxylin. The redoxin immunoreactivity was quantified with ImageJ. For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity [1] transformed to rainbow pseudocolors (Fig. 2). In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord (Fig. 7) and some redoxin examples of the thalamus (Fig. 8) are presented. Characteristics of the antibodies are listed in Table 1 along with some features of the redoxins.

Bottom Line: The redoxin immunoreactivity was quantified with ImageJ.For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity transformed to rainbow pseudocolors.In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord and some redoxin examples of the thalamus are presented.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Pharmacology/ZAFES, Goethe-University Hospital, Frankfurt, Germany.

ABSTRACT
Injury of the sciatic nerve results in regulations of pro- and anti-oxidative enzymes at sites of nociceptive signaling including the injured nerve, dorsal root ganglia (DRGs), dorsal horn of the spinal cord, thalamus and somatosensory cortex (Valek et al., 2015) [1]. The present DiB paper shows immunohistochemistry of redoxins including peroxiredoxins (Prdx1-6), glutaredoxins (Glrx1, 2, 3, 5), thioredoxins (Txn1, 2) and thioredoxin reductases (Txnrd1, 2) in the DRGs, spinal cord and sciatic nerve and thalamus in naïve mice and 7 days after Spared sciatic Nerve Injury (SNI) in control mice (Hif1α-flfl) and in mice with a specific deletion of hypoxia inducible factor 1 alpha (SNS-HIF1α(-/-)) in DRG neurons. The sciatic nerves were immunostained for the respective redoxins and counterstained with hematoxylin. The redoxin immunoreactivity was quantified with ImageJ. For the DRGs and spinal cord the data show the quantitative assessment of the intensity of redoxin immunoreactivity transformed to rainbow pseudocolors. In addition, some redoxin examples of the ipsi and contralateral dorsal and ventral horns of the lumbar spinal cord and some redoxin examples of the thalamus are presented.

No MeSH data available.


Related in: MedlinePlus