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Virgibacillus senegalensis sp. nov., a new moderately halophilic bacterium isolated from human gut.

Seck E, Rathored J, Khelaifia S, Croce O, Robert C, Couderc C, Di Pinto F, Sokhna C, Raoult D, Lagier JC - New Microbes New Infect (2015)

Bottom Line: It is an aerobic, Gram positive, moderately halophilic, motile bipolar flagellum isolated from a healthy Senegalese man.Here we describe the genomic and phenotypic characteristics of this isolate.The 3 755 098 bp long genome (one chromosome, no plasmid) exhibits a G + C content of 42.9% and contains 3738 protein-coding and 95 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UM 63, CNRS 7278, IRD 198, Inserm 1095, Institut Hospitalo-Universitaire Méditerranée-Infection, Faculté de médecine, Aix-Marseille Université, Marseille, France.

ABSTRACT
Virgibacillus senegalensis SK-1(T) (= CSUR P1101 = DSM 28585) is the type strain of V. senegalensis sp. nov. It is an aerobic, Gram positive, moderately halophilic, motile bipolar flagellum isolated from a healthy Senegalese man. Here we describe the genomic and phenotypic characteristics of this isolate. The 3 755 098 bp long genome (one chromosome, no plasmid) exhibits a G + C content of 42.9% and contains 3738 protein-coding and 95 RNA genes.

No MeSH data available.


Related in: MedlinePlus

Gel view comparing Virgibacillus senegalensis sp. nov. SK-1Tto members of family Virgibacillus and Oceanobacillus. Gel view displays raw spectra of all loaded spectrum files arranged in pseudo-gel-like look. X-axis records m/z value. Left y-axis displays running spectrum number originating from subsequent spectra loading. Peak intensity is expressed by greyscale scheme code. Color bar and right y-axis indicate relation between color peak; peak intensity expressed in arbitrary units. Displayed species are indicated at left.
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fig2: Gel view comparing Virgibacillus senegalensis sp. nov. SK-1Tto members of family Virgibacillus and Oceanobacillus. Gel view displays raw spectra of all loaded spectrum files arranged in pseudo-gel-like look. X-axis records m/z value. Left y-axis displays running spectrum number originating from subsequent spectra loading. Peak intensity is expressed by greyscale scheme code. Color bar and right y-axis indicate relation between color peak; peak intensity expressed in arbitrary units. Displayed species are indicated at left.

Mentions: Strain SK-1T was isolated in February 2014 (Table 1) by aerobic culture on a homemade culture medium at 37°C after 48 hours. No significant MALDI-TOF result for the strain SK-1T against our MALDI-TOF database was obtained, suggesting that our isolate was not a previously known species. We added the specter of SK-1T strain to our database (Fig. 1). The gel view allowed us to observe the spectral differences with other members of Virgibacillus genus (Fig. 2). PCR-based identification of the 16S rRNA of our new isolate (GenBank accession number LK021111) yielded 96.3% 16S rRNA sequence similarity with the reference Virgibacillus kekensis (GenBank accession number NR042744), the phylogenetically closest validated Virgibacillus species (Fig. 3).


Virgibacillus senegalensis sp. nov., a new moderately halophilic bacterium isolated from human gut.

Seck E, Rathored J, Khelaifia S, Croce O, Robert C, Couderc C, Di Pinto F, Sokhna C, Raoult D, Lagier JC - New Microbes New Infect (2015)

Gel view comparing Virgibacillus senegalensis sp. nov. SK-1Tto members of family Virgibacillus and Oceanobacillus. Gel view displays raw spectra of all loaded spectrum files arranged in pseudo-gel-like look. X-axis records m/z value. Left y-axis displays running spectrum number originating from subsequent spectra loading. Peak intensity is expressed by greyscale scheme code. Color bar and right y-axis indicate relation between color peak; peak intensity expressed in arbitrary units. Displayed species are indicated at left.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660226&req=5

fig2: Gel view comparing Virgibacillus senegalensis sp. nov. SK-1Tto members of family Virgibacillus and Oceanobacillus. Gel view displays raw spectra of all loaded spectrum files arranged in pseudo-gel-like look. X-axis records m/z value. Left y-axis displays running spectrum number originating from subsequent spectra loading. Peak intensity is expressed by greyscale scheme code. Color bar and right y-axis indicate relation between color peak; peak intensity expressed in arbitrary units. Displayed species are indicated at left.
Mentions: Strain SK-1T was isolated in February 2014 (Table 1) by aerobic culture on a homemade culture medium at 37°C after 48 hours. No significant MALDI-TOF result for the strain SK-1T against our MALDI-TOF database was obtained, suggesting that our isolate was not a previously known species. We added the specter of SK-1T strain to our database (Fig. 1). The gel view allowed us to observe the spectral differences with other members of Virgibacillus genus (Fig. 2). PCR-based identification of the 16S rRNA of our new isolate (GenBank accession number LK021111) yielded 96.3% 16S rRNA sequence similarity with the reference Virgibacillus kekensis (GenBank accession number NR042744), the phylogenetically closest validated Virgibacillus species (Fig. 3).

Bottom Line: It is an aerobic, Gram positive, moderately halophilic, motile bipolar flagellum isolated from a healthy Senegalese man.Here we describe the genomic and phenotypic characteristics of this isolate.The 3 755 098 bp long genome (one chromosome, no plasmid) exhibits a G + C content of 42.9% and contains 3738 protein-coding and 95 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UM 63, CNRS 7278, IRD 198, Inserm 1095, Institut Hospitalo-Universitaire Méditerranée-Infection, Faculté de médecine, Aix-Marseille Université, Marseille, France.

ABSTRACT
Virgibacillus senegalensis SK-1(T) (= CSUR P1101 = DSM 28585) is the type strain of V. senegalensis sp. nov. It is an aerobic, Gram positive, moderately halophilic, motile bipolar flagellum isolated from a healthy Senegalese man. Here we describe the genomic and phenotypic characteristics of this isolate. The 3 755 098 bp long genome (one chromosome, no plasmid) exhibits a G + C content of 42.9% and contains 3738 protein-coding and 95 RNA genes.

No MeSH data available.


Related in: MedlinePlus