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Genome sequence and description of Mannheimia massilioguelmaensis sp. nov.

Hadjadj L, Bentorki AA, Michelle C, Amoura K, Djahoudi A, Rolain JM - New Microbes New Infect (2015)

Bottom Line: Mannheimia massilioguelmaensis is a Gram-negative, facultative anaerobic rod, member of the family Pasteurellaceae.Here we describe this organism, together with the complete genome sequence and annotation.The 2 186 813 bp long genome contains 2048 protein-coding and 55 RNA genes, including eight rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE), UMR CNRS, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.

ABSTRACT
Strain MG13(T) sp. nov. is the type strain of Mannheimia massilioguelmaensis, a new species within the genus Mannheimia. This strain was isolated from the exudate of a skin lesion of an Algerian man. Mannheimia massilioguelmaensis is a Gram-negative, facultative anaerobic rod, member of the family Pasteurellaceae. Here we describe this organism, together with the complete genome sequence and annotation. The 2 186 813 bp long genome contains 2048 protein-coding and 55 RNA genes, including eight rRNA genes.

No MeSH data available.


Related in: MedlinePlus

Reference mass spectrum from Mannheimia massilioguelmaensis strain MG13T. Spectra from 12 individual colonies were compared and reference spectrum generated.
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fig3: Reference mass spectrum from Mannheimia massilioguelmaensis strain MG13T. Spectra from 12 individual colonies were compared and reference spectrum generated.

Mentions: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) protein analysis was performed as previously described [9] using a Microflex spectrometer (Bruker). Twelve distinct deposits were done for strain MG13T from 12 isolated colonies. The 12 MG13T spectra were imported into the MALDI BioTyper 2.0 software (Bruker) and analysed by standard pattern matching (with default parameter settings) against the main spectra of 4108 bacteria, including seven spectra from four Mannheimia species, used as reference data, in the BioTyper database. A score enabled the identification (or not) from the tested species: a score of >2 with a validated species enabled the identification at the species level; a score of >1.7 but <2 enabled the identification at the genus level; and a score of <1.7 did not enable any identification. No significant MALDI-TOF score was obtained for strain MG13T against the Bruker database, thus suggesting that our isolate was a new species. We incremented our database with the spectrum from strain MG13T (Fig. 3).


Genome sequence and description of Mannheimia massilioguelmaensis sp. nov.

Hadjadj L, Bentorki AA, Michelle C, Amoura K, Djahoudi A, Rolain JM - New Microbes New Infect (2015)

Reference mass spectrum from Mannheimia massilioguelmaensis strain MG13T. Spectra from 12 individual colonies were compared and reference spectrum generated.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660222&req=5

fig3: Reference mass spectrum from Mannheimia massilioguelmaensis strain MG13T. Spectra from 12 individual colonies were compared and reference spectrum generated.
Mentions: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) protein analysis was performed as previously described [9] using a Microflex spectrometer (Bruker). Twelve distinct deposits were done for strain MG13T from 12 isolated colonies. The 12 MG13T spectra were imported into the MALDI BioTyper 2.0 software (Bruker) and analysed by standard pattern matching (with default parameter settings) against the main spectra of 4108 bacteria, including seven spectra from four Mannheimia species, used as reference data, in the BioTyper database. A score enabled the identification (or not) from the tested species: a score of >2 with a validated species enabled the identification at the species level; a score of >1.7 but <2 enabled the identification at the genus level; and a score of <1.7 did not enable any identification. No significant MALDI-TOF score was obtained for strain MG13T against the Bruker database, thus suggesting that our isolate was a new species. We incremented our database with the spectrum from strain MG13T (Fig. 3).

Bottom Line: Mannheimia massilioguelmaensis is a Gram-negative, facultative anaerobic rod, member of the family Pasteurellaceae.Here we describe this organism, together with the complete genome sequence and annotation.The 2 186 813 bp long genome contains 2048 protein-coding and 55 RNA genes, including eight rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE), UMR CNRS, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.

ABSTRACT
Strain MG13(T) sp. nov. is the type strain of Mannheimia massilioguelmaensis, a new species within the genus Mannheimia. This strain was isolated from the exudate of a skin lesion of an Algerian man. Mannheimia massilioguelmaensis is a Gram-negative, facultative anaerobic rod, member of the family Pasteurellaceae. Here we describe this organism, together with the complete genome sequence and annotation. The 2 186 813 bp long genome contains 2048 protein-coding and 55 RNA genes, including eight rRNA genes.

No MeSH data available.


Related in: MedlinePlus