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Genome sequence and description of Mannheimia massilioguelmaensis sp. nov.

Hadjadj L, Bentorki AA, Michelle C, Amoura K, Djahoudi A, Rolain JM - New Microbes New Infect (2015)

Bottom Line: Mannheimia massilioguelmaensis is a Gram-negative, facultative anaerobic rod, member of the family Pasteurellaceae.Here we describe this organism, together with the complete genome sequence and annotation.The 2 186 813 bp long genome contains 2048 protein-coding and 55 RNA genes, including eight rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE), UMR CNRS, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.

ABSTRACT
Strain MG13(T) sp. nov. is the type strain of Mannheimia massilioguelmaensis, a new species within the genus Mannheimia. This strain was isolated from the exudate of a skin lesion of an Algerian man. Mannheimia massilioguelmaensis is a Gram-negative, facultative anaerobic rod, member of the family Pasteurellaceae. Here we describe this organism, together with the complete genome sequence and annotation. The 2 186 813 bp long genome contains 2048 protein-coding and 55 RNA genes, including eight rRNA genes.

No MeSH data available.


Related in: MedlinePlus

Gram staining of Mannheimia massilioguelmaensis strain MG13T.
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fig1: Gram staining of Mannheimia massilioguelmaensis strain MG13T.

Mentions: Different growth temperatures (25, 30, 37 and 45°C) were tested. Growth occurred between 25°C and 37°C, but optimal growth was observed at 37°C, 24 hours after inoculation. Colonies were smooth, greyish and approximately 1 mm in diameter on 5% sheep's blood–enriched agar (bioMérieux). Growth of the strain was tested in anaerobic and microaerophilic atmospheres using GasPak EZ Anaerobe Pouch (Becton Dickinson) and CampyGen Compact (Oxoid) systems, respectively, and in aerobic atmosphere, with or without 5% CO2. Growth was observed under aerobic (with and without CO2), microaerophilic and anaerobic conditions. Gram staining showed short Gram-negative rods unable to form spores (Fig. 1). A motility test was negative. The size of cells were determined by negative staining transmission electron microscopy on a Technai G20 Cryo device (FEI) at an operating voltage of 200 kV. The rods had a length ranging from 1.1 to 1.9 μm (mean 1.5 μm), a width ranging from 0.4 to 0.6 μm (mean 0.5 μm) and a diameter ranging from 0.4 to 0.8 μm (mean 0.6 μm) (Fig. 2).


Genome sequence and description of Mannheimia massilioguelmaensis sp. nov.

Hadjadj L, Bentorki AA, Michelle C, Amoura K, Djahoudi A, Rolain JM - New Microbes New Infect (2015)

Gram staining of Mannheimia massilioguelmaensis strain MG13T.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660222&req=5

fig1: Gram staining of Mannheimia massilioguelmaensis strain MG13T.
Mentions: Different growth temperatures (25, 30, 37 and 45°C) were tested. Growth occurred between 25°C and 37°C, but optimal growth was observed at 37°C, 24 hours after inoculation. Colonies were smooth, greyish and approximately 1 mm in diameter on 5% sheep's blood–enriched agar (bioMérieux). Growth of the strain was tested in anaerobic and microaerophilic atmospheres using GasPak EZ Anaerobe Pouch (Becton Dickinson) and CampyGen Compact (Oxoid) systems, respectively, and in aerobic atmosphere, with or without 5% CO2. Growth was observed under aerobic (with and without CO2), microaerophilic and anaerobic conditions. Gram staining showed short Gram-negative rods unable to form spores (Fig. 1). A motility test was negative. The size of cells were determined by negative staining transmission electron microscopy on a Technai G20 Cryo device (FEI) at an operating voltage of 200 kV. The rods had a length ranging from 1.1 to 1.9 μm (mean 1.5 μm), a width ranging from 0.4 to 0.6 μm (mean 0.5 μm) and a diameter ranging from 0.4 to 0.8 μm (mean 0.6 μm) (Fig. 2).

Bottom Line: Mannheimia massilioguelmaensis is a Gram-negative, facultative anaerobic rod, member of the family Pasteurellaceae.Here we describe this organism, together with the complete genome sequence and annotation.The 2 186 813 bp long genome contains 2048 protein-coding and 55 RNA genes, including eight rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE), UMR CNRS, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.

ABSTRACT
Strain MG13(T) sp. nov. is the type strain of Mannheimia massilioguelmaensis, a new species within the genus Mannheimia. This strain was isolated from the exudate of a skin lesion of an Algerian man. Mannheimia massilioguelmaensis is a Gram-negative, facultative anaerobic rod, member of the family Pasteurellaceae. Here we describe this organism, together with the complete genome sequence and annotation. The 2 186 813 bp long genome contains 2048 protein-coding and 55 RNA genes, including eight rRNA genes.

No MeSH data available.


Related in: MedlinePlus