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Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide.

Pawar HA, Lalitha KG - Int J Biomater (2015)

Bottom Line: The average molecular weight of purified S. tora gum was found to be 198 kDa by intrinsic viscosity method.The results indicated that viscosity of gum solution increases with increase in temperature.It has been concluded that extracted polysaccharide can be used as pharmaceutical excipient in terms of flow behavior, microbial properties, and stability.

View Article: PubMed Central - PubMed

Affiliation: Department of Quality Assurance, Dr. L. H. Hiranandani College of Pharmacy, Ulhasnagar, Maharashtra 421003, India.

ABSTRACT
The objective of the present work was extraction of polysaccharide from Senna tora L. seed and its characterization as a pharmaceutical excipient. Polysaccharide extraction was based on mechanical separation of the endosperm of seeds of Senna tora, water dissolution, centrifugation, and precipitation with acetone. Standard procedures were used to study the viscosity, micromeritic properties, and microbial bioburden. Accelerated stability study was carried out on isolated polysaccharide for six months at 40°C/75 RH as per ICH guidelines. The gum obtained from S. tora seeds was an amorphous free flowing odourless powder with dull brown colour (yield = 35% w/w). The bulk density, tapped density, and angle of repose data reveal that S. tora gum possesses good flow property. The intrinsic viscosity obtained was 1.568 dL/g. The average molecular weight of purified S. tora gum was found to be 198 kDa by intrinsic viscosity method. The results indicated that viscosity of gum solution increases with increase in temperature. FTIR study revealed the absence of degradation or decomposition of polysaccharide at accelerated stability conditions for six months. It has been concluded that extracted polysaccharide can be used as pharmaceutical excipient in terms of flow behavior, microbial properties, and stability.

No MeSH data available.


Related in: MedlinePlus

Photograph of gum powder taken (a) under high magnification microscope and (b) using digital camera.
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fig1: Photograph of gum powder taken (a) under high magnification microscope and (b) using digital camera.

Mentions: The gum obtained from S. tora seeds was an amorphous free flowing odourless powder with dull brown colour (yield = 35% w/w). The photograph of the gum powder was taken under high intensity electron light microscope and using digital camera as depicted in Figures 1(a) and 1(b).


Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide.

Pawar HA, Lalitha KG - Int J Biomater (2015)

Photograph of gum powder taken (a) under high magnification microscope and (b) using digital camera.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4660029&req=5

fig1: Photograph of gum powder taken (a) under high magnification microscope and (b) using digital camera.
Mentions: The gum obtained from S. tora seeds was an amorphous free flowing odourless powder with dull brown colour (yield = 35% w/w). The photograph of the gum powder was taken under high intensity electron light microscope and using digital camera as depicted in Figures 1(a) and 1(b).

Bottom Line: The average molecular weight of purified S. tora gum was found to be 198 kDa by intrinsic viscosity method.The results indicated that viscosity of gum solution increases with increase in temperature.It has been concluded that extracted polysaccharide can be used as pharmaceutical excipient in terms of flow behavior, microbial properties, and stability.

View Article: PubMed Central - PubMed

Affiliation: Department of Quality Assurance, Dr. L. H. Hiranandani College of Pharmacy, Ulhasnagar, Maharashtra 421003, India.

ABSTRACT
The objective of the present work was extraction of polysaccharide from Senna tora L. seed and its characterization as a pharmaceutical excipient. Polysaccharide extraction was based on mechanical separation of the endosperm of seeds of Senna tora, water dissolution, centrifugation, and precipitation with acetone. Standard procedures were used to study the viscosity, micromeritic properties, and microbial bioburden. Accelerated stability study was carried out on isolated polysaccharide for six months at 40°C/75 RH as per ICH guidelines. The gum obtained from S. tora seeds was an amorphous free flowing odourless powder with dull brown colour (yield = 35% w/w). The bulk density, tapped density, and angle of repose data reveal that S. tora gum possesses good flow property. The intrinsic viscosity obtained was 1.568 dL/g. The average molecular weight of purified S. tora gum was found to be 198 kDa by intrinsic viscosity method. The results indicated that viscosity of gum solution increases with increase in temperature. FTIR study revealed the absence of degradation or decomposition of polysaccharide at accelerated stability conditions for six months. It has been concluded that extracted polysaccharide can be used as pharmaceutical excipient in terms of flow behavior, microbial properties, and stability.

No MeSH data available.


Related in: MedlinePlus