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Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress.

Zhou Y, Zhao L, Zhang Z, Lu X - Biomed Res Int (2015)

Bottom Line: MED significantly increased plasma levels of homocysteine (Hcy) and ACE.Moreover, MED resulted in oxidative stress as evidenced by significant increase of TBARS level and decrease of superoxide dismutase and catalase activities.The data provide novel insights into the mechanism of high Hcy-associated hypertension and the therapeutic efficiency of enalapril.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China ; Intervention Therapy Department of Vascular Disease, Chinese Medicine Hospital of Shaanxi Province, Xi'an 710003, China.

ABSTRACT
In the present study, we investigated the effect of methionine-enriched diet (MED) on blood pressure in rats and examined the protective effect of enalapril, a widely used angiotensin converting enzyme inhibitors (ACEi) class antihypertensive drug. The results showed that MED induced significant increase of SBP and Ang II-induced contractile response in aortae of rats. MED significantly increased plasma levels of homocysteine (Hcy) and ACE. In addition, MED increased the phosphorylation of protein kinase R-like endoplasmic reticulum kinase (PERK) and eukaryotic initiation factor 2 (eIF2α) and expression of activating transcription factor 3 (ATF3) and ATF6 in aortae of rats, indicating the occurrence of endoplasmic reticulum (ER) stress. Moreover, MED resulted in oxidative stress as evidenced by significant increase of TBARS level and decrease of superoxide dismutase and catalase activities. Administration of enalapril could effectively inhibit these pathological changes induced by MED in rats. These results demonstrated that ACE-mediated ER stress and oxidative stress played an important role in high Hcy-induced hypertension and MED may exert a positive loop between the activation of ACE and accumulation of Hcy, aggravating the pathological condition of hypertension. The data provide novel insights into the mechanism of high Hcy-associated hypertension and the therapeutic efficiency of enalapril.

No MeSH data available.


Related in: MedlinePlus

Effect of MED and enalapril on the levels of plasma Hcy in rats. Rats were fed SD or MED from 30th to 90th postnatal day with or without enalapril treatment from 60th to 90th postnatal day. After the experiment, plasma levels of Hcy were measured. ∗p < 0.05, compared with that of SD rats. #p < 0.05, compared with that of MED rats.
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fig3: Effect of MED and enalapril on the levels of plasma Hcy in rats. Rats were fed SD or MED from 30th to 90th postnatal day with or without enalapril treatment from 60th to 90th postnatal day. After the experiment, plasma levels of Hcy were measured. ∗p < 0.05, compared with that of SD rats. #p < 0.05, compared with that of MED rats.

Mentions: Plasma Hcy levels were examined in the study. In Figure 3, we showed that MED increased the plasma levels of Hcy to more than three times that of SD rats. In addition, the treatment of enalapril significantly inhibited the increase of plasma levels of Hcy induced by MED.


Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress.

Zhou Y, Zhao L, Zhang Z, Lu X - Biomed Res Int (2015)

Effect of MED and enalapril on the levels of plasma Hcy in rats. Rats were fed SD or MED from 30th to 90th postnatal day with or without enalapril treatment from 60th to 90th postnatal day. After the experiment, plasma levels of Hcy were measured. ∗p < 0.05, compared with that of SD rats. #p < 0.05, compared with that of MED rats.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660008&req=5

fig3: Effect of MED and enalapril on the levels of plasma Hcy in rats. Rats were fed SD or MED from 30th to 90th postnatal day with or without enalapril treatment from 60th to 90th postnatal day. After the experiment, plasma levels of Hcy were measured. ∗p < 0.05, compared with that of SD rats. #p < 0.05, compared with that of MED rats.
Mentions: Plasma Hcy levels were examined in the study. In Figure 3, we showed that MED increased the plasma levels of Hcy to more than three times that of SD rats. In addition, the treatment of enalapril significantly inhibited the increase of plasma levels of Hcy induced by MED.

Bottom Line: MED significantly increased plasma levels of homocysteine (Hcy) and ACE.Moreover, MED resulted in oxidative stress as evidenced by significant increase of TBARS level and decrease of superoxide dismutase and catalase activities.The data provide novel insights into the mechanism of high Hcy-associated hypertension and the therapeutic efficiency of enalapril.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China ; Intervention Therapy Department of Vascular Disease, Chinese Medicine Hospital of Shaanxi Province, Xi'an 710003, China.

ABSTRACT
In the present study, we investigated the effect of methionine-enriched diet (MED) on blood pressure in rats and examined the protective effect of enalapril, a widely used angiotensin converting enzyme inhibitors (ACEi) class antihypertensive drug. The results showed that MED induced significant increase of SBP and Ang II-induced contractile response in aortae of rats. MED significantly increased plasma levels of homocysteine (Hcy) and ACE. In addition, MED increased the phosphorylation of protein kinase R-like endoplasmic reticulum kinase (PERK) and eukaryotic initiation factor 2 (eIF2α) and expression of activating transcription factor 3 (ATF3) and ATF6 in aortae of rats, indicating the occurrence of endoplasmic reticulum (ER) stress. Moreover, MED resulted in oxidative stress as evidenced by significant increase of TBARS level and decrease of superoxide dismutase and catalase activities. Administration of enalapril could effectively inhibit these pathological changes induced by MED in rats. These results demonstrated that ACE-mediated ER stress and oxidative stress played an important role in high Hcy-induced hypertension and MED may exert a positive loop between the activation of ACE and accumulation of Hcy, aggravating the pathological condition of hypertension. The data provide novel insights into the mechanism of high Hcy-associated hypertension and the therapeutic efficiency of enalapril.

No MeSH data available.


Related in: MedlinePlus