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Ecuador Paraiso Escondido Virus, a New Flavivirus Isolated from New World Sand Flies in Ecuador, Is the First Representative of a Novel Clade in the Genus Flavivirus.

Alkan C, Zapata S, Bichaud L, Moureau G, Lemey P, Firth AE, Gritsun TS, Gould EA, de Lamballerie X, Depaquit J, Charrel RN - J. Virol. (2015)

Bottom Line: This surprising result, which appears to eliminate an association with vertebrate hosts in the life cycle of EPEV, is discussed in the context of the evolutionary origins of EPEV in the New World.EPEV constitutes a novel clade according to current knowledge of the flaviviruses.In light of this new discovery, the New World origin of EPEV is discussed together with that of the other flaviviruses.

View Article: PubMed Central - PubMed

Affiliation: Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranée Infection, APHM Public Hospitals of Marseille, Marseille, France cgdmalkan@gmail.com remi.charrel@univ-amu.fr.

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Conserved motifs in the polyprotein of EPEV. The numbers 1 to 4 indicate the conserved enzymatic motifs in the proteins encoded by the NS3 and NS5 genes. Slashes, gaps; residues in yellow, nonconserved positions.
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Figure 2: Conserved motifs in the polyprotein of EPEV. The numbers 1 to 4 indicate the conserved enzymatic motifs in the proteins encoded by the NS3 and NS5 genes. Slashes, gaps; residues in yellow, nonconserved positions.

Mentions: The N-terminal sequence of the flavivirus NS3 protein contains conserved regions (boxes 1 to 4) that have significant similarity to serine proteases and belong to the trypsin superfamily (69, 70). This protease activity was reported to be required for the polyprotein processing of flaviviruses. On the other hand, the C-terminal sequence contains conserved regions (motifs I, IA, and II to VI) which are similar to RNA helicases of the DEAD family (71). These motifs are shown in Fig. 2 for EPEV. Compared with the sequences of other flaviviruses, these patterns are highly conserved in the EPEV genome. The EPEV NS5 protein contains the conserved sequences (motifs A to D) associated with RNA-dependent RNA polymerase activity (72) and also the Gly-Asp-Asp sequence of motif C, which is the active site of the polymerase. In the N-terminal domain, two conserved motifs, viz., motifs 1 and 2, are similar to methyltransferases (Fig. 2).


Ecuador Paraiso Escondido Virus, a New Flavivirus Isolated from New World Sand Flies in Ecuador, Is the First Representative of a Novel Clade in the Genus Flavivirus.

Alkan C, Zapata S, Bichaud L, Moureau G, Lemey P, Firth AE, Gritsun TS, Gould EA, de Lamballerie X, Depaquit J, Charrel RN - J. Virol. (2015)

Conserved motifs in the polyprotein of EPEV. The numbers 1 to 4 indicate the conserved enzymatic motifs in the proteins encoded by the NS3 and NS5 genes. Slashes, gaps; residues in yellow, nonconserved positions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4645344&req=5

Figure 2: Conserved motifs in the polyprotein of EPEV. The numbers 1 to 4 indicate the conserved enzymatic motifs in the proteins encoded by the NS3 and NS5 genes. Slashes, gaps; residues in yellow, nonconserved positions.
Mentions: The N-terminal sequence of the flavivirus NS3 protein contains conserved regions (boxes 1 to 4) that have significant similarity to serine proteases and belong to the trypsin superfamily (69, 70). This protease activity was reported to be required for the polyprotein processing of flaviviruses. On the other hand, the C-terminal sequence contains conserved regions (motifs I, IA, and II to VI) which are similar to RNA helicases of the DEAD family (71). These motifs are shown in Fig. 2 for EPEV. Compared with the sequences of other flaviviruses, these patterns are highly conserved in the EPEV genome. The EPEV NS5 protein contains the conserved sequences (motifs A to D) associated with RNA-dependent RNA polymerase activity (72) and also the Gly-Asp-Asp sequence of motif C, which is the active site of the polymerase. In the N-terminal domain, two conserved motifs, viz., motifs 1 and 2, are similar to methyltransferases (Fig. 2).

Bottom Line: This surprising result, which appears to eliminate an association with vertebrate hosts in the life cycle of EPEV, is discussed in the context of the evolutionary origins of EPEV in the New World.EPEV constitutes a novel clade according to current knowledge of the flaviviruses.In light of this new discovery, the New World origin of EPEV is discussed together with that of the other flaviviruses.

View Article: PubMed Central - PubMed

Affiliation: Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranée Infection, APHM Public Hospitals of Marseille, Marseille, France cgdmalkan@gmail.com remi.charrel@univ-amu.fr.

Show MeSH
Related in: MedlinePlus