Characterization of a Novel Hepadnavirus in the White Sucker (Catostomus commersonii) from the Great Lakes Region of the United States.
Bottom Line: Phylogenetic analyses confirmed WSHBV as distinct from previously documented hepadnaviruses.These results are not sufficient to draw inferences regarding the association of WSHBV and carcinogenesis in white sucker.This may also offer another model system for mechanistic research.
Affiliation: West Virginia University, School of Natural Resources, Morgantown, West Virginia, USA U.S. Geological Survey, Leetown Science Center, Kearneysville, West Virginia, USA.Show MeSH
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Mentions: We collected liver tissue from wild-caught, white sucker (n = 169) inhabiting five rivers in the Great Lakes region (Fig. 1; see also Table S1 in the supplemental material). These samples were preserved in RNAlater (Life Technologies, Grand Island, NY) for a transcriptome assembly project (PRJNA282680) and quantitative gene expression analyses. A liver sample from a white sucker collected from Michael Brook near Carmel, NY, was included for this purpose as well. Tissue was not collected for DNA applications from these fish. In an attempt to collect samples suitable for DNA analysis, we collected white sucker (n = 20) from the Root River (Racine, WI) in the spring of 2014. Liver tissue was excised and stored in 90% ethanol for subsequent DNA extraction. All fish were euthanized with a lethal dose of tricane methanesulfonate (MS-222, Finquel; Argent Laboratories, Redmond, WA) according to approved Animal and Care Safety protocols (U.S. Geological Survey, Leetown Science Center, Kearneysville, WV). Prior to necropsy, blood was collected using heparinized syringes, transferred to heparinized Vacutainers, and stored on wet ice until centrifuged. Plasma samples were stored at −80°C until analyzed. Additional liver tissue was preserved in Z-fix (Anatech Ltd., Battle Creek, MI) from all individuals for subsequent histopathological assessment.
Affiliation: West Virginia University, School of Natural Resources, Morgantown, West Virginia, USA U.S. Geological Survey, Leetown Science Center, Kearneysville, West Virginia, USA.