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Regulation of formation of volatile compounds of tea (Camellia sinensis) leaves by single light wavelength.

Fu X, Chen Y, Mei X, Katsuno T, Kobayashi E, Dong F, Watanabe N, Yang Z - Sci Rep (2015)

Bottom Line: Less attention has been paid to effect of light wavelength on formation of plant metabolites.Furthermore, blue and red lights significantly up-regulated the expression levels of 9/13-lipoxygenases involved in VFADs formation, phenylalanine ammonialyase involved in VPBs formation, and terpene synthases involved in VTs formation.These results suggest that blue and red lights can be promising technology for remodeling the aroma of preharvest tea leaves.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.

ABSTRACT
Regulation of plant growth and development by light wavelength has been extensively studied. Less attention has been paid to effect of light wavelength on formation of plant metabolites. The objective of this study was to investigate whether formation of volatiles in preharvest and postharvest tea (Camellia sinensis) leaves can be regulated by light wavelength. In the present study, in contrast to the natural light or dark treatment, blue light (470 nm) and red light (660 nm) significantly increased most endogenous volatiles including volatile fatty acid derivatives (VFADs), volatile phenylpropanoids/benzenoids (VPBs), and volatile terpenes (VTs) in the preharvest tea leaves. Furthermore, blue and red lights significantly up-regulated the expression levels of 9/13-lipoxygenases involved in VFADs formation, phenylalanine ammonialyase involved in VPBs formation, and terpene synthases involved in VTs formation. Single light wavelength had less remarkable influences on formation of volatiles in the postharvest leaves compared with the preharvest leaves. These results suggest that blue and red lights can be promising technology for remodeling the aroma of preharvest tea leaves. Furthermore, our study provided evidence that light wavelength can activate the expression of key genes involved in formation of plant volatiles for the first time.

No MeSH data available.


Effects of blue light and red light on the pathway leading from linolenic acid to volatile fatty acids in preharvest tea leaves.D, dark treatment. B, blue light treatment. R, red light treatment. N.D., not detected. I.S., internal standard. LOX, 9/13-lipoxygenases. All treatments on preharvest tea leaves were carried out for 3 days. The relative content (%) of metabolites was calculated based on the dark treatment (100%) as a control. The relative level of genes was calculated based on the dark treatment (1) as a control. Different means with different letters are significantly different from each other (p ≤ 0.05).
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f3: Effects of blue light and red light on the pathway leading from linolenic acid to volatile fatty acids in preharvest tea leaves.D, dark treatment. B, blue light treatment. R, red light treatment. N.D., not detected. I.S., internal standard. LOX, 9/13-lipoxygenases. All treatments on preharvest tea leaves were carried out for 3 days. The relative content (%) of metabolites was calculated based on the dark treatment (100%) as a control. The relative level of genes was calculated based on the dark treatment (1) as a control. Different means with different letters are significantly different from each other (p ≤ 0.05).

Mentions: The preharvest tea leaves contained the three classes of major volatiles including volatile fatty acid derivatives (VFADs), VPBs, and volatile terpenes (VTs). In our preliminary experiment, LED was applied to treat preharvest tea leaves from the 1st leaf stage to the 4th leaf stage (14 days), and the results showed that in contrast to dark treatment as a control, blue light and red light increased the contents of VFADs and VPBs, whereas near-infrared light had less effect on tea volatiles. In addition, VTs decreased or were not affected by the long term (14 days) LED treatments (Fig. S1, Supplementary information). In the present study, 3 days of blue light and red light treatments on the 4th leaf stage of preharvest tea leaves increased most endogenous volatiles including VFADs, VPBs, and VTs compared with dark treatment (Figs 1, 2, 3).


Regulation of formation of volatile compounds of tea (Camellia sinensis) leaves by single light wavelength.

Fu X, Chen Y, Mei X, Katsuno T, Kobayashi E, Dong F, Watanabe N, Yang Z - Sci Rep (2015)

Effects of blue light and red light on the pathway leading from linolenic acid to volatile fatty acids in preharvest tea leaves.D, dark treatment. B, blue light treatment. R, red light treatment. N.D., not detected. I.S., internal standard. LOX, 9/13-lipoxygenases. All treatments on preharvest tea leaves were carried out for 3 days. The relative content (%) of metabolites was calculated based on the dark treatment (100%) as a control. The relative level of genes was calculated based on the dark treatment (1) as a control. Different means with different letters are significantly different from each other (p ≤ 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4645219&req=5

f3: Effects of blue light and red light on the pathway leading from linolenic acid to volatile fatty acids in preharvest tea leaves.D, dark treatment. B, blue light treatment. R, red light treatment. N.D., not detected. I.S., internal standard. LOX, 9/13-lipoxygenases. All treatments on preharvest tea leaves were carried out for 3 days. The relative content (%) of metabolites was calculated based on the dark treatment (100%) as a control. The relative level of genes was calculated based on the dark treatment (1) as a control. Different means with different letters are significantly different from each other (p ≤ 0.05).
Mentions: The preharvest tea leaves contained the three classes of major volatiles including volatile fatty acid derivatives (VFADs), VPBs, and volatile terpenes (VTs). In our preliminary experiment, LED was applied to treat preharvest tea leaves from the 1st leaf stage to the 4th leaf stage (14 days), and the results showed that in contrast to dark treatment as a control, blue light and red light increased the contents of VFADs and VPBs, whereas near-infrared light had less effect on tea volatiles. In addition, VTs decreased or were not affected by the long term (14 days) LED treatments (Fig. S1, Supplementary information). In the present study, 3 days of blue light and red light treatments on the 4th leaf stage of preharvest tea leaves increased most endogenous volatiles including VFADs, VPBs, and VTs compared with dark treatment (Figs 1, 2, 3).

Bottom Line: Less attention has been paid to effect of light wavelength on formation of plant metabolites.Furthermore, blue and red lights significantly up-regulated the expression levels of 9/13-lipoxygenases involved in VFADs formation, phenylalanine ammonialyase involved in VPBs formation, and terpene synthases involved in VTs formation.These results suggest that blue and red lights can be promising technology for remodeling the aroma of preharvest tea leaves.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.

ABSTRACT
Regulation of plant growth and development by light wavelength has been extensively studied. Less attention has been paid to effect of light wavelength on formation of plant metabolites. The objective of this study was to investigate whether formation of volatiles in preharvest and postharvest tea (Camellia sinensis) leaves can be regulated by light wavelength. In the present study, in contrast to the natural light or dark treatment, blue light (470 nm) and red light (660 nm) significantly increased most endogenous volatiles including volatile fatty acid derivatives (VFADs), volatile phenylpropanoids/benzenoids (VPBs), and volatile terpenes (VTs) in the preharvest tea leaves. Furthermore, blue and red lights significantly up-regulated the expression levels of 9/13-lipoxygenases involved in VFADs formation, phenylalanine ammonialyase involved in VPBs formation, and terpene synthases involved in VTs formation. Single light wavelength had less remarkable influences on formation of volatiles in the postharvest leaves compared with the preharvest leaves. These results suggest that blue and red lights can be promising technology for remodeling the aroma of preharvest tea leaves. Furthermore, our study provided evidence that light wavelength can activate the expression of key genes involved in formation of plant volatiles for the first time.

No MeSH data available.