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Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum.

Tilly AK, Thiede J, Metwally N, Lubiana P, Bachmann A, Roeder T, Rockliffe N, Lorenzen S, Tannich E, Gutsmann T, Bruchhaus I - Sci Rep (2015)

Bottom Line: In addition, there was a greater reduction in the cytoadhesion of IEs to various endothelial receptors in the presence of AlbuMAX than in the presence of human serum.Surprisingly, cytoadhesion did not correlate with the presence or absence of knobs.Greater numbers of the variant surface antigen families RIFIN, STEVOR, and PfMC-2TM were found at the IE membrane when cultivated in the presence of AlbuMAX.

View Article: PubMed Central - PubMed

Affiliation: Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany.

ABSTRACT
In vitro cultivation of Plasmodium falciparum is critical for studying the biology of this parasite. However, it is likely that different in vitro cultivation conditions influence various aspects of the parasite's life cycle. In the present study two P. falciparum isolates were cultivated using the two most common methods, in which AlbuMAX or human serum as additives are used, and the results were compared. The type of cultivation influenced the knob structure of P. falciparum-infected erythrocytes (IEs). IEs cultivated with AlbuMAX had fewer knobs than those cultivated with human serum. Furthermore, knob size varied between isolates and is also depended on the culture medium. In addition, there was a greater reduction in the cytoadhesion of IEs to various endothelial receptors in the presence of AlbuMAX than in the presence of human serum. Surprisingly, cytoadhesion did not correlate with the presence or absence of knobs. Greater numbers of the variant surface antigen families RIFIN, STEVOR, and PfMC-2TM were found at the IE membrane when cultivated in the presence of AlbuMAX. Moreover, the type of cultivation had a marked influence on the transcriptome profile. Compared with cultivation with human serum, cultivation with AlbuMAX increased the expression of approximately 500-870 genes.

No MeSH data available.


Related in: MedlinePlus

Determination of knob density on the surface of IEs infected by 3D7 and FCR3 cultivated in the presence of AlbuMAX or human serum.The number of knobs was calculated from electron microscopy images of isolates cultivated under different culture conditions after enrichment for knob carrying IEs. **p < 0.01.
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f3: Determination of knob density on the surface of IEs infected by 3D7 and FCR3 cultivated in the presence of AlbuMAX or human serum.The number of knobs was calculated from electron microscopy images of isolates cultivated under different culture conditions after enrichment for knob carrying IEs. **p < 0.01.

Mentions: We found it interesting that cultivation of isolate 3D7 in the presence of AlbuMAX rather than human serum resulted in fewer knobs on the surface of IEs. Fewer knobs were also observed on FCR3, but the difference between AlbuMAX- and human serum-containing media was not significant (Fig. 3).


Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum.

Tilly AK, Thiede J, Metwally N, Lubiana P, Bachmann A, Roeder T, Rockliffe N, Lorenzen S, Tannich E, Gutsmann T, Bruchhaus I - Sci Rep (2015)

Determination of knob density on the surface of IEs infected by 3D7 and FCR3 cultivated in the presence of AlbuMAX or human serum.The number of knobs was calculated from electron microscopy images of isolates cultivated under different culture conditions after enrichment for knob carrying IEs. **p < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4645185&req=5

f3: Determination of knob density on the surface of IEs infected by 3D7 and FCR3 cultivated in the presence of AlbuMAX or human serum.The number of knobs was calculated from electron microscopy images of isolates cultivated under different culture conditions after enrichment for knob carrying IEs. **p < 0.01.
Mentions: We found it interesting that cultivation of isolate 3D7 in the presence of AlbuMAX rather than human serum resulted in fewer knobs on the surface of IEs. Fewer knobs were also observed on FCR3, but the difference between AlbuMAX- and human serum-containing media was not significant (Fig. 3).

Bottom Line: In addition, there was a greater reduction in the cytoadhesion of IEs to various endothelial receptors in the presence of AlbuMAX than in the presence of human serum.Surprisingly, cytoadhesion did not correlate with the presence or absence of knobs.Greater numbers of the variant surface antigen families RIFIN, STEVOR, and PfMC-2TM were found at the IE membrane when cultivated in the presence of AlbuMAX.

View Article: PubMed Central - PubMed

Affiliation: Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany.

ABSTRACT
In vitro cultivation of Plasmodium falciparum is critical for studying the biology of this parasite. However, it is likely that different in vitro cultivation conditions influence various aspects of the parasite's life cycle. In the present study two P. falciparum isolates were cultivated using the two most common methods, in which AlbuMAX or human serum as additives are used, and the results were compared. The type of cultivation influenced the knob structure of P. falciparum-infected erythrocytes (IEs). IEs cultivated with AlbuMAX had fewer knobs than those cultivated with human serum. Furthermore, knob size varied between isolates and is also depended on the culture medium. In addition, there was a greater reduction in the cytoadhesion of IEs to various endothelial receptors in the presence of AlbuMAX than in the presence of human serum. Surprisingly, cytoadhesion did not correlate with the presence or absence of knobs. Greater numbers of the variant surface antigen families RIFIN, STEVOR, and PfMC-2TM were found at the IE membrane when cultivated in the presence of AlbuMAX. Moreover, the type of cultivation had a marked influence on the transcriptome profile. Compared with cultivation with human serum, cultivation with AlbuMAX increased the expression of approximately 500-870 genes.

No MeSH data available.


Related in: MedlinePlus