Limits...
Prevalence and molecular characterizations of enterovirus D68 among children with acute respiratory infection in China between 2012 and 2014.

Xiao Q, Ren L, Zheng S, Wang L, Xie X, Deng Y, Zhao Y, Zhao X, Luo Z, Fu Z, Huang A, Liu E - Sci Rep (2015)

Bottom Line: Of these, 11 children were diagnosed with acute asthma exacerbation.EV-D68 was the predominant pathogen that evoked asthma exacerbation in September and October 2014.In conclusion, our results found that a history of recurrent wheezing may be a risk factor for the detection of EV-D68 and viral-induced asthma exacerbation may be a clinical feature of EV-D68 infection.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China.

ABSTRACT
EV-D68 is associated with respiratory tract infections (RTIs). Since its first isolation, EV-D68 has been detected sporadically. However, the US and Canada have experienced outbreaks of EV-D68 infections between August and December 2014. This study aimed to investigate the molecular epidemiology and clinical characteristics of EV-D68 in Chongqing, Southwestern China. From January 2012 to November 2014, 1876 nasopharyngeal aspirate specimens (NPAs) were collected from hospitalized children with RTIs. Among the 1876 NPAs, EV-D68 was detected in 19 samples (1.0%, 19/1876). Of these, 13 samples were detected in September and October 2014 (9.8%, 13/132). Phylogenetic analysis showed that all 13 strains detected in the 2014 Chongqing had high homology with the main strains of the 2014 US outbreak. Among the children with EV-D68 infection, 13 (68%) had a history of recurrent wheezing. A total of 13 children had a discharge diagnosis of asthma. Of these, 11 children were diagnosed with acute asthma exacerbation. EV-D68 was the predominant pathogen that evoked asthma exacerbation in September and October 2014. In conclusion, our results found that a history of recurrent wheezing may be a risk factor for the detection of EV-D68 and viral-induced asthma exacerbation may be a clinical feature of EV-D68 infection.

No MeSH data available.


Related in: MedlinePlus

Nucleotide regions of 5′untranslated regions of EV-D68 showed the deletion blocks preceding VP4.The difference in deletions (position 704 and 728) between clade B and clade C are indicated by boxes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4644992&req=5

f4: Nucleotide regions of 5′untranslated regions of EV-D68 showed the deletion blocks preceding VP4.The difference in deletions (position 704 and 728) between clade B and clade C are indicated by boxes.

Mentions: Kaida et al. first reported that EV-D68 Osaka strains have two deletions at nt 681–704 and 717–727 in contrast to the Fermon strain in the 5′UTR12. These EV-D68 Osaka strains were grouped into clade C according to phylogenetic analysis (Fig. 2). In the current analysis, fifteen Chongqing strains and seven strains from the 2014 US outbreak that were grouped into clade B also had two blocks of deletions at nt 681–703 and 717–728 in contrast with the Fermon strain (Fig. 4), which differed from clade C at nt 704 and 728. Tokarz et al. found that clade A only had a deletion at nt 681–7044. In the current analysis, four Chongqing strains (CQ2874, CQ2929, CQ5508, and CQ5753) and one strain from the 2014 US outbreak (US/KY/14-18953) that were grouped in clade A had a deletion at nt 682–704, which contrasted with the Fermon strain by a nucleic acid substitution at position 681 (Fig. 4).


Prevalence and molecular characterizations of enterovirus D68 among children with acute respiratory infection in China between 2012 and 2014.

Xiao Q, Ren L, Zheng S, Wang L, Xie X, Deng Y, Zhao Y, Zhao X, Luo Z, Fu Z, Huang A, Liu E - Sci Rep (2015)

Nucleotide regions of 5′untranslated regions of EV-D68 showed the deletion blocks preceding VP4.The difference in deletions (position 704 and 728) between clade B and clade C are indicated by boxes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644992&req=5

f4: Nucleotide regions of 5′untranslated regions of EV-D68 showed the deletion blocks preceding VP4.The difference in deletions (position 704 and 728) between clade B and clade C are indicated by boxes.
Mentions: Kaida et al. first reported that EV-D68 Osaka strains have two deletions at nt 681–704 and 717–727 in contrast to the Fermon strain in the 5′UTR12. These EV-D68 Osaka strains were grouped into clade C according to phylogenetic analysis (Fig. 2). In the current analysis, fifteen Chongqing strains and seven strains from the 2014 US outbreak that were grouped into clade B also had two blocks of deletions at nt 681–703 and 717–728 in contrast with the Fermon strain (Fig. 4), which differed from clade C at nt 704 and 728. Tokarz et al. found that clade A only had a deletion at nt 681–7044. In the current analysis, four Chongqing strains (CQ2874, CQ2929, CQ5508, and CQ5753) and one strain from the 2014 US outbreak (US/KY/14-18953) that were grouped in clade A had a deletion at nt 682–704, which contrasted with the Fermon strain by a nucleic acid substitution at position 681 (Fig. 4).

Bottom Line: Of these, 11 children were diagnosed with acute asthma exacerbation.EV-D68 was the predominant pathogen that evoked asthma exacerbation in September and October 2014.In conclusion, our results found that a history of recurrent wheezing may be a risk factor for the detection of EV-D68 and viral-induced asthma exacerbation may be a clinical feature of EV-D68 infection.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China.

ABSTRACT
EV-D68 is associated with respiratory tract infections (RTIs). Since its first isolation, EV-D68 has been detected sporadically. However, the US and Canada have experienced outbreaks of EV-D68 infections between August and December 2014. This study aimed to investigate the molecular epidemiology and clinical characteristics of EV-D68 in Chongqing, Southwestern China. From January 2012 to November 2014, 1876 nasopharyngeal aspirate specimens (NPAs) were collected from hospitalized children with RTIs. Among the 1876 NPAs, EV-D68 was detected in 19 samples (1.0%, 19/1876). Of these, 13 samples were detected in September and October 2014 (9.8%, 13/132). Phylogenetic analysis showed that all 13 strains detected in the 2014 Chongqing had high homology with the main strains of the 2014 US outbreak. Among the children with EV-D68 infection, 13 (68%) had a history of recurrent wheezing. A total of 13 children had a discharge diagnosis of asthma. Of these, 11 children were diagnosed with acute asthma exacerbation. EV-D68 was the predominant pathogen that evoked asthma exacerbation in September and October 2014. In conclusion, our results found that a history of recurrent wheezing may be a risk factor for the detection of EV-D68 and viral-induced asthma exacerbation may be a clinical feature of EV-D68 infection.

No MeSH data available.


Related in: MedlinePlus