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Prevalence and molecular characterizations of enterovirus D68 among children with acute respiratory infection in China between 2012 and 2014.

Xiao Q, Ren L, Zheng S, Wang L, Xie X, Deng Y, Zhao Y, Zhao X, Luo Z, Fu Z, Huang A, Liu E - Sci Rep (2015)

Bottom Line: Of these, 11 children were diagnosed with acute asthma exacerbation.EV-D68 was the predominant pathogen that evoked asthma exacerbation in September and October 2014.In conclusion, our results found that a history of recurrent wheezing may be a risk factor for the detection of EV-D68 and viral-induced asthma exacerbation may be a clinical feature of EV-D68 infection.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China.

ABSTRACT
EV-D68 is associated with respiratory tract infections (RTIs). Since its first isolation, EV-D68 has been detected sporadically. However, the US and Canada have experienced outbreaks of EV-D68 infections between August and December 2014. This study aimed to investigate the molecular epidemiology and clinical characteristics of EV-D68 in Chongqing, Southwestern China. From January 2012 to November 2014, 1876 nasopharyngeal aspirate specimens (NPAs) were collected from hospitalized children with RTIs. Among the 1876 NPAs, EV-D68 was detected in 19 samples (1.0%, 19/1876). Of these, 13 samples were detected in September and October 2014 (9.8%, 13/132). Phylogenetic analysis showed that all 13 strains detected in the 2014 Chongqing had high homology with the main strains of the 2014 US outbreak. Among the children with EV-D68 infection, 13 (68%) had a history of recurrent wheezing. A total of 13 children had a discharge diagnosis of asthma. Of these, 11 children were diagnosed with acute asthma exacerbation. EV-D68 was the predominant pathogen that evoked asthma exacerbation in September and October 2014. In conclusion, our results found that a history of recurrent wheezing may be a risk factor for the detection of EV-D68 and viral-induced asthma exacerbation may be a clinical feature of EV-D68 infection.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of selected EV-D68 strains based on the nucleotide sequence of 5′UTR to 3D genome regions.The 7059-bp fragments, which correspond to nt 145-7203 of the EV-D68 prototype strain (GenBank accession no. AY426531), were used to construct the phylogenetic tree. ● Strains detected in this study ▲ Sequences from the US in 2014.
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f3: Phylogenetic tree of selected EV-D68 strains based on the nucleotide sequence of 5′UTR to 3D genome regions.The 7059-bp fragments, which correspond to nt 145-7203 of the EV-D68 prototype strain (GenBank accession no. AY426531), were used to construct the phylogenetic tree. ● Strains detected in this study ▲ Sequences from the US in 2014.

Mentions: Sequence analysis of VP1 revealed that the 19 strains in Chongqing could be grouped into two clades (clade A and clade B) that were the main epidemic genotypes in Chongqing from 2009 to 2012 (Fig. 2)11. Thirteen strains of 2014 Chongqing detected, seven strains from the 2014 US outbreak, and most strains from the 2014 Netherlands detected were grouped into clade B. However, only two Chongqing strains from 2012 and 2013 could be grouped into clade B. The remaining four Chongqing strains were grouped into clade A (two in 2012, and two in 2013). Unfortunately, only thirteen Chongqing strains were nearly full-length nucleotide sequences, including ten strains from 2014, two strains from 2013, and one strain from 2012. Sequence analysis of nearly full-length nucleotide sequences found that the 13 clinical isolates detected in the current study had nucleotide sequence similarities of 89.9–99.8% with each other and similarities of 87.8–88.2% with the Fermon strain. Strains of clade B demonstrated similarities ranging between 94.3–99.8% (nucleotides). A phylogenetic tree constructed with nearly full-length nucleotide sequences revealed that the division of EV-D68 was consistent with Fig. 2 (Fig. 3).


Prevalence and molecular characterizations of enterovirus D68 among children with acute respiratory infection in China between 2012 and 2014.

Xiao Q, Ren L, Zheng S, Wang L, Xie X, Deng Y, Zhao Y, Zhao X, Luo Z, Fu Z, Huang A, Liu E - Sci Rep (2015)

Phylogenetic tree of selected EV-D68 strains based on the nucleotide sequence of 5′UTR to 3D genome regions.The 7059-bp fragments, which correspond to nt 145-7203 of the EV-D68 prototype strain (GenBank accession no. AY426531), were used to construct the phylogenetic tree. ● Strains detected in this study ▲ Sequences from the US in 2014.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644992&req=5

f3: Phylogenetic tree of selected EV-D68 strains based on the nucleotide sequence of 5′UTR to 3D genome regions.The 7059-bp fragments, which correspond to nt 145-7203 of the EV-D68 prototype strain (GenBank accession no. AY426531), were used to construct the phylogenetic tree. ● Strains detected in this study ▲ Sequences from the US in 2014.
Mentions: Sequence analysis of VP1 revealed that the 19 strains in Chongqing could be grouped into two clades (clade A and clade B) that were the main epidemic genotypes in Chongqing from 2009 to 2012 (Fig. 2)11. Thirteen strains of 2014 Chongqing detected, seven strains from the 2014 US outbreak, and most strains from the 2014 Netherlands detected were grouped into clade B. However, only two Chongqing strains from 2012 and 2013 could be grouped into clade B. The remaining four Chongqing strains were grouped into clade A (two in 2012, and two in 2013). Unfortunately, only thirteen Chongqing strains were nearly full-length nucleotide sequences, including ten strains from 2014, two strains from 2013, and one strain from 2012. Sequence analysis of nearly full-length nucleotide sequences found that the 13 clinical isolates detected in the current study had nucleotide sequence similarities of 89.9–99.8% with each other and similarities of 87.8–88.2% with the Fermon strain. Strains of clade B demonstrated similarities ranging between 94.3–99.8% (nucleotides). A phylogenetic tree constructed with nearly full-length nucleotide sequences revealed that the division of EV-D68 was consistent with Fig. 2 (Fig. 3).

Bottom Line: Of these, 11 children were diagnosed with acute asthma exacerbation.EV-D68 was the predominant pathogen that evoked asthma exacerbation in September and October 2014.In conclusion, our results found that a history of recurrent wheezing may be a risk factor for the detection of EV-D68 and viral-induced asthma exacerbation may be a clinical feature of EV-D68 infection.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China.

ABSTRACT
EV-D68 is associated with respiratory tract infections (RTIs). Since its first isolation, EV-D68 has been detected sporadically. However, the US and Canada have experienced outbreaks of EV-D68 infections between August and December 2014. This study aimed to investigate the molecular epidemiology and clinical characteristics of EV-D68 in Chongqing, Southwestern China. From January 2012 to November 2014, 1876 nasopharyngeal aspirate specimens (NPAs) were collected from hospitalized children with RTIs. Among the 1876 NPAs, EV-D68 was detected in 19 samples (1.0%, 19/1876). Of these, 13 samples were detected in September and October 2014 (9.8%, 13/132). Phylogenetic analysis showed that all 13 strains detected in the 2014 Chongqing had high homology with the main strains of the 2014 US outbreak. Among the children with EV-D68 infection, 13 (68%) had a history of recurrent wheezing. A total of 13 children had a discharge diagnosis of asthma. Of these, 11 children were diagnosed with acute asthma exacerbation. EV-D68 was the predominant pathogen that evoked asthma exacerbation in September and October 2014. In conclusion, our results found that a history of recurrent wheezing may be a risk factor for the detection of EV-D68 and viral-induced asthma exacerbation may be a clinical feature of EV-D68 infection.

No MeSH data available.


Related in: MedlinePlus