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Phylogenetic Analysis of Hemagglutinin Genes of H9N2 Avian Influenza Viruses Isolated from Chickens in Shandong, China, between 1998 and 2013.

Zhao Y, Li S, Zhou Y, Song W, Tang Y, Pang Q, Miao Z - Biomed Res Int (2015)

Bottom Line: The substitution showed that H9N2 AIVs had the potential affinity to bind to human-like receptor.The currently prevalent H9N2 AIVs in Shandong belonged to the lineage h9.4.2.5 which are different from the vaccine strain SS/94 clade h9.4.2.3.Therefore, the long-term surveillance of H9N2 AIVs is of significance to combat the possible H9N2 AIV outbreaks.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Taishan Medical University, Tai'an 271000, China.

ABSTRACT
Since H9N2 avian influenza virus (AIV) was first isolated in Guangdong province of China, the virus has been circulating in chicken flocks in mainland China. However, a systematic phylogenetic analysis of H9N2 AIV from chickens in Shandong of China has not been conducted. Based on hemagglutinin (HA) gene sequences of H9N2 AIVs isolated from chickens in Shandong of China between 1998 and 2013, genetic evolution of 35 HA gene sequences was systematically analyzed in this study. Our findings showed that the majority of H9N2 AIVs (21 out of 35) belonged to the lineage h9.4.2.5. Most of isolates (33 out of 35) had a PSRSSR↓GLF motif in HA cleavage site. Importantly, 29 out of these 35 isolates had an amino acid exchange (Q226L) in the receptor-binding site. The substitution showed that H9N2 AIVs had the potential affinity to bind to human-like receptor. The currently prevalent H9N2 AIVs in Shandong belonged to the lineage h9.4.2.5 which are different from the vaccine strain SS/94 clade h9.4.2.3. Therefore, the long-term surveillance of H9N2 AIVs is of significance to combat the possible H9N2 AIV outbreaks.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of HA genes of H9N2 AIVs used in this study. The black squares indicate the representative strains of distinct H9 lineages. The black triangles indicate vaccine strains.
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fig1: Phylogenetic tree of HA genes of H9N2 AIVs used in this study. The black squares indicate the representative strains of distinct H9 lineages. The black triangles indicate vaccine strains.

Mentions: All of 35 H9N2 AIVs belonged to the lineage h9.4.2 represented by Y280 or BJ194. The lineage h9.4.2 is divided into 6 tertiary levels: h9.4.2.1, h9.4.2.2, h9.4.2.3, h9.4.2.4, h9.4.2.5, and h9.4.2.6 [23]. The majority of H9N2 AIVs (21 out of 35) belonged to the lineage h9.4.2.5 represented by strains Ck/ZJ/HE6/09 and Ck/GX/55/05 [24]. All of 12 H9N2 AIVs isolated between 2010 and 2013 belonged to the lineage h9.4.2.5. Vaccine strains SS/94 and 6/96 belonged to the linage h9.4.2.3, and vaccine strain F/98 was clustered in the lineage h9.4.2.1 (Figure 1).


Phylogenetic Analysis of Hemagglutinin Genes of H9N2 Avian Influenza Viruses Isolated from Chickens in Shandong, China, between 1998 and 2013.

Zhao Y, Li S, Zhou Y, Song W, Tang Y, Pang Q, Miao Z - Biomed Res Int (2015)

Phylogenetic tree of HA genes of H9N2 AIVs used in this study. The black squares indicate the representative strains of distinct H9 lineages. The black triangles indicate vaccine strains.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644933&req=5

fig1: Phylogenetic tree of HA genes of H9N2 AIVs used in this study. The black squares indicate the representative strains of distinct H9 lineages. The black triangles indicate vaccine strains.
Mentions: All of 35 H9N2 AIVs belonged to the lineage h9.4.2 represented by Y280 or BJ194. The lineage h9.4.2 is divided into 6 tertiary levels: h9.4.2.1, h9.4.2.2, h9.4.2.3, h9.4.2.4, h9.4.2.5, and h9.4.2.6 [23]. The majority of H9N2 AIVs (21 out of 35) belonged to the lineage h9.4.2.5 represented by strains Ck/ZJ/HE6/09 and Ck/GX/55/05 [24]. All of 12 H9N2 AIVs isolated between 2010 and 2013 belonged to the lineage h9.4.2.5. Vaccine strains SS/94 and 6/96 belonged to the linage h9.4.2.3, and vaccine strain F/98 was clustered in the lineage h9.4.2.1 (Figure 1).

Bottom Line: The substitution showed that H9N2 AIVs had the potential affinity to bind to human-like receptor.The currently prevalent H9N2 AIVs in Shandong belonged to the lineage h9.4.2.5 which are different from the vaccine strain SS/94 clade h9.4.2.3.Therefore, the long-term surveillance of H9N2 AIVs is of significance to combat the possible H9N2 AIV outbreaks.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Taishan Medical University, Tai'an 271000, China.

ABSTRACT
Since H9N2 avian influenza virus (AIV) was first isolated in Guangdong province of China, the virus has been circulating in chicken flocks in mainland China. However, a systematic phylogenetic analysis of H9N2 AIV from chickens in Shandong of China has not been conducted. Based on hemagglutinin (HA) gene sequences of H9N2 AIVs isolated from chickens in Shandong of China between 1998 and 2013, genetic evolution of 35 HA gene sequences was systematically analyzed in this study. Our findings showed that the majority of H9N2 AIVs (21 out of 35) belonged to the lineage h9.4.2.5. Most of isolates (33 out of 35) had a PSRSSR↓GLF motif in HA cleavage site. Importantly, 29 out of these 35 isolates had an amino acid exchange (Q226L) in the receptor-binding site. The substitution showed that H9N2 AIVs had the potential affinity to bind to human-like receptor. The currently prevalent H9N2 AIVs in Shandong belonged to the lineage h9.4.2.5 which are different from the vaccine strain SS/94 clade h9.4.2.3. Therefore, the long-term surveillance of H9N2 AIVs is of significance to combat the possible H9N2 AIV outbreaks.

No MeSH data available.


Related in: MedlinePlus