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Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB Pathway.

Tan WS, Arulselvan P, Karthivashan G, Fakurazi S - Mediators Inflamm. (2015)

Bottom Line: However, it significantly increased the production of IL-10 and IκB-α (inhibitor of κB) in a concentration dependent manner (100 μg/mL and 200 μg/mL).Conclusion.These results suggest that 80% hydroethanolic extract of M. oleifera flower has anti-inflammatory action related to its inhibition of NO, PGE2, proinflammatory cytokines, and inflammatory mediator's production in LPS-stimulated macrophages through preventing degradation of IκB-α in NF-κB signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

ABSTRACT
Aim of Study. Moringa oleifera Lam. (M. oleifera) possess highest concentration of antioxidant bioactive compounds and is anticipated to be used as an alternative medicine for inflammation. In the present study, we investigated the anti-inflammatory activity of 80% hydroethanolic extract of M. oleifera flower on proinflammatory mediators and cytokines produced in lipopolysaccharide- (LPS-) induced RAW 264.7 macrophages. Materials and Methods. Cell cytotoxicity was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide (NO) production was quantified through Griess reaction while proinflammatory cytokines and other key inflammatory markers were assessed through enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Results. Hydroethanolic extract of M. oleifera flower significantly suppressed the secretion and expression of NO, prostaglandin E2 (PGE2), interleukin- (IL-) 6, IL-1β, tumor necrosis factor-alpha (TNF-α), nuclear factor-kappa B (NF-κB), inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2). However, it significantly increased the production of IL-10 and IκB-α (inhibitor of κB) in a concentration dependent manner (100 μg/mL and 200 μg/mL). Conclusion. These results suggest that 80% hydroethanolic extract of M. oleifera flower has anti-inflammatory action related to its inhibition of NO, PGE2, proinflammatory cytokines, and inflammatory mediator's production in LPS-stimulated macrophages through preventing degradation of IκB-α in NF-κB signaling pathway.

No MeSH data available.


Related in: MedlinePlus

Effects of 80% hydroethanolic M. oleifera bioactive flower extract on the viability of RAW 264.7 macrophages. A density of 1 × 105 cells/well of macrophages were seeded in 96-well plate and incubated with various concentrations of flower extract for 24 h. Cell viability was determined by MTT assay. The data are presented as mean ± SD of three independent experiments. ∗∗∗p < 0.001, ∗∗p < 0.01 versus culture media without flower extract which act as control.
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fig2: Effects of 80% hydroethanolic M. oleifera bioactive flower extract on the viability of RAW 264.7 macrophages. A density of 1 × 105 cells/well of macrophages were seeded in 96-well plate and incubated with various concentrations of flower extract for 24 h. Cell viability was determined by MTT assay. The data are presented as mean ± SD of three independent experiments. ∗∗∗p < 0.001, ∗∗p < 0.01 versus culture media without flower extract which act as control.

Mentions: MTT reduction assay was used to access the cytotoxicity effect of 80% hydroethanolic M. oleifera flower extract at concentration ranging from the lowest to highest (15.625–1000 μg/mL) on RAW 264.7 macrophages. The cytotoxicity potential of flower extract on macrophages was presented in Figure 2. The results showed that increasing concentrations of hydroethanolic M. oleifera flower extract have caused reduction of cell viability. However, hydroethanolic M. oleifera flower extract did not exhibit any toxicity to macrophages at concentrations ranging from 15.625 to 125 µg/mL. According to the cytotoxicity investigations, the concentrations at 100 μg/mL and 200 μg/mL were chosen for further anti-inflammatory experiments.


Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB Pathway.

Tan WS, Arulselvan P, Karthivashan G, Fakurazi S - Mediators Inflamm. (2015)

Effects of 80% hydroethanolic M. oleifera bioactive flower extract on the viability of RAW 264.7 macrophages. A density of 1 × 105 cells/well of macrophages were seeded in 96-well plate and incubated with various concentrations of flower extract for 24 h. Cell viability was determined by MTT assay. The data are presented as mean ± SD of three independent experiments. ∗∗∗p < 0.001, ∗∗p < 0.01 versus culture media without flower extract which act as control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644847&req=5

fig2: Effects of 80% hydroethanolic M. oleifera bioactive flower extract on the viability of RAW 264.7 macrophages. A density of 1 × 105 cells/well of macrophages were seeded in 96-well plate and incubated with various concentrations of flower extract for 24 h. Cell viability was determined by MTT assay. The data are presented as mean ± SD of three independent experiments. ∗∗∗p < 0.001, ∗∗p < 0.01 versus culture media without flower extract which act as control.
Mentions: MTT reduction assay was used to access the cytotoxicity effect of 80% hydroethanolic M. oleifera flower extract at concentration ranging from the lowest to highest (15.625–1000 μg/mL) on RAW 264.7 macrophages. The cytotoxicity potential of flower extract on macrophages was presented in Figure 2. The results showed that increasing concentrations of hydroethanolic M. oleifera flower extract have caused reduction of cell viability. However, hydroethanolic M. oleifera flower extract did not exhibit any toxicity to macrophages at concentrations ranging from 15.625 to 125 µg/mL. According to the cytotoxicity investigations, the concentrations at 100 μg/mL and 200 μg/mL were chosen for further anti-inflammatory experiments.

Bottom Line: However, it significantly increased the production of IL-10 and IκB-α (inhibitor of κB) in a concentration dependent manner (100 μg/mL and 200 μg/mL).Conclusion.These results suggest that 80% hydroethanolic extract of M. oleifera flower has anti-inflammatory action related to its inhibition of NO, PGE2, proinflammatory cytokines, and inflammatory mediator's production in LPS-stimulated macrophages through preventing degradation of IκB-α in NF-κB signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

ABSTRACT
Aim of Study. Moringa oleifera Lam. (M. oleifera) possess highest concentration of antioxidant bioactive compounds and is anticipated to be used as an alternative medicine for inflammation. In the present study, we investigated the anti-inflammatory activity of 80% hydroethanolic extract of M. oleifera flower on proinflammatory mediators and cytokines produced in lipopolysaccharide- (LPS-) induced RAW 264.7 macrophages. Materials and Methods. Cell cytotoxicity was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide (NO) production was quantified through Griess reaction while proinflammatory cytokines and other key inflammatory markers were assessed through enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Results. Hydroethanolic extract of M. oleifera flower significantly suppressed the secretion and expression of NO, prostaglandin E2 (PGE2), interleukin- (IL-) 6, IL-1β, tumor necrosis factor-alpha (TNF-α), nuclear factor-kappa B (NF-κB), inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2). However, it significantly increased the production of IL-10 and IκB-α (inhibitor of κB) in a concentration dependent manner (100 μg/mL and 200 μg/mL). Conclusion. These results suggest that 80% hydroethanolic extract of M. oleifera flower has anti-inflammatory action related to its inhibition of NO, PGE2, proinflammatory cytokines, and inflammatory mediator's production in LPS-stimulated macrophages through preventing degradation of IκB-α in NF-κB signaling pathway.

No MeSH data available.


Related in: MedlinePlus