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Effects of Lutein and Zeaxanthin on LPS-Induced Secretion of IL-8 by Uveal Melanocytes and Relevant Signal Pathways.

Chao SC, Vagaggini T, Nien CW, Huang SC, Lin HY - J Ophthalmol (2015)

Bottom Line: Lutein and zeaxanthin did not affect the constitutive secretion of IL-8.Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels.The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan ; Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan ; Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beitun District, Taichung 40601, Taiwan.

ABSTRACT
The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01-1 μg/mL) and lutein and zeaxanthin (1-10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner. LPS significantly increased NF-κB levels in cell nuclear extracts and p-JNK levels in the cell lysates from UM, but not p-p38 MAPK and p-ERG. Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels. The present study demonstrated that lutein and zeaxanthin inhibited LPS-induced secretion of IL-8 in cultured UM via JNK and NF-κB signal pathways. The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

No MeSH data available.


Related in: MedlinePlus

LPS, lutein, and zeaxanthin and various signal pathways. UM were seeded into the culture dishes, treated with LPS with or without previous treatment of lutein or zeaxanthin. p-MAPKs in the cells and NF-κB levels in the cell nuclear extracts were determined by ELISA kit and expressed as percentage of the controls. LPS significantly increased p-JNK (a) and NF-κB levels (d) (p < 0.05 as compared with cells not treated with LPS) but not p-p38 MAPK (c) and p-ERG1/2 levels (d) in cultured UM.
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fig5: LPS, lutein, and zeaxanthin and various signal pathways. UM were seeded into the culture dishes, treated with LPS with or without previous treatment of lutein or zeaxanthin. p-MAPKs in the cells and NF-κB levels in the cell nuclear extracts were determined by ELISA kit and expressed as percentage of the controls. LPS significantly increased p-JNK (a) and NF-κB levels (d) (p < 0.05 as compared with cells not treated with LPS) but not p-p38 MAPK (c) and p-ERG1/2 levels (d) in cultured UM.

Mentions: LPS at 0.1 μg/mL level significantly increased p-JNK levels (p < 0.05 as compared with cells not treated with LPS; Figure 5(a)) but not p-p38 MAPK and p-ERG1/2 levels in cultured UM (p > 0.05, Figures 5(b) and 5(c)). Addition of lutein or zeaxanthin significantly reduced LPS-induced increase of p-JNK levels (both p < 0.05, Figure 5(a)) but did not affect p-p38 MAPK and p-ERG1/2 levels (both p > 0.05, Figures 5(b) and 5(c)) in cultured UM. NF-κB levels in cell nuclear extracts from cells treated with LPS were significantly greater than those from cells not treated with LPS (p < 0.05, Figure 5(d)). Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB levels in cell nuclear extracts (both p < 0.05, Figure 5(d)). These results suggested that JNK1/2 and NF-κB (but not p38 MAPK and ERK1/2) play an important role in LPS-induced increased secretion of IL-8 and in the inhibitory effects of lutein and zeaxanthin on LPS-induced increased secretion of IL-8.


Effects of Lutein and Zeaxanthin on LPS-Induced Secretion of IL-8 by Uveal Melanocytes and Relevant Signal Pathways.

Chao SC, Vagaggini T, Nien CW, Huang SC, Lin HY - J Ophthalmol (2015)

LPS, lutein, and zeaxanthin and various signal pathways. UM were seeded into the culture dishes, treated with LPS with or without previous treatment of lutein or zeaxanthin. p-MAPKs in the cells and NF-κB levels in the cell nuclear extracts were determined by ELISA kit and expressed as percentage of the controls. LPS significantly increased p-JNK (a) and NF-κB levels (d) (p < 0.05 as compared with cells not treated with LPS) but not p-p38 MAPK (c) and p-ERG1/2 levels (d) in cultured UM.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig5: LPS, lutein, and zeaxanthin and various signal pathways. UM were seeded into the culture dishes, treated with LPS with or without previous treatment of lutein or zeaxanthin. p-MAPKs in the cells and NF-κB levels in the cell nuclear extracts were determined by ELISA kit and expressed as percentage of the controls. LPS significantly increased p-JNK (a) and NF-κB levels (d) (p < 0.05 as compared with cells not treated with LPS) but not p-p38 MAPK (c) and p-ERG1/2 levels (d) in cultured UM.
Mentions: LPS at 0.1 μg/mL level significantly increased p-JNK levels (p < 0.05 as compared with cells not treated with LPS; Figure 5(a)) but not p-p38 MAPK and p-ERG1/2 levels in cultured UM (p > 0.05, Figures 5(b) and 5(c)). Addition of lutein or zeaxanthin significantly reduced LPS-induced increase of p-JNK levels (both p < 0.05, Figure 5(a)) but did not affect p-p38 MAPK and p-ERG1/2 levels (both p > 0.05, Figures 5(b) and 5(c)) in cultured UM. NF-κB levels in cell nuclear extracts from cells treated with LPS were significantly greater than those from cells not treated with LPS (p < 0.05, Figure 5(d)). Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB levels in cell nuclear extracts (both p < 0.05, Figure 5(d)). These results suggested that JNK1/2 and NF-κB (but not p38 MAPK and ERK1/2) play an important role in LPS-induced increased secretion of IL-8 and in the inhibitory effects of lutein and zeaxanthin on LPS-induced increased secretion of IL-8.

Bottom Line: Lutein and zeaxanthin did not affect the constitutive secretion of IL-8.Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels.The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan ; Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan ; Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beitun District, Taichung 40601, Taiwan.

ABSTRACT
The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01-1 μg/mL) and lutein and zeaxanthin (1-10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner. LPS significantly increased NF-κB levels in cell nuclear extracts and p-JNK levels in the cell lysates from UM, but not p-p38 MAPK and p-ERG. Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels. The present study demonstrated that lutein and zeaxanthin inhibited LPS-induced secretion of IL-8 in cultured UM via JNK and NF-κB signal pathways. The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

No MeSH data available.


Related in: MedlinePlus