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Effects of Lutein and Zeaxanthin on LPS-Induced Secretion of IL-8 by Uveal Melanocytes and Relevant Signal Pathways.

Chao SC, Vagaggini T, Nien CW, Huang SC, Lin HY - J Ophthalmol (2015)

Bottom Line: Lutein and zeaxanthin did not affect the constitutive secretion of IL-8.Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels.The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan ; Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan ; Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beitun District, Taichung 40601, Taiwan.

ABSTRACT
The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01-1 μg/mL) and lutein and zeaxanthin (1-10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner. LPS significantly increased NF-κB levels in cell nuclear extracts and p-JNK levels in the cell lysates from UM, but not p-p38 MAPK and p-ERG. Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels. The present study demonstrated that lutein and zeaxanthin inhibited LPS-induced secretion of IL-8 in cultured UM via JNK and NF-κB signal pathways. The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

No MeSH data available.


Related in: MedlinePlus

Lutein and zeaxanthinon constitutive secretion of IL-8 by uveal melanocytes. Cells were plated into 24-well plates and treated with lutein or zeaxanthin at different levels. IL-8 protein levels (expressed as percentage of the controls) in cell supernatants from cells treated with lutein (a) or zeaxanthin (b) at different levels did not significantly differ from those from cells not treated with lutein and zeaxanthin (p > 0.05).
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fig3: Lutein and zeaxanthinon constitutive secretion of IL-8 by uveal melanocytes. Cells were plated into 24-well plates and treated with lutein or zeaxanthin at different levels. IL-8 protein levels (expressed as percentage of the controls) in cell supernatants from cells treated with lutein (a) or zeaxanthin (b) at different levels did not significantly differ from those from cells not treated with lutein and zeaxanthin (p > 0.05).

Mentions: IL-8 protein levels in cell supernatants from cells treated with lutein or zeaxanthin (1, 3, and 10 μM) did not significantly differ from those from cells not treated with lutein and zeaxanthin (p > 0.05, Figure 3), suggesting that lutein and zeaxanthin do not affect the constitutive secretion of IL-8 from UM.


Effects of Lutein and Zeaxanthin on LPS-Induced Secretion of IL-8 by Uveal Melanocytes and Relevant Signal Pathways.

Chao SC, Vagaggini T, Nien CW, Huang SC, Lin HY - J Ophthalmol (2015)

Lutein and zeaxanthinon constitutive secretion of IL-8 by uveal melanocytes. Cells were plated into 24-well plates and treated with lutein or zeaxanthin at different levels. IL-8 protein levels (expressed as percentage of the controls) in cell supernatants from cells treated with lutein (a) or zeaxanthin (b) at different levels did not significantly differ from those from cells not treated with lutein and zeaxanthin (p > 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644841&req=5

fig3: Lutein and zeaxanthinon constitutive secretion of IL-8 by uveal melanocytes. Cells were plated into 24-well plates and treated with lutein or zeaxanthin at different levels. IL-8 protein levels (expressed as percentage of the controls) in cell supernatants from cells treated with lutein (a) or zeaxanthin (b) at different levels did not significantly differ from those from cells not treated with lutein and zeaxanthin (p > 0.05).
Mentions: IL-8 protein levels in cell supernatants from cells treated with lutein or zeaxanthin (1, 3, and 10 μM) did not significantly differ from those from cells not treated with lutein and zeaxanthin (p > 0.05, Figure 3), suggesting that lutein and zeaxanthin do not affect the constitutive secretion of IL-8 from UM.

Bottom Line: Lutein and zeaxanthin did not affect the constitutive secretion of IL-8.Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels.The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan ; Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan ; Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beitun District, Taichung 40601, Taiwan.

ABSTRACT
The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01-1 μg/mL) and lutein and zeaxanthin (1-10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner. LPS significantly increased NF-κB levels in cell nuclear extracts and p-JNK levels in the cell lysates from UM, but not p-p38 MAPK and p-ERG. Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels. The present study demonstrated that lutein and zeaxanthin inhibited LPS-induced secretion of IL-8 in cultured UM via JNK and NF-κB signal pathways. The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

No MeSH data available.


Related in: MedlinePlus