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Effects of Lutein and Zeaxanthin on LPS-Induced Secretion of IL-8 by Uveal Melanocytes and Relevant Signal Pathways.

Chao SC, Vagaggini T, Nien CW, Huang SC, Lin HY - J Ophthalmol (2015)

Bottom Line: Lutein and zeaxanthin did not affect the constitutive secretion of IL-8.Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels.The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan ; Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan ; Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beitun District, Taichung 40601, Taiwan.

ABSTRACT
The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01-1 μg/mL) and lutein and zeaxanthin (1-10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner. LPS significantly increased NF-κB levels in cell nuclear extracts and p-JNK levels in the cell lysates from UM, but not p-p38 MAPK and p-ERG. Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels. The present study demonstrated that lutein and zeaxanthin inhibited LPS-induced secretion of IL-8 in cultured UM via JNK and NF-κB signal pathways. The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

No MeSH data available.


Related in: MedlinePlus

Effects of LPS, lutein, and zeaxanthin on viability of uveal melanocytes (UM). Cells were seeded into 96-well plates and treated with LPS, lutein, and zeaxanthin at different levels. Cell viability was determined by MTT assay (see Material and Methods). LPS (a), lutein (b), and zeaxanthin (c) at all tested levels did not affect the viability of UM (expressed as percentage of the controls) (p > 0.05).
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fig1: Effects of LPS, lutein, and zeaxanthin on viability of uveal melanocytes (UM). Cells were seeded into 96-well plates and treated with LPS, lutein, and zeaxanthin at different levels. Cell viability was determined by MTT assay (see Material and Methods). LPS (a), lutein (b), and zeaxanthin (c) at all tested levels did not affect the viability of UM (expressed as percentage of the controls) (p > 0.05).

Mentions: MTT assay showed that LPS at the final levels of 0.01, 0.1, and 1 μg/mL did not influence the cell viability of cultured human UM (p > 0.05, compared with cells not treated with LPS) (Figure 1(a)). Lutein and zeaxanthin at the final levels of 1, 3, and 10 μM also had no effects on the cell viability of cultured UM (p > 0.05, compared with cells not treated with LPS) (Figures 1(b) and 1(c)). Therefore, level ranges of 0.01–1 μg/mL of LPS and 1–10 μM of lutein and zeaxanthin were chosen for subsequent experiments.


Effects of Lutein and Zeaxanthin on LPS-Induced Secretion of IL-8 by Uveal Melanocytes and Relevant Signal Pathways.

Chao SC, Vagaggini T, Nien CW, Huang SC, Lin HY - J Ophthalmol (2015)

Effects of LPS, lutein, and zeaxanthin on viability of uveal melanocytes (UM). Cells were seeded into 96-well plates and treated with LPS, lutein, and zeaxanthin at different levels. Cell viability was determined by MTT assay (see Material and Methods). LPS (a), lutein (b), and zeaxanthin (c) at all tested levels did not affect the viability of UM (expressed as percentage of the controls) (p > 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644841&req=5

fig1: Effects of LPS, lutein, and zeaxanthin on viability of uveal melanocytes (UM). Cells were seeded into 96-well plates and treated with LPS, lutein, and zeaxanthin at different levels. Cell viability was determined by MTT assay (see Material and Methods). LPS (a), lutein (b), and zeaxanthin (c) at all tested levels did not affect the viability of UM (expressed as percentage of the controls) (p > 0.05).
Mentions: MTT assay showed that LPS at the final levels of 0.01, 0.1, and 1 μg/mL did not influence the cell viability of cultured human UM (p > 0.05, compared with cells not treated with LPS) (Figure 1(a)). Lutein and zeaxanthin at the final levels of 1, 3, and 10 μM also had no effects on the cell viability of cultured UM (p > 0.05, compared with cells not treated with LPS) (Figures 1(b) and 1(c)). Therefore, level ranges of 0.01–1 μg/mL of LPS and 1–10 μM of lutein and zeaxanthin were chosen for subsequent experiments.

Bottom Line: Lutein and zeaxanthin did not affect the constitutive secretion of IL-8.Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels.The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan ; Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan ; Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beitun District, Taichung 40601, Taiwan.

ABSTRACT
The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01-1 μg/mL) and lutein and zeaxanthin (1-10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner. LPS significantly increased NF-κB levels in cell nuclear extracts and p-JNK levels in the cell lysates from UM, but not p-p38 MAPK and p-ERG. Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels. The present study demonstrated that lutein and zeaxanthin inhibited LPS-induced secretion of IL-8 in cultured UM via JNK and NF-κB signal pathways. The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.

No MeSH data available.


Related in: MedlinePlus