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Involutin is an Fe3+ reductant secreted by the ectomycorrhizal fungus Paxillus involutus during Fenton-based decomposition of organic matter.

Shah F, Schwenk D, Nicolás C, Persson P, Hoffmeister D, Tunlid A - Appl. Environ. Microbiol. (2015)

Bottom Line: Activity-guided purification was performed to isolate the Fe(3+)-reducing principle secreted by P. involutus during growth on a maize compost extract.The Fe(3+)-reducing activity correlated with the presence of one compound.We also demonstrated that in the presence of H2O2, involutin has the capacity to drive an in vitro Fenton reaction via Fe(3+) reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Microbial Ecology Group, Lund University, Lund, Sweden Firoz.Shah@biol.lu.se.

No MeSH data available.


Related in: MedlinePlus

In vitro Fenton reaction with involutin. Shown is the generation of h-TPA, an indicator of hydroxyl radical production, by involutin and 2,6-DMHQ, as measured by fluorescence spectroscopy at an excitation wavelength of 315 nm and an emission wavelength of 425 nm. Error bars indicate standard errors (n = 3).
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Figure 4: In vitro Fenton reaction with involutin. Shown is the generation of h-TPA, an indicator of hydroxyl radical production, by involutin and 2,6-DMHQ, as measured by fluorescence spectroscopy at an excitation wavelength of 315 nm and an emission wavelength of 425 nm. Error bars indicate standard errors (n = 3).

Mentions: To examine whether involutin may play a role in the production of hydroxyl radicals during organic matter decomposition by P. involutus, we analyzed the ability of purified involutin to drive Fenton chemistry in vitro via Fe3+ reduction. In the presence of involutin, FeCl3, and H2O2, significant amounts of h-TPA were produced, suggesting the production of hydroxyl radicals (Fig. 4). Under these conditions, the generation of h-TPA by involutin was similar to that of the hydroquinone 2,6-DMHQ. In the absence of H2O2, no hydroxyl radicals were formed in the presence of either involutin or 2,6-DMHQ. It has been shown previously that hydroxyl radical production requires the reduction of Fe3+ in the presence of H2O2 (33). Thus, in our experimental system, the metabolite involutin drives a Fenton reaction via Fe3+ reduction.


Involutin is an Fe3+ reductant secreted by the ectomycorrhizal fungus Paxillus involutus during Fenton-based decomposition of organic matter.

Shah F, Schwenk D, Nicolás C, Persson P, Hoffmeister D, Tunlid A - Appl. Environ. Microbiol. (2015)

In vitro Fenton reaction with involutin. Shown is the generation of h-TPA, an indicator of hydroxyl radical production, by involutin and 2,6-DMHQ, as measured by fluorescence spectroscopy at an excitation wavelength of 315 nm and an emission wavelength of 425 nm. Error bars indicate standard errors (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644656&req=5

Figure 4: In vitro Fenton reaction with involutin. Shown is the generation of h-TPA, an indicator of hydroxyl radical production, by involutin and 2,6-DMHQ, as measured by fluorescence spectroscopy at an excitation wavelength of 315 nm and an emission wavelength of 425 nm. Error bars indicate standard errors (n = 3).
Mentions: To examine whether involutin may play a role in the production of hydroxyl radicals during organic matter decomposition by P. involutus, we analyzed the ability of purified involutin to drive Fenton chemistry in vitro via Fe3+ reduction. In the presence of involutin, FeCl3, and H2O2, significant amounts of h-TPA were produced, suggesting the production of hydroxyl radicals (Fig. 4). Under these conditions, the generation of h-TPA by involutin was similar to that of the hydroquinone 2,6-DMHQ. In the absence of H2O2, no hydroxyl radicals were formed in the presence of either involutin or 2,6-DMHQ. It has been shown previously that hydroxyl radical production requires the reduction of Fe3+ in the presence of H2O2 (33). Thus, in our experimental system, the metabolite involutin drives a Fenton reaction via Fe3+ reduction.

Bottom Line: Activity-guided purification was performed to isolate the Fe(3+)-reducing principle secreted by P. involutus during growth on a maize compost extract.The Fe(3+)-reducing activity correlated with the presence of one compound.We also demonstrated that in the presence of H2O2, involutin has the capacity to drive an in vitro Fenton reaction via Fe(3+) reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Microbial Ecology Group, Lund University, Lund, Sweden Firoz.Shah@biol.lu.se.

No MeSH data available.


Related in: MedlinePlus