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Involutin is an Fe3+ reductant secreted by the ectomycorrhizal fungus Paxillus involutus during Fenton-based decomposition of organic matter.

Shah F, Schwenk D, Nicolás C, Persson P, Hoffmeister D, Tunlid A - Appl. Environ. Microbiol. (2015)

Bottom Line: Activity-guided purification was performed to isolate the Fe(3+)-reducing principle secreted by P. involutus during growth on a maize compost extract.The Fe(3+)-reducing activity correlated with the presence of one compound.We also demonstrated that in the presence of H2O2, involutin has the capacity to drive an in vitro Fenton reaction via Fe(3+) reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Microbial Ecology Group, Lund University, Lund, Sweden Firoz.Shah@biol.lu.se.

No MeSH data available.


Related in: MedlinePlus

Analysis of secondary metabolites secreted by P. involutus during growth on organic matter. Shown are total ion current profiles from the HRESIMS (negative mode) spectra of the MH0 SPEtOAc eluate, recovered from the initial organic material (A), and of the MH7 SPEtOAc (B) and MH7 SPMeOH (C) fractions, sampled after 7 days of growth. Note that the MH7 SPEtOAc sample contains one major peak, indicated by the arrow (involutin), that is not present in the MH0 sample. Involutin is also present in MH7 SPMeOH. (Inset) HRESIMS profile of involutin.
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Figure 2: Analysis of secondary metabolites secreted by P. involutus during growth on organic matter. Shown are total ion current profiles from the HRESIMS (negative mode) spectra of the MH0 SPEtOAc eluate, recovered from the initial organic material (A), and of the MH7 SPEtOAc (B) and MH7 SPMeOH (C) fractions, sampled after 7 days of growth. Note that the MH7 SPEtOAc sample contains one major peak, indicated by the arrow (involutin), that is not present in the MH0 sample. Involutin is also present in MH7 SPMeOH. (Inset) HRESIMS profile of involutin.

Mentions: LC-MS analysis of the MH0 SPEtOAc, MH7 SPEtOAc, and MH7 SPMeOH fractions revealed that one major compound was formed during the decomposition of the organic material (Fig. 2). Based on the HPLC and HRESIMS total-ion chromatogram, the signal corresponded to a single compound with m/z 313.0719 and thus was tentatively identified as involutin (Fig. 2B, inset). The total ion current profiles for the MH7 SPEtOAc and MH7 SPMeOH fractions were similar except that the peak associated with involutin was considerably lower in the MH7 SPMeOH fraction than in the MH7 SPEtOAc fraction (Fig. 2B and C).


Involutin is an Fe3+ reductant secreted by the ectomycorrhizal fungus Paxillus involutus during Fenton-based decomposition of organic matter.

Shah F, Schwenk D, Nicolás C, Persson P, Hoffmeister D, Tunlid A - Appl. Environ. Microbiol. (2015)

Analysis of secondary metabolites secreted by P. involutus during growth on organic matter. Shown are total ion current profiles from the HRESIMS (negative mode) spectra of the MH0 SPEtOAc eluate, recovered from the initial organic material (A), and of the MH7 SPEtOAc (B) and MH7 SPMeOH (C) fractions, sampled after 7 days of growth. Note that the MH7 SPEtOAc sample contains one major peak, indicated by the arrow (involutin), that is not present in the MH0 sample. Involutin is also present in MH7 SPMeOH. (Inset) HRESIMS profile of involutin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644656&req=5

Figure 2: Analysis of secondary metabolites secreted by P. involutus during growth on organic matter. Shown are total ion current profiles from the HRESIMS (negative mode) spectra of the MH0 SPEtOAc eluate, recovered from the initial organic material (A), and of the MH7 SPEtOAc (B) and MH7 SPMeOH (C) fractions, sampled after 7 days of growth. Note that the MH7 SPEtOAc sample contains one major peak, indicated by the arrow (involutin), that is not present in the MH0 sample. Involutin is also present in MH7 SPMeOH. (Inset) HRESIMS profile of involutin.
Mentions: LC-MS analysis of the MH0 SPEtOAc, MH7 SPEtOAc, and MH7 SPMeOH fractions revealed that one major compound was formed during the decomposition of the organic material (Fig. 2). Based on the HPLC and HRESIMS total-ion chromatogram, the signal corresponded to a single compound with m/z 313.0719 and thus was tentatively identified as involutin (Fig. 2B, inset). The total ion current profiles for the MH7 SPEtOAc and MH7 SPMeOH fractions were similar except that the peak associated with involutin was considerably lower in the MH7 SPMeOH fraction than in the MH7 SPEtOAc fraction (Fig. 2B and C).

Bottom Line: Activity-guided purification was performed to isolate the Fe(3+)-reducing principle secreted by P. involutus during growth on a maize compost extract.The Fe(3+)-reducing activity correlated with the presence of one compound.We also demonstrated that in the presence of H2O2, involutin has the capacity to drive an in vitro Fenton reaction via Fe(3+) reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Microbial Ecology Group, Lund University, Lund, Sweden Firoz.Shah@biol.lu.se.

No MeSH data available.


Related in: MedlinePlus