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Involutin is an Fe3+ reductant secreted by the ectomycorrhizal fungus Paxillus involutus during Fenton-based decomposition of organic matter.

Shah F, Schwenk D, Nicolás C, Persson P, Hoffmeister D, Tunlid A - Appl. Environ. Microbiol. (2015)

Bottom Line: Activity-guided purification was performed to isolate the Fe(3+)-reducing principle secreted by P. involutus during growth on a maize compost extract.The Fe(3+)-reducing activity correlated with the presence of one compound.We also demonstrated that in the presence of H2O2, involutin has the capacity to drive an in vitro Fenton reaction via Fe(3+) reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Microbial Ecology Group, Lund University, Lund, Sweden Firoz.Shah@biol.lu.se.

No MeSH data available.


Related in: MedlinePlus

Purification of Fe3+-reducing activity secreted by P. involutus during the decomposition of an organic matter (maize compost) extract. Shown are the concentrations of Fe2+ generated by the reduction of Fe3+ in samples collected at different steps of the purification procedure. The amounts of Fe2+ are normalized per milliliter of culture filtrate. Fe2+ generated by the mycelium extract is normalized per gram (dry weight) of biomass (1 g biomass is produced through ∼40 ml culture extract). Sample details: MMN7, culture filtrate collected after 7 days of growth on a synthetic mineral nutrient medium (MMN) (n = 3); MH0, culture filtrate collected before the fungus was added to the maize compost substrate (MH) (n = 3); MH7, culture filtrate collected after 7 days of growth on the MH substrate (n = 3); MYC, extract of the mycelium grown for 7 days on the MH substrate (n = 3). Error bars indicate standard errors. Thick arrows indicate the samples that were transferred to the next purification step. (A) Activities in the crude culture filtrates and the mycelium extract. (B) Ethyl acetate (EtOAc) phase extraction of the MH7 culture filtrate. (C) Solid-phase fractionation of the EtOAc phase eluted with cyclohexane (SPCyclohex), EtOAc (SPEtOAc), or methanol (SPMeOH). (D) HPLC-purified involutin from the MH7 SPEtOAc eluate.
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Figure 1: Purification of Fe3+-reducing activity secreted by P. involutus during the decomposition of an organic matter (maize compost) extract. Shown are the concentrations of Fe2+ generated by the reduction of Fe3+ in samples collected at different steps of the purification procedure. The amounts of Fe2+ are normalized per milliliter of culture filtrate. Fe2+ generated by the mycelium extract is normalized per gram (dry weight) of biomass (1 g biomass is produced through ∼40 ml culture extract). Sample details: MMN7, culture filtrate collected after 7 days of growth on a synthetic mineral nutrient medium (MMN) (n = 3); MH0, culture filtrate collected before the fungus was added to the maize compost substrate (MH) (n = 3); MH7, culture filtrate collected after 7 days of growth on the MH substrate (n = 3); MYC, extract of the mycelium grown for 7 days on the MH substrate (n = 3). Error bars indicate standard errors. Thick arrows indicate the samples that were transferred to the next purification step. (A) Activities in the crude culture filtrates and the mycelium extract. (B) Ethyl acetate (EtOAc) phase extraction of the MH7 culture filtrate. (C) Solid-phase fractionation of the EtOAc phase eluted with cyclohexane (SPCyclohex), EtOAc (SPEtOAc), or methanol (SPMeOH). (D) HPLC-purified involutin from the MH7 SPEtOAc eluate.

Mentions: P. involutus was grown on a maize compost (MH) substrate. Previous studies using spectroscopic analyses showed that this substrate is decomposed by the fungus using a brown rot mechanism involving Fenton chemistry (13). The production of Fe3+-reducing activity was analyzed both in the mycelium and in the culture filtrate (Fig. 1). At the start of the experiment, trace amounts of Fe3+-reducing activity were detected in the culture filtrate (MH0). After 7 days of incubation, high levels of Fe3+-reducing activity were detected in the culture filtrate (97% of the total activity) but only minute amounts in the mycelium (3% of the total activity). Fe3+-reducing activity was not detected in the mineral nutrient medium (MMN) incubated with the fungus for 7 days.


Involutin is an Fe3+ reductant secreted by the ectomycorrhizal fungus Paxillus involutus during Fenton-based decomposition of organic matter.

Shah F, Schwenk D, Nicolás C, Persson P, Hoffmeister D, Tunlid A - Appl. Environ. Microbiol. (2015)

Purification of Fe3+-reducing activity secreted by P. involutus during the decomposition of an organic matter (maize compost) extract. Shown are the concentrations of Fe2+ generated by the reduction of Fe3+ in samples collected at different steps of the purification procedure. The amounts of Fe2+ are normalized per milliliter of culture filtrate. Fe2+ generated by the mycelium extract is normalized per gram (dry weight) of biomass (1 g biomass is produced through ∼40 ml culture extract). Sample details: MMN7, culture filtrate collected after 7 days of growth on a synthetic mineral nutrient medium (MMN) (n = 3); MH0, culture filtrate collected before the fungus was added to the maize compost substrate (MH) (n = 3); MH7, culture filtrate collected after 7 days of growth on the MH substrate (n = 3); MYC, extract of the mycelium grown for 7 days on the MH substrate (n = 3). Error bars indicate standard errors. Thick arrows indicate the samples that were transferred to the next purification step. (A) Activities in the crude culture filtrates and the mycelium extract. (B) Ethyl acetate (EtOAc) phase extraction of the MH7 culture filtrate. (C) Solid-phase fractionation of the EtOAc phase eluted with cyclohexane (SPCyclohex), EtOAc (SPEtOAc), or methanol (SPMeOH). (D) HPLC-purified involutin from the MH7 SPEtOAc eluate.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 1: Purification of Fe3+-reducing activity secreted by P. involutus during the decomposition of an organic matter (maize compost) extract. Shown are the concentrations of Fe2+ generated by the reduction of Fe3+ in samples collected at different steps of the purification procedure. The amounts of Fe2+ are normalized per milliliter of culture filtrate. Fe2+ generated by the mycelium extract is normalized per gram (dry weight) of biomass (1 g biomass is produced through ∼40 ml culture extract). Sample details: MMN7, culture filtrate collected after 7 days of growth on a synthetic mineral nutrient medium (MMN) (n = 3); MH0, culture filtrate collected before the fungus was added to the maize compost substrate (MH) (n = 3); MH7, culture filtrate collected after 7 days of growth on the MH substrate (n = 3); MYC, extract of the mycelium grown for 7 days on the MH substrate (n = 3). Error bars indicate standard errors. Thick arrows indicate the samples that were transferred to the next purification step. (A) Activities in the crude culture filtrates and the mycelium extract. (B) Ethyl acetate (EtOAc) phase extraction of the MH7 culture filtrate. (C) Solid-phase fractionation of the EtOAc phase eluted with cyclohexane (SPCyclohex), EtOAc (SPEtOAc), or methanol (SPMeOH). (D) HPLC-purified involutin from the MH7 SPEtOAc eluate.
Mentions: P. involutus was grown on a maize compost (MH) substrate. Previous studies using spectroscopic analyses showed that this substrate is decomposed by the fungus using a brown rot mechanism involving Fenton chemistry (13). The production of Fe3+-reducing activity was analyzed both in the mycelium and in the culture filtrate (Fig. 1). At the start of the experiment, trace amounts of Fe3+-reducing activity were detected in the culture filtrate (MH0). After 7 days of incubation, high levels of Fe3+-reducing activity were detected in the culture filtrate (97% of the total activity) but only minute amounts in the mycelium (3% of the total activity). Fe3+-reducing activity was not detected in the mineral nutrient medium (MMN) incubated with the fungus for 7 days.

Bottom Line: Activity-guided purification was performed to isolate the Fe(3+)-reducing principle secreted by P. involutus during growth on a maize compost extract.The Fe(3+)-reducing activity correlated with the presence of one compound.We also demonstrated that in the presence of H2O2, involutin has the capacity to drive an in vitro Fenton reaction via Fe(3+) reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Microbial Ecology Group, Lund University, Lund, Sweden Firoz.Shah@biol.lu.se.

No MeSH data available.


Related in: MedlinePlus