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Effect of Genistein and L-Carnitine and Their Combination on Gene Expression of Hepatocyte HMG-COA Reductase and LDL Receptor in Experimental Nephrotic Syndrome.

Yousefinejad A, Siassi F, Mirshafiey A, Eshraghian MR, Koohdani F, Javanbakht MH, Sedaghat R, Ramezani A, Zarei M, Djalali M - Iran. J. Public Health (2015)

Bottom Line: L-carnitine and genistein can effect on lipid metabolism and the syndrome.The proteinuria and urine protein-to-creatinine ratio showed significant differences between PC group and LC, G and LCG groups at week 7 (P<0.001).ΔCT of LDLr mRNA showed significant differences between the PC group and the other patient groups (P<0.001).

View Article: PubMed Central - PubMed

Affiliation: Dept. of Nutrition, School of Public Health, Bushehr University of Medical Sciences, Bushehr, Iran.

ABSTRACT

Background: Nephrotic syndrome is a disorder that leads to hyperlipidemia. L-carnitine and genistein can effect on lipid metabolism and the syndrome. In the present study, we have delved into the separate and the twin-effects of L-carnitine and genistein on the gene expressions of HMG-COA reductase and LDL receptor in experimental nephrotic syndrome.

Methods: In this controlled experimental study, 50 male Sprague-Dawley rats were randomly divided into five groups: NC (normal-control), PC (patient-control), LC (L-carnitine), G (genistein), LCG (L-carnitine-genistein). Adriamycin was used for inducing nephrotic syndrome and the spot urine samples and urine protein-to-creatinine ratio were measured. Hepatocytic RNA was extracted and real-time PCR was used for HMG-COA Reductase and LDL receptor gene Expression measurement.

Results: The final weight of the patients groups were lower than the NC group (P=0.001), and weight gain of the NC group was higher than the other groups (P<0.001). The proteinuria and urine protein-to-creatinine ratio showed significant differences between PC group and LC, G and LCG groups at week 7 (P<0.001). The expression of HMGCOA Reductase mRNA down regulated in LC, G and LCG groups in comparison with PC group (P<0.001). ΔCT of LDLr mRNA showed significant differences between the PC group and the other patient groups (P<0.001).

Conclusion: This study shows a significant decreasing (P<0.001) and non-significant increasing trend in HMG-COA Reductase and LDLr gene expression, respectively, and synergistic effect of L-carnitine and genistein on these genes in experimental nephrotic syndrome.

No MeSH data available.


Related in: MedlinePlus

NanoDrop spectrophotometer of the extracted RNA
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Figure 1: NanoDrop spectrophotometer of the extracted RNA

Mentions: Cytoplasmic RNA was extracted and purified using RNeasy Plus Mini Kit (Qiagen, Valencia, Calif., USA) according to the manufacturer’s protocol. Quantity and purity of extracted RNA was checked by NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, Del., USA). A ratio of A 260/280 between 1.9–2.1 was taken into account as pure RNA (Fig. 1). Single-strand cDNA was synthesized using QuantiTect Reverse Transcription Kit (Qiagen).


Effect of Genistein and L-Carnitine and Their Combination on Gene Expression of Hepatocyte HMG-COA Reductase and LDL Receptor in Experimental Nephrotic Syndrome.

Yousefinejad A, Siassi F, Mirshafiey A, Eshraghian MR, Koohdani F, Javanbakht MH, Sedaghat R, Ramezani A, Zarei M, Djalali M - Iran. J. Public Health (2015)

NanoDrop spectrophotometer of the extracted RNA
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644578&req=5

Figure 1: NanoDrop spectrophotometer of the extracted RNA
Mentions: Cytoplasmic RNA was extracted and purified using RNeasy Plus Mini Kit (Qiagen, Valencia, Calif., USA) according to the manufacturer’s protocol. Quantity and purity of extracted RNA was checked by NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, Del., USA). A ratio of A 260/280 between 1.9–2.1 was taken into account as pure RNA (Fig. 1). Single-strand cDNA was synthesized using QuantiTect Reverse Transcription Kit (Qiagen).

Bottom Line: L-carnitine and genistein can effect on lipid metabolism and the syndrome.The proteinuria and urine protein-to-creatinine ratio showed significant differences between PC group and LC, G and LCG groups at week 7 (P<0.001).ΔCT of LDLr mRNA showed significant differences between the PC group and the other patient groups (P<0.001).

View Article: PubMed Central - PubMed

Affiliation: Dept. of Nutrition, School of Public Health, Bushehr University of Medical Sciences, Bushehr, Iran.

ABSTRACT

Background: Nephrotic syndrome is a disorder that leads to hyperlipidemia. L-carnitine and genistein can effect on lipid metabolism and the syndrome. In the present study, we have delved into the separate and the twin-effects of L-carnitine and genistein on the gene expressions of HMG-COA reductase and LDL receptor in experimental nephrotic syndrome.

Methods: In this controlled experimental study, 50 male Sprague-Dawley rats were randomly divided into five groups: NC (normal-control), PC (patient-control), LC (L-carnitine), G (genistein), LCG (L-carnitine-genistein). Adriamycin was used for inducing nephrotic syndrome and the spot urine samples and urine protein-to-creatinine ratio were measured. Hepatocytic RNA was extracted and real-time PCR was used for HMG-COA Reductase and LDL receptor gene Expression measurement.

Results: The final weight of the patients groups were lower than the NC group (P=0.001), and weight gain of the NC group was higher than the other groups (P<0.001). The proteinuria and urine protein-to-creatinine ratio showed significant differences between PC group and LC, G and LCG groups at week 7 (P<0.001). The expression of HMGCOA Reductase mRNA down regulated in LC, G and LCG groups in comparison with PC group (P<0.001). ΔCT of LDLr mRNA showed significant differences between the PC group and the other patient groups (P<0.001).

Conclusion: This study shows a significant decreasing (P<0.001) and non-significant increasing trend in HMG-COA Reductase and LDLr gene expression, respectively, and synergistic effect of L-carnitine and genistein on these genes in experimental nephrotic syndrome.

No MeSH data available.


Related in: MedlinePlus