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The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of Rabbit.

Mehrabani D, Babazadeh M, Tanideh N, Zare S, Hoseinzadeh S, Torabinejad S, Koohi-Hosseinabadi O - Int J Organ Transplant Med (2015)

Bottom Line: Neo-formed tissues in cell-treated groups were translucent with a smooth and intact surface and less irregularity.In cell-treated group after 8 weeks post-transplantation, the overall healing score of experimental knees were superior when compared to other groups.We showed that Ad-MSCs, as an available and non-invasive produced source of cells, could be safely administered in knee osteochondral defects.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Background: Articular cartilage defect can lead to degradation of subchondral bone and osteoarthritis (OA).

Objective: To determine the healing effect of transplantation of adipose-derived mesenchymal stem cells (Ad-MSCs) in full-thickness femoral articular cartilage defects in rabbit.

Methods: 12 rabbits were equally divided into cell-treated and control groups. In cell-treated group, 2×10(6) cells of third passage suspended in 1 mL of DMEM was injected into articular defect. The control group just received 1 mL of DMEM. Dulbecco's modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin and streptomycin and 2 mM L-glutamine were used for cell culture. To induce cartilage defect, 4 mm articular cartilage full-thickness defect was created in the knee. For histological evaluation in each group (H&E, safranin-O and toluidine blue), 3 rabbits were sacrificed 4 weeks and 3 animals, 8 weeks after cell transplantation.

Results: In cell therapy group post-transplantation, no abnormal gross findings were noticed. Neo-formed tissues in cell-treated groups were translucent with a smooth and intact surface and less irregularity. In cell-treated group after 8 weeks post-transplantation, the overall healing score of experimental knees were superior when compared to other groups.

Conclusion: We showed that Ad-MSCs, as an available and non-invasive produced source of cells, could be safely administered in knee osteochondral defects.

No MeSH data available.


Related in: MedlinePlus

From left to right; 1st (A), 2nd (B) and 3rd passage (C) of Ad-MSCs of rabbit (×40).
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Figure 1: From left to right; 1st (A), 2nd (B) and 3rd passage (C) of Ad-MSCs of rabbit (×40).

Mentions: Fibroblastic adherent cells were seen in the culture flask and in all subsequent subcultures (Fig 1). It was noticed that all cells were positive for expression of CD73 and negative for CD45 by RT-PCR (Fig 2). At 4 and 8 weeks post-transplantation, no rejection, infection, presence of severe inflammation, fibrosis, discoloration, irregularity, depression or bulging of repaired tissues were seen. Neo-formed tissues in transplanted cell group were translucent with a smooth and intact surface and less irregularity. In the control group receiving no cells, a depression in the defect area was visible. The articular surfaces in the defect site of transplanted cell group were relatively smoother than in the control group and their coloration was closer to that of surrounding normal cartilage in comparison to the control knees. Furthermore, in transplanted cell group; the border areas of defects were less distinct and depressions were less obvious than the control knees.


The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of Rabbit.

Mehrabani D, Babazadeh M, Tanideh N, Zare S, Hoseinzadeh S, Torabinejad S, Koohi-Hosseinabadi O - Int J Organ Transplant Med (2015)

From left to right; 1st (A), 2nd (B) and 3rd passage (C) of Ad-MSCs of rabbit (×40).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644569&req=5

Figure 1: From left to right; 1st (A), 2nd (B) and 3rd passage (C) of Ad-MSCs of rabbit (×40).
Mentions: Fibroblastic adherent cells were seen in the culture flask and in all subsequent subcultures (Fig 1). It was noticed that all cells were positive for expression of CD73 and negative for CD45 by RT-PCR (Fig 2). At 4 and 8 weeks post-transplantation, no rejection, infection, presence of severe inflammation, fibrosis, discoloration, irregularity, depression or bulging of repaired tissues were seen. Neo-formed tissues in transplanted cell group were translucent with a smooth and intact surface and less irregularity. In the control group receiving no cells, a depression in the defect area was visible. The articular surfaces in the defect site of transplanted cell group were relatively smoother than in the control group and their coloration was closer to that of surrounding normal cartilage in comparison to the control knees. Furthermore, in transplanted cell group; the border areas of defects were less distinct and depressions were less obvious than the control knees.

Bottom Line: Neo-formed tissues in cell-treated groups were translucent with a smooth and intact surface and less irregularity.In cell-treated group after 8 weeks post-transplantation, the overall healing score of experimental knees were superior when compared to other groups.We showed that Ad-MSCs, as an available and non-invasive produced source of cells, could be safely administered in knee osteochondral defects.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Background: Articular cartilage defect can lead to degradation of subchondral bone and osteoarthritis (OA).

Objective: To determine the healing effect of transplantation of adipose-derived mesenchymal stem cells (Ad-MSCs) in full-thickness femoral articular cartilage defects in rabbit.

Methods: 12 rabbits were equally divided into cell-treated and control groups. In cell-treated group, 2×10(6) cells of third passage suspended in 1 mL of DMEM was injected into articular defect. The control group just received 1 mL of DMEM. Dulbecco's modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin and streptomycin and 2 mM L-glutamine were used for cell culture. To induce cartilage defect, 4 mm articular cartilage full-thickness defect was created in the knee. For histological evaluation in each group (H&E, safranin-O and toluidine blue), 3 rabbits were sacrificed 4 weeks and 3 animals, 8 weeks after cell transplantation.

Results: In cell therapy group post-transplantation, no abnormal gross findings were noticed. Neo-formed tissues in cell-treated groups were translucent with a smooth and intact surface and less irregularity. In cell-treated group after 8 weeks post-transplantation, the overall healing score of experimental knees were superior when compared to other groups.

Conclusion: We showed that Ad-MSCs, as an available and non-invasive produced source of cells, could be safely administered in knee osteochondral defects.

No MeSH data available.


Related in: MedlinePlus