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HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene.

Vallinoto AC, Santana BB, Sá KS, Ferreira TC, Sousa RC, Azevedo VN, Feitosa RN, Machado LF, Ishak MO, Ishak R - Mediators Inflamm. (2015)

Bottom Line: The allelic and genotypic distributions displayed no significant differences among the investigated groups.The proviral load was significantly higher in HAM/TSP patients than in asymptomatic subjects.The present results do not support a previous report indicating an association between the SNP rs12979860 and HAM/TSP outcome.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.

ABSTRACT
The present study investigated the association between the rs12979860 polymorphism in the IL-28B gene and HTLV-1 infection as well as the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1-infected patients (26 HAM/TSP symptomatic and 53 asymptomatic) and 300 seronegative healthy controls were investigated. Plasma levels of the cytokines TNF-α, TNF-β, IL-8, IL-10, IL-6, and IFN-γ from infected patients were measured using an indirect enzyme-linked immunosorbent assay. The HTLV proviral load was measured using a real-time PCR assay, and T-cell subset counts were determined by flow cytometry. Real-time PCR was used to genotype the rs12979860 SNP. The allelic and genotypic distributions displayed no significant differences among the investigated groups. No significant association between the serum cytokine levels and the presence of the rs12979860 SNP in symptomatic and asymptomatic subjects was observed. A positive correlation (p = 0.0015) between TNF-β and IFN-γ was observed in the asymptomatic group, but a positive correlation was only observed (p = 0.0180) between TNF-α and IL-6 in the HAM/TSP group. The proviral load was significantly higher in HAM/TSP patients than in asymptomatic subjects. The present results do not support a previous report indicating an association between the SNP rs12979860 and HAM/TSP outcome.

No MeSH data available.


Related in: MedlinePlus

Cytokine serum levels according to the presence of the SNP rs12979860 in the HAM/TSP and asymptomatic groups.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig1: Cytokine serum levels according to the presence of the SNP rs12979860 in the HAM/TSP and asymptomatic groups.

Mentions: No significant association (Figure 1) was observed between cytokine levels and the presence of rs12979860 (genotypes CT+TT). However, when the cytokine levels were measured according to the presence or absence of HAM/TSP symptoms (Figure 2), a significantly increased level of TNF-α was observed in the HAM/TSP patients. In an attempt to correlate each cytokine value with each other (Figure 2), a positive correlation (p = 0.0013; r2 = 0.3965) between TNF-β and IFN-γ was observed in the asymptomatic group, whereas TNF-α and IL-6 (p = 0.0162; r2 = 0.3944) as well as IL-10 and IL-8 (p = 0.0207; r2 = 0.3476) displayed positive correlations in the HAM/TSP group.


HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene.

Vallinoto AC, Santana BB, Sá KS, Ferreira TC, Sousa RC, Azevedo VN, Feitosa RN, Machado LF, Ishak MO, Ishak R - Mediators Inflamm. (2015)

Cytokine serum levels according to the presence of the SNP rs12979860 in the HAM/TSP and asymptomatic groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644563&req=5

fig1: Cytokine serum levels according to the presence of the SNP rs12979860 in the HAM/TSP and asymptomatic groups.
Mentions: No significant association (Figure 1) was observed between cytokine levels and the presence of rs12979860 (genotypes CT+TT). However, when the cytokine levels were measured according to the presence or absence of HAM/TSP symptoms (Figure 2), a significantly increased level of TNF-α was observed in the HAM/TSP patients. In an attempt to correlate each cytokine value with each other (Figure 2), a positive correlation (p = 0.0013; r2 = 0.3965) between TNF-β and IFN-γ was observed in the asymptomatic group, whereas TNF-α and IL-6 (p = 0.0162; r2 = 0.3944) as well as IL-10 and IL-8 (p = 0.0207; r2 = 0.3476) displayed positive correlations in the HAM/TSP group.

Bottom Line: The allelic and genotypic distributions displayed no significant differences among the investigated groups.The proviral load was significantly higher in HAM/TSP patients than in asymptomatic subjects.The present results do not support a previous report indicating an association between the SNP rs12979860 and HAM/TSP outcome.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.

ABSTRACT
The present study investigated the association between the rs12979860 polymorphism in the IL-28B gene and HTLV-1 infection as well as the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1-infected patients (26 HAM/TSP symptomatic and 53 asymptomatic) and 300 seronegative healthy controls were investigated. Plasma levels of the cytokines TNF-α, TNF-β, IL-8, IL-10, IL-6, and IFN-γ from infected patients were measured using an indirect enzyme-linked immunosorbent assay. The HTLV proviral load was measured using a real-time PCR assay, and T-cell subset counts were determined by flow cytometry. Real-time PCR was used to genotype the rs12979860 SNP. The allelic and genotypic distributions displayed no significant differences among the investigated groups. No significant association between the serum cytokine levels and the presence of the rs12979860 SNP in symptomatic and asymptomatic subjects was observed. A positive correlation (p = 0.0015) between TNF-β and IFN-γ was observed in the asymptomatic group, but a positive correlation was only observed (p = 0.0180) between TNF-α and IL-6 in the HAM/TSP group. The proviral load was significantly higher in HAM/TSP patients than in asymptomatic subjects. The present results do not support a previous report indicating an association between the SNP rs12979860 and HAM/TSP outcome.

No MeSH data available.


Related in: MedlinePlus