Limits...
The Combination of Resveratrol and Quercetin Attenuates Metabolic Syndrome in Rats by Modifying the Serum Fatty Acid Composition and by Upregulating SIRT 1 and SIRT 2 Expression in White Adipose Tissue.

Peredo-Escárcega AE, Guarner-Lans V, Pérez-Torres I, Ortega-Ocampo S, Carreón-Torres E, Castrejón-Tellez V, Díaz-Díaz E, Rubio-Ruiz ME - Evid Based Complement Alternat Med (2015)

Bottom Line: MetS was induced in Wistar rats by adding 30% sucrose to drinking water for five months.Rats were divided into control and two groups receiving the two different doses of RSV and QRC in drinking water daily for 4 weeks following the 5 months of sucrose treatment.In MetS rats body mass, central adiposity, insulin, triglycerides, non-HDL-C, leptin, adiponectin, monounsaturated fatty acids (MUFAs), and nonesterified fatty acids (NEFAs) were increased, while polyunsaturated fatty acids (PUFAs) and HDL-C were decreased.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Instituto Nacional de Cardiología "Ignacio Chávez", 14080 Mexico City, Mexico.

ABSTRACT
Resveratrol (RSV) and quercetin (QRC) modify energy metabolism and reduce cardiovascular risk factors included in the metabolic syndrome (MetS). These natural compounds upregulate and activate sirtuins (SIRTs), a family of NAD-dependent histone deacetylases. We analyzed the effect of two doses of a commercial combination of RSV and QRC on serum fatty acid composition and their regulation of SIRTs 1-3 and PPAR-γ expression in white adipose tissue. MetS was induced in Wistar rats by adding 30% sucrose to drinking water for five months. Rats were divided into control and two groups receiving the two different doses of RSV and QRC in drinking water daily for 4 weeks following the 5 months of sucrose treatment. Commercial kits were used to determine serum parameters and the expressions of SIRTs in WAT were analysed by western blot. In MetS rats body mass, central adiposity, insulin, triglycerides, non-HDL-C, leptin, adiponectin, monounsaturated fatty acids (MUFAs), and nonesterified fatty acids (NEFAs) were increased, while polyunsaturated fatty acids (PUFAs) and HDL-C were decreased. SIRT 1 and SIRT 2 were downregulated, while PPAR-γ was increased. RSV + QRC administration improved the serum health parameters modified by MetS and upregulate SIRT 1 and SIRT 2 expression in white abdominal tissue in MetS animals.

No MeSH data available.


Related in: MedlinePlus

RSV plus QRC leads to SIRT 1 expression in WAT from MetS rats. (a) Protein expression, data represent mean ± SEM (n = 6 per group). #P < 0.05; ∗P < 0.05 against MetS without treatment. (b) Representative Western blot analysis. Line 1: control without treatment; line 2: control treated with RSV 10 + QRC 0.19; line 3: control treated with RSV 50 + QRC 0.95; line 4, MetS without treatment; line 5: MetS treated with RSV 10 + QRC 0.19; line 6: MetS treated with RSV 50 + QRC 0.95.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4644561&req=5

fig1: RSV plus QRC leads to SIRT 1 expression in WAT from MetS rats. (a) Protein expression, data represent mean ± SEM (n = 6 per group). #P < 0.05; ∗P < 0.05 against MetS without treatment. (b) Representative Western blot analysis. Line 1: control without treatment; line 2: control treated with RSV 10 + QRC 0.19; line 3: control treated with RSV 50 + QRC 0.95; line 4, MetS without treatment; line 5: MetS treated with RSV 10 + QRC 0.19; line 6: MetS treated with RSV 50 + QRC 0.95.

Mentions: The data in Figures 1, 3, and 4 show SIRT 1 (62 KDa), SIRT 2 (43 KDa), and SIRT 3 (28 KDa) levels, respectively, of control and MetS rats treated with RSV + QRC. The expression of SIRT 1 and SIRT 2 in MetS rats was reduced when compared to control rats. When assessing the effect of RSV + QRC treatment in MetS group, we observed a clear tendency towards increased values of SIRT 1 expression with the lowest dose and a significant increase with RSV 50 + QRC 0.95 (Figures 1(a) and 1(b)). There was a significant increase in SIRT 2 expression with both doses of RSV + QRC (Figures 3(a) and 3(b)). There was no significant change in SIRT 1 and SIRT 2 expression in control rats treated with RSV + QRC.


The Combination of Resveratrol and Quercetin Attenuates Metabolic Syndrome in Rats by Modifying the Serum Fatty Acid Composition and by Upregulating SIRT 1 and SIRT 2 Expression in White Adipose Tissue.

Peredo-Escárcega AE, Guarner-Lans V, Pérez-Torres I, Ortega-Ocampo S, Carreón-Torres E, Castrejón-Tellez V, Díaz-Díaz E, Rubio-Ruiz ME - Evid Based Complement Alternat Med (2015)

RSV plus QRC leads to SIRT 1 expression in WAT from MetS rats. (a) Protein expression, data represent mean ± SEM (n = 6 per group). #P < 0.05; ∗P < 0.05 against MetS without treatment. (b) Representative Western blot analysis. Line 1: control without treatment; line 2: control treated with RSV 10 + QRC 0.19; line 3: control treated with RSV 50 + QRC 0.95; line 4, MetS without treatment; line 5: MetS treated with RSV 10 + QRC 0.19; line 6: MetS treated with RSV 50 + QRC 0.95.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644561&req=5

fig1: RSV plus QRC leads to SIRT 1 expression in WAT from MetS rats. (a) Protein expression, data represent mean ± SEM (n = 6 per group). #P < 0.05; ∗P < 0.05 against MetS without treatment. (b) Representative Western blot analysis. Line 1: control without treatment; line 2: control treated with RSV 10 + QRC 0.19; line 3: control treated with RSV 50 + QRC 0.95; line 4, MetS without treatment; line 5: MetS treated with RSV 10 + QRC 0.19; line 6: MetS treated with RSV 50 + QRC 0.95.
Mentions: The data in Figures 1, 3, and 4 show SIRT 1 (62 KDa), SIRT 2 (43 KDa), and SIRT 3 (28 KDa) levels, respectively, of control and MetS rats treated with RSV + QRC. The expression of SIRT 1 and SIRT 2 in MetS rats was reduced when compared to control rats. When assessing the effect of RSV + QRC treatment in MetS group, we observed a clear tendency towards increased values of SIRT 1 expression with the lowest dose and a significant increase with RSV 50 + QRC 0.95 (Figures 1(a) and 1(b)). There was a significant increase in SIRT 2 expression with both doses of RSV + QRC (Figures 3(a) and 3(b)). There was no significant change in SIRT 1 and SIRT 2 expression in control rats treated with RSV + QRC.

Bottom Line: MetS was induced in Wistar rats by adding 30% sucrose to drinking water for five months.Rats were divided into control and two groups receiving the two different doses of RSV and QRC in drinking water daily for 4 weeks following the 5 months of sucrose treatment.In MetS rats body mass, central adiposity, insulin, triglycerides, non-HDL-C, leptin, adiponectin, monounsaturated fatty acids (MUFAs), and nonesterified fatty acids (NEFAs) were increased, while polyunsaturated fatty acids (PUFAs) and HDL-C were decreased.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Instituto Nacional de Cardiología "Ignacio Chávez", 14080 Mexico City, Mexico.

ABSTRACT
Resveratrol (RSV) and quercetin (QRC) modify energy metabolism and reduce cardiovascular risk factors included in the metabolic syndrome (MetS). These natural compounds upregulate and activate sirtuins (SIRTs), a family of NAD-dependent histone deacetylases. We analyzed the effect of two doses of a commercial combination of RSV and QRC on serum fatty acid composition and their regulation of SIRTs 1-3 and PPAR-γ expression in white adipose tissue. MetS was induced in Wistar rats by adding 30% sucrose to drinking water for five months. Rats were divided into control and two groups receiving the two different doses of RSV and QRC in drinking water daily for 4 weeks following the 5 months of sucrose treatment. Commercial kits were used to determine serum parameters and the expressions of SIRTs in WAT were analysed by western blot. In MetS rats body mass, central adiposity, insulin, triglycerides, non-HDL-C, leptin, adiponectin, monounsaturated fatty acids (MUFAs), and nonesterified fatty acids (NEFAs) were increased, while polyunsaturated fatty acids (PUFAs) and HDL-C were decreased. SIRT 1 and SIRT 2 were downregulated, while PPAR-γ was increased. RSV + QRC administration improved the serum health parameters modified by MetS and upregulate SIRT 1 and SIRT 2 expression in white abdominal tissue in MetS animals.

No MeSH data available.


Related in: MedlinePlus