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Separation of Berberine Hydrochloride and Tetrahydropalmatine and Their Quantitative Analysis with Thin Layer Chromatography Involved with Ionic Liquids.

Lu J, Ma HY, Zhang W, Ma ZG, Yao S - J Anal Methods Chem (2015)

Bottom Line: [BMIM]OH was used in mobile and stationary phase of thin layer chromatography (TLC) to analyze berberine hydrochloride and tetrahydropalmatine for the first time.Moreover, on the plates prepared by SiO2·Im(+)·OH(-), the contents of the above alkaloids in the Chinese patent medicine (CPM) of "Stomacheasy" capsule were successfully determined by TLC scanner.This study is expected to be helpful in expanding the application of IL and its bonded silica gel in TLC separation field.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

ABSTRACT
[BMIM]OH was used in mobile and stationary phase of thin layer chromatography (TLC) to analyze berberine hydrochloride and tetrahydropalmatine for the first time. Supported imidazole ionic liquid with hydroxide ion on silica gel (SiO2·Im(+)·OH(-)) was synthesized through simple procedure and characterized by Fourier transform infrared spectroscopy (FT-IR), elemental analysis, and scanning electron microscope (SEM). Moreover, on the plates prepared by SiO2·Im(+)·OH(-), the contents of the above alkaloids in the Chinese patent medicine (CPM) of "Stomacheasy" capsule were successfully determined by TLC scanner. The key conditions and chromatographic behaviors were also investigated in detail. According to similar ways, ionic liquids (ILs) also can be used in other planar chromatographies in two modes. This study is expected to be helpful in expanding the application of IL and its bonded silica gel in TLC separation field.

No MeSH data available.


Related in: MedlinePlus

IR spectrum of blank silica gel (a) and SIL (b) together with SEM of SIL (c).
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fig4: IR spectrum of blank silica gel (a) and SIL (b) together with SEM of SIL (c).

Mentions: The elemental contents of SiO2·Im+·OH− final product were analyzed, and the content of ionic liquid attached to the silica surface was obtained as 1.072 mmol/g. From the SEM photo (×1500, Figure 4), it can be seen that the surface of SiO2·Im+·OH− particles is relatively smooth, which is different from the surface of common amorphous silica powders with many grooves and pores. A thermogravimetric study was performed to evaluate the thermal stability of SiO2·Im+·OH−, and its TGA curve only showed an initial loss of weight at temperature below 200°C, which was attributed to the removal of physically adsorbed water. Moreover, infrared spectrometry is one of the useful tools to identify the chemical modifications of silica gel. Small differences in wave numbers and intensities of the absorption bands are observed in the spectra of activated silica and immobilized ILs. In this study, FT-IR spectra of the activated silica and SILs were recorded between 400 cm−1 and 4000 cm−1, which were shown in Figures 4(a) and 4(b). In the spectrum of anchored [BMIM]OH surface, the finger-print region of the amide bands was from 1500 to 1600 cm−1; 1576 cm−1 is attributed to the characteristic frequency of the imidazolium groups, and 2974 cm−1 is assigned to the C-H stretching of the tetrahedral carbon. These IR peaks are similar to those reported in previous references [7, 21] and no obvious change of IR spectrum was observed in dry and sealed environment within 1 week. The above results can confirm the successful and stable anchoring of the IL onto the silica surface.


Separation of Berberine Hydrochloride and Tetrahydropalmatine and Their Quantitative Analysis with Thin Layer Chromatography Involved with Ionic Liquids.

Lu J, Ma HY, Zhang W, Ma ZG, Yao S - J Anal Methods Chem (2015)

IR spectrum of blank silica gel (a) and SIL (b) together with SEM of SIL (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644556&req=5

fig4: IR spectrum of blank silica gel (a) and SIL (b) together with SEM of SIL (c).
Mentions: The elemental contents of SiO2·Im+·OH− final product were analyzed, and the content of ionic liquid attached to the silica surface was obtained as 1.072 mmol/g. From the SEM photo (×1500, Figure 4), it can be seen that the surface of SiO2·Im+·OH− particles is relatively smooth, which is different from the surface of common amorphous silica powders with many grooves and pores. A thermogravimetric study was performed to evaluate the thermal stability of SiO2·Im+·OH−, and its TGA curve only showed an initial loss of weight at temperature below 200°C, which was attributed to the removal of physically adsorbed water. Moreover, infrared spectrometry is one of the useful tools to identify the chemical modifications of silica gel. Small differences in wave numbers and intensities of the absorption bands are observed in the spectra of activated silica and immobilized ILs. In this study, FT-IR spectra of the activated silica and SILs were recorded between 400 cm−1 and 4000 cm−1, which were shown in Figures 4(a) and 4(b). In the spectrum of anchored [BMIM]OH surface, the finger-print region of the amide bands was from 1500 to 1600 cm−1; 1576 cm−1 is attributed to the characteristic frequency of the imidazolium groups, and 2974 cm−1 is assigned to the C-H stretching of the tetrahedral carbon. These IR peaks are similar to those reported in previous references [7, 21] and no obvious change of IR spectrum was observed in dry and sealed environment within 1 week. The above results can confirm the successful and stable anchoring of the IL onto the silica surface.

Bottom Line: [BMIM]OH was used in mobile and stationary phase of thin layer chromatography (TLC) to analyze berberine hydrochloride and tetrahydropalmatine for the first time.Moreover, on the plates prepared by SiO2·Im(+)·OH(-), the contents of the above alkaloids in the Chinese patent medicine (CPM) of "Stomacheasy" capsule were successfully determined by TLC scanner.This study is expected to be helpful in expanding the application of IL and its bonded silica gel in TLC separation field.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

ABSTRACT
[BMIM]OH was used in mobile and stationary phase of thin layer chromatography (TLC) to analyze berberine hydrochloride and tetrahydropalmatine for the first time. Supported imidazole ionic liquid with hydroxide ion on silica gel (SiO2·Im(+)·OH(-)) was synthesized through simple procedure and characterized by Fourier transform infrared spectroscopy (FT-IR), elemental analysis, and scanning electron microscope (SEM). Moreover, on the plates prepared by SiO2·Im(+)·OH(-), the contents of the above alkaloids in the Chinese patent medicine (CPM) of "Stomacheasy" capsule were successfully determined by TLC scanner. The key conditions and chromatographic behaviors were also investigated in detail. According to similar ways, ionic liquids (ILs) also can be used in other planar chromatographies in two modes. This study is expected to be helpful in expanding the application of IL and its bonded silica gel in TLC separation field.

No MeSH data available.


Related in: MedlinePlus