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Separation of Berberine Hydrochloride and Tetrahydropalmatine and Their Quantitative Analysis with Thin Layer Chromatography Involved with Ionic Liquids.

Lu J, Ma HY, Zhang W, Ma ZG, Yao S - J Anal Methods Chem (2015)

Bottom Line: [BMIM]OH was used in mobile and stationary phase of thin layer chromatography (TLC) to analyze berberine hydrochloride and tetrahydropalmatine for the first time.Moreover, on the plates prepared by SiO2·Im(+)·OH(-), the contents of the above alkaloids in the Chinese patent medicine (CPM) of "Stomacheasy" capsule were successfully determined by TLC scanner.This study is expected to be helpful in expanding the application of IL and its bonded silica gel in TLC separation field.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

ABSTRACT
[BMIM]OH was used in mobile and stationary phase of thin layer chromatography (TLC) to analyze berberine hydrochloride and tetrahydropalmatine for the first time. Supported imidazole ionic liquid with hydroxide ion on silica gel (SiO2·Im(+)·OH(-)) was synthesized through simple procedure and characterized by Fourier transform infrared spectroscopy (FT-IR), elemental analysis, and scanning electron microscope (SEM). Moreover, on the plates prepared by SiO2·Im(+)·OH(-), the contents of the above alkaloids in the Chinese patent medicine (CPM) of "Stomacheasy" capsule were successfully determined by TLC scanner. The key conditions and chromatographic behaviors were also investigated in detail. According to similar ways, ionic liquids (ILs) also can be used in other planar chromatographies in two modes. This study is expected to be helpful in expanding the application of IL and its bonded silica gel in TLC separation field.

No MeSH data available.


Related in: MedlinePlus

Effect of [BMIM]OH concentration on Rf values of berberine hydrochloride.
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Related In: Results  -  Collection


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fig2: Effect of [BMIM]OH concentration on Rf values of berberine hydrochloride.

Mentions: Moreover, [BMIM]OH is a kind of basic ionic liquid compared with the other three neutral ones. A part of its effects is supposed to be similar to the effect of amines as pH adjuster in those popular multicomponent developing reagents for berberine hydrochloride (like ammonia in the system of “benzene-ethyl acetate-methanol-isopropanol-strong ammonia (12 : 6 : 3 : 3 : 1, V/V)” or diethylamine in “ethyl acetate-chloroform-methanol-diethylamine (8 : 2 : 2 : 1, V/V)”) [17, 20]. Meanwhile, the amount of IL would obviously influence the migration distance of the alkaloids (see Figure 2). The result proves the proportional relationship of Rf value of berberine hydrochloride and [BMIM]OH concentration, and IL plays a more important key role in the developing system than methanol.


Separation of Berberine Hydrochloride and Tetrahydropalmatine and Their Quantitative Analysis with Thin Layer Chromatography Involved with Ionic Liquids.

Lu J, Ma HY, Zhang W, Ma ZG, Yao S - J Anal Methods Chem (2015)

Effect of [BMIM]OH concentration on Rf values of berberine hydrochloride.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644556&req=5

fig2: Effect of [BMIM]OH concentration on Rf values of berberine hydrochloride.
Mentions: Moreover, [BMIM]OH is a kind of basic ionic liquid compared with the other three neutral ones. A part of its effects is supposed to be similar to the effect of amines as pH adjuster in those popular multicomponent developing reagents for berberine hydrochloride (like ammonia in the system of “benzene-ethyl acetate-methanol-isopropanol-strong ammonia (12 : 6 : 3 : 3 : 1, V/V)” or diethylamine in “ethyl acetate-chloroform-methanol-diethylamine (8 : 2 : 2 : 1, V/V)”) [17, 20]. Meanwhile, the amount of IL would obviously influence the migration distance of the alkaloids (see Figure 2). The result proves the proportional relationship of Rf value of berberine hydrochloride and [BMIM]OH concentration, and IL plays a more important key role in the developing system than methanol.

Bottom Line: [BMIM]OH was used in mobile and stationary phase of thin layer chromatography (TLC) to analyze berberine hydrochloride and tetrahydropalmatine for the first time.Moreover, on the plates prepared by SiO2·Im(+)·OH(-), the contents of the above alkaloids in the Chinese patent medicine (CPM) of "Stomacheasy" capsule were successfully determined by TLC scanner.This study is expected to be helpful in expanding the application of IL and its bonded silica gel in TLC separation field.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

ABSTRACT
[BMIM]OH was used in mobile and stationary phase of thin layer chromatography (TLC) to analyze berberine hydrochloride and tetrahydropalmatine for the first time. Supported imidazole ionic liquid with hydroxide ion on silica gel (SiO2·Im(+)·OH(-)) was synthesized through simple procedure and characterized by Fourier transform infrared spectroscopy (FT-IR), elemental analysis, and scanning electron microscope (SEM). Moreover, on the plates prepared by SiO2·Im(+)·OH(-), the contents of the above alkaloids in the Chinese patent medicine (CPM) of "Stomacheasy" capsule were successfully determined by TLC scanner. The key conditions and chromatographic behaviors were also investigated in detail. According to similar ways, ionic liquids (ILs) also can be used in other planar chromatographies in two modes. This study is expected to be helpful in expanding the application of IL and its bonded silica gel in TLC separation field.

No MeSH data available.


Related in: MedlinePlus