Limits...
Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability.

Mundargi R, Venkataraman D, Kumar S, Mogal V, Ortiz R, Loo J, Venkatraman S, Steele T - Biomed Res Int (2015)

Bottom Line: The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue.Histological evaluations with hematoxylin and eosin and Masson's trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements.Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.

View Article: PubMed Central - PubMed

Affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798.

ABSTRACT
The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue. Porcine carotid artery as a model tissue was used in the ex vivo bioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure, temperature, and flow. The real time tissue viability was evaluated by monitoring tissue metabolism through a fluorescent indicator "resorufin." Our ex vivo bioreactor allows real time monitoring of tissue responses along with physiological conditions. These ex vivo parameters were vital in determining the tissue viability in sensor-enabled bioreactor and our initial investigations suggest that, porcine tissue viability is considerably affected by high shear forces and low oxygen levels. Histological evaluations with hematoxylin and eosin and Masson's trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements. Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.

No MeSH data available.


Related in: MedlinePlus

Representative real time tissue responses and resorufin metabolism in the ex vivo bioreactor. The rate of metabolic activity is given by the slope and metabolic activity per unit time is correlated to the time duration of viability experiment in ex vivo conditions similar to those displayed in Figure 5. The media change at each interval is indicated in the figure with arrow mark.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4644552&req=5

fig6: Representative real time tissue responses and resorufin metabolism in the ex vivo bioreactor. The rate of metabolic activity is given by the slope and metabolic activity per unit time is correlated to the time duration of viability experiment in ex vivo conditions similar to those displayed in Figure 5. The media change at each interval is indicated in the figure with arrow mark.

Mentions: To further investigate factors affecting the tissue viability, cell viability indicator resorufin metabolized was analyzed and presented in μM/h (Figure 6). At initial time points, higher resorufin (0.45 μM/h) was metabolized indicating higher tissue viability, but with reduced oxygen levels lower resorufin was metabolized resulting in low tissue viability. In the current ex vivo bioreactor setup, we observed a clear trend as a result of metabolically active cells reducing nonfluorescent resazurin to pink fluorescent resorufin in the presence of mitochondrial dehydrogenase enzymes and over the time interval the metabolic activity is reduced (see Supplementary Figure S4).


Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability.

Mundargi R, Venkataraman D, Kumar S, Mogal V, Ortiz R, Loo J, Venkatraman S, Steele T - Biomed Res Int (2015)

Representative real time tissue responses and resorufin metabolism in the ex vivo bioreactor. The rate of metabolic activity is given by the slope and metabolic activity per unit time is correlated to the time duration of viability experiment in ex vivo conditions similar to those displayed in Figure 5. The media change at each interval is indicated in the figure with arrow mark.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644552&req=5

fig6: Representative real time tissue responses and resorufin metabolism in the ex vivo bioreactor. The rate of metabolic activity is given by the slope and metabolic activity per unit time is correlated to the time duration of viability experiment in ex vivo conditions similar to those displayed in Figure 5. The media change at each interval is indicated in the figure with arrow mark.
Mentions: To further investigate factors affecting the tissue viability, cell viability indicator resorufin metabolized was analyzed and presented in μM/h (Figure 6). At initial time points, higher resorufin (0.45 μM/h) was metabolized indicating higher tissue viability, but with reduced oxygen levels lower resorufin was metabolized resulting in low tissue viability. In the current ex vivo bioreactor setup, we observed a clear trend as a result of metabolically active cells reducing nonfluorescent resazurin to pink fluorescent resorufin in the presence of mitochondrial dehydrogenase enzymes and over the time interval the metabolic activity is reduced (see Supplementary Figure S4).

Bottom Line: The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue.Histological evaluations with hematoxylin and eosin and Masson's trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements.Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.

View Article: PubMed Central - PubMed

Affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798.

ABSTRACT
The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue. Porcine carotid artery as a model tissue was used in the ex vivo bioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure, temperature, and flow. The real time tissue viability was evaluated by monitoring tissue metabolism through a fluorescent indicator "resorufin." Our ex vivo bioreactor allows real time monitoring of tissue responses along with physiological conditions. These ex vivo parameters were vital in determining the tissue viability in sensor-enabled bioreactor and our initial investigations suggest that, porcine tissue viability is considerably affected by high shear forces and low oxygen levels. Histological evaluations with hematoxylin and eosin and Masson's trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements. Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.

No MeSH data available.


Related in: MedlinePlus