Limits...
Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability.

Mundargi R, Venkataraman D, Kumar S, Mogal V, Ortiz R, Loo J, Venkatraman S, Steele T - Biomed Res Int (2015)

Bottom Line: The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue.Histological evaluations with hematoxylin and eosin and Masson's trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements.Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.

View Article: PubMed Central - PubMed

Affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798.

ABSTRACT
The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue. Porcine carotid artery as a model tissue was used in the ex vivo bioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure, temperature, and flow. The real time tissue viability was evaluated by monitoring tissue metabolism through a fluorescent indicator "resorufin." Our ex vivo bioreactor allows real time monitoring of tissue responses along with physiological conditions. These ex vivo parameters were vital in determining the tissue viability in sensor-enabled bioreactor and our initial investigations suggest that, porcine tissue viability is considerably affected by high shear forces and low oxygen levels. Histological evaluations with hematoxylin and eosin and Masson's trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements. Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.

No MeSH data available.


Related in: MedlinePlus

Schematic view of sensor signal assemblage and processing in the ex vivo bioreactor.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4644552&req=5

fig3: Schematic view of sensor signal assemblage and processing in the ex vivo bioreactor.

Mentions: Although sensors dedicated to each parameter are commercially available, our approach offers a more homogenous, easily extensible, and cost-efficient solution. The schematic view of sensor signal assemblage and signal processing is shown in Figure 3. The first stage of the conditioning circuit is specific to each sensor and remains limited to a Wheatstone bridge (4 resistors). In our current biosensor setup, a differential scheme is altered to accommodate a wide range of sensors and the analog input signal goes through antialiasing filters. These signals were amplified and buffered by an instrumentation amplifier (INA116) with an adjustable gain and resulting signals were compatible with the DAQ card inputs in terms of input range, bandwidth to convert acquired signals into digital domain (see Supplementary Figures S1 and S2 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/958170). Finally, raw signals were processed in our MATLAB bioscope. Signal noise was reduced by digital filtering process and conversion from voltage to defined physical quantity was performed and the bioscope allows instant signal monitoring, synchronized recording, and assisted sensor calibration.


Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability.

Mundargi R, Venkataraman D, Kumar S, Mogal V, Ortiz R, Loo J, Venkatraman S, Steele T - Biomed Res Int (2015)

Schematic view of sensor signal assemblage and processing in the ex vivo bioreactor.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644552&req=5

fig3: Schematic view of sensor signal assemblage and processing in the ex vivo bioreactor.
Mentions: Although sensors dedicated to each parameter are commercially available, our approach offers a more homogenous, easily extensible, and cost-efficient solution. The schematic view of sensor signal assemblage and signal processing is shown in Figure 3. The first stage of the conditioning circuit is specific to each sensor and remains limited to a Wheatstone bridge (4 resistors). In our current biosensor setup, a differential scheme is altered to accommodate a wide range of sensors and the analog input signal goes through antialiasing filters. These signals were amplified and buffered by an instrumentation amplifier (INA116) with an adjustable gain and resulting signals were compatible with the DAQ card inputs in terms of input range, bandwidth to convert acquired signals into digital domain (see Supplementary Figures S1 and S2 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/958170). Finally, raw signals were processed in our MATLAB bioscope. Signal noise was reduced by digital filtering process and conversion from voltage to defined physical quantity was performed and the bioscope allows instant signal monitoring, synchronized recording, and assisted sensor calibration.

Bottom Line: The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue.Histological evaluations with hematoxylin and eosin and Masson's trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements.Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.

View Article: PubMed Central - PubMed

Affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798.

ABSTRACT
The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue. Porcine carotid artery as a model tissue was used in the ex vivo bioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure, temperature, and flow. The real time tissue viability was evaluated by monitoring tissue metabolism through a fluorescent indicator "resorufin." Our ex vivo bioreactor allows real time monitoring of tissue responses along with physiological conditions. These ex vivo parameters were vital in determining the tissue viability in sensor-enabled bioreactor and our initial investigations suggest that, porcine tissue viability is considerably affected by high shear forces and low oxygen levels. Histological evaluations with hematoxylin and eosin and Masson's trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements. Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.

No MeSH data available.


Related in: MedlinePlus