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Total Phenolic, Flavonoid, Tomatine, and Tomatidine Contents and Antioxidant and Antimicrobial Activities of Extracts of Tomato Plant.

Silva-Beltrán NP, Ruiz-Cruz S, Cira-Chávez LA, Estrada-Alvarado MI, Ornelas-Paz Jde J, López-Mata MA, Del-Toro-Sánchez CL, Ayala-Zavala JF, Márquez-Ríos E - Int J Anal Chem (2015)

Bottom Line: The leaf extracts from the two varieties showed the highest flavonoids, chlorophyll, carotenoids, and total phenolics contents and the highest antioxidant activity determined by DPPH, ABTS, and ORAC.A positive correlation was observed between the antioxidant capacities of the extracts and the total phenolic, flavonoid, and chlorophyll contents.These results suggest that tomato plant shows well potential as sources of various bioactive compounds, antioxidants, and antimicrobials.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, 85000 Ciudad Obregon, SON, Mexico ; Departamento de Ciencias de la Salud, Universidad de Sonora, Campus Cajeme, Boulevard Bordo Nuevo, Ejido Providencia, 85040 Cajeme, SON, Mexico.

ABSTRACT
The purpose of this study was to evaluate the antioxidant and antimicrobial properties of extracts of different fractions of two tomato plant cultivars. The stems, roots, leaves, and whole-plant fractions were evaluated. Tomatine and tomatidine were identified by HPLC-DAD. The leaf extracts from the two varieties showed the highest flavonoids, chlorophyll, carotenoids, and total phenolics contents and the highest antioxidant activity determined by DPPH, ABTS, and ORAC. A positive correlation was observed between the antioxidant capacities of the extracts and the total phenolic, flavonoid, and chlorophyll contents. The Pitenza variety extracts inhibited the growth of pathogens such as E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, and Listeria ivanovii, yielding inhibition halos of 8.0 to 12.9 mm in diameter and MIC values of 12.5 to 3.125 mg/mL. These results suggest that tomato plant shows well potential as sources of various bioactive compounds, antioxidants, and antimicrobials.

No MeSH data available.


Related in: MedlinePlus

Total phenolic acids (a) and total flavonoids (b) in extracts of tomato plants. The data are mean values ± SD from at least three determinations. The mean values represented by the bars for each type of extract that are indicated with a different letter are significantly different (P ≤ 0.05).
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fig1: Total phenolic acids (a) and total flavonoids (b) in extracts of tomato plants. The data are mean values ± SD from at least three determinations. The mean values represented by the bars for each type of extract that are indicated with a different letter are significantly different (P ≤ 0.05).

Mentions: Plants contain a large variety of phenolic derivatives. These compounds are essential for plant growth and reproduction. In addition, phenolic compounds are natural antioxidants that may occur in all parts of the plant and function as antibiotics and natural pesticides [19]. Figure 1(a) shows the total phenol contents of the examined sections from the Pitenza and Floradade plant tomato varieties. The leaf extracts of the two varieties exhibited the highest phenolic contents with values of 125.5 and 83.35 mg GAE/ge by Pitenza and Floradade, respectively. All of the Pitenza plant sections displayed significantly (P < 0.05) higher phenolic contents compared to the Floradade sections. In contrast, the root extracts displayed the lowest phenolic contents among both the Pitenza and Floradade plant sections (24.5 and 21.98 mg GAE/ge, resp.), showing that roots are not an important reservoir of this type of compound.


Total Phenolic, Flavonoid, Tomatine, and Tomatidine Contents and Antioxidant and Antimicrobial Activities of Extracts of Tomato Plant.

Silva-Beltrán NP, Ruiz-Cruz S, Cira-Chávez LA, Estrada-Alvarado MI, Ornelas-Paz Jde J, López-Mata MA, Del-Toro-Sánchez CL, Ayala-Zavala JF, Márquez-Ríos E - Int J Anal Chem (2015)

Total phenolic acids (a) and total flavonoids (b) in extracts of tomato plants. The data are mean values ± SD from at least three determinations. The mean values represented by the bars for each type of extract that are indicated with a different letter are significantly different (P ≤ 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644540&req=5

fig1: Total phenolic acids (a) and total flavonoids (b) in extracts of tomato plants. The data are mean values ± SD from at least three determinations. The mean values represented by the bars for each type of extract that are indicated with a different letter are significantly different (P ≤ 0.05).
Mentions: Plants contain a large variety of phenolic derivatives. These compounds are essential for plant growth and reproduction. In addition, phenolic compounds are natural antioxidants that may occur in all parts of the plant and function as antibiotics and natural pesticides [19]. Figure 1(a) shows the total phenol contents of the examined sections from the Pitenza and Floradade plant tomato varieties. The leaf extracts of the two varieties exhibited the highest phenolic contents with values of 125.5 and 83.35 mg GAE/ge by Pitenza and Floradade, respectively. All of the Pitenza plant sections displayed significantly (P < 0.05) higher phenolic contents compared to the Floradade sections. In contrast, the root extracts displayed the lowest phenolic contents among both the Pitenza and Floradade plant sections (24.5 and 21.98 mg GAE/ge, resp.), showing that roots are not an important reservoir of this type of compound.

Bottom Line: The leaf extracts from the two varieties showed the highest flavonoids, chlorophyll, carotenoids, and total phenolics contents and the highest antioxidant activity determined by DPPH, ABTS, and ORAC.A positive correlation was observed between the antioxidant capacities of the extracts and the total phenolic, flavonoid, and chlorophyll contents.These results suggest that tomato plant shows well potential as sources of various bioactive compounds, antioxidants, and antimicrobials.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, 85000 Ciudad Obregon, SON, Mexico ; Departamento de Ciencias de la Salud, Universidad de Sonora, Campus Cajeme, Boulevard Bordo Nuevo, Ejido Providencia, 85040 Cajeme, SON, Mexico.

ABSTRACT
The purpose of this study was to evaluate the antioxidant and antimicrobial properties of extracts of different fractions of two tomato plant cultivars. The stems, roots, leaves, and whole-plant fractions were evaluated. Tomatine and tomatidine were identified by HPLC-DAD. The leaf extracts from the two varieties showed the highest flavonoids, chlorophyll, carotenoids, and total phenolics contents and the highest antioxidant activity determined by DPPH, ABTS, and ORAC. A positive correlation was observed between the antioxidant capacities of the extracts and the total phenolic, flavonoid, and chlorophyll contents. The Pitenza variety extracts inhibited the growth of pathogens such as E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, and Listeria ivanovii, yielding inhibition halos of 8.0 to 12.9 mm in diameter and MIC values of 12.5 to 3.125 mg/mL. These results suggest that tomato plant shows well potential as sources of various bioactive compounds, antioxidants, and antimicrobials.

No MeSH data available.


Related in: MedlinePlus