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Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress.

Karami Kheirabad M, Namavar Jahromi B, Tamadon A, Ramezani A, Ahmadloo S, Sabet Sarvestan F, Koohi-Hosseinabadi O - Int J Mol Cell Med (2015)

Bottom Line: Relative expression of MC4R mRNA was determined using real-time PCR.Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05).Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Sciences, Azad University, Gachsaran Branch, Gachsaran, Iran.

ABSTRACT
The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P<0.05). Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.

No MeSH data available.


The effect of chronic stress on the relative expression of MC4R mRNA in the hypothalamus of male rats. Values are expressed as mean± SE. a,b Different superscript letters show significant differences between groups (P< 0.05).
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Figure 1: The effect of chronic stress on the relative expression of MC4R mRNA in the hypothalamus of male rats. Values are expressed as mean± SE. a,b Different superscript letters show significant differences between groups (P< 0.05).

Mentions: There was no significant difference in weights between rats in all groups (Table 2, P>0.05). Relative expression of MC4R mRNA in ARC in the chronic stress group was higher than that of the control rats (P<0.05, Figure 1). Relative expression of MC4R mRNA was not different between the stress, RU486 and stress/RU486 groups (P> 0.05). Further, no difference was observed in relative expression of MC4R mRNA between the control, RU486, and stress/RU486 groups (P> 0.05).


Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress.

Karami Kheirabad M, Namavar Jahromi B, Tamadon A, Ramezani A, Ahmadloo S, Sabet Sarvestan F, Koohi-Hosseinabadi O - Int J Mol Cell Med (2015)

The effect of chronic stress on the relative expression of MC4R mRNA in the hypothalamus of male rats. Values are expressed as mean± SE. a,b Different superscript letters show significant differences between groups (P< 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644530&req=5

Figure 1: The effect of chronic stress on the relative expression of MC4R mRNA in the hypothalamus of male rats. Values are expressed as mean± SE. a,b Different superscript letters show significant differences between groups (P< 0.05).
Mentions: There was no significant difference in weights between rats in all groups (Table 2, P>0.05). Relative expression of MC4R mRNA in ARC in the chronic stress group was higher than that of the control rats (P<0.05, Figure 1). Relative expression of MC4R mRNA was not different between the stress, RU486 and stress/RU486 groups (P> 0.05). Further, no difference was observed in relative expression of MC4R mRNA between the control, RU486, and stress/RU486 groups (P> 0.05).

Bottom Line: Relative expression of MC4R mRNA was determined using real-time PCR.Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05).Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Sciences, Azad University, Gachsaran Branch, Gachsaran, Iran.

ABSTRACT
The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P<0.05). Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.

No MeSH data available.