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Biofilm Formation and Virulence Factors Among Pseudomonas aeruginosa Isolated From Burn Patients.

Ghanbarzadeh Corehtash Z, Khorshidi A, Firoozeh F, Akbari H, Mahmoudi Aznaveh A - Jundishapur J Microbiol (2015)

Bottom Line: Biofilm formation was seen in 92.4% of the isolates.The high rate of MDRPA and its ability to produce biofilm is an alarm for public health.The statistical analysis showed that biofilm production in the MDRPA isolates was significantly higher than that in the non-MDRPA isolates (P < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran.

ABSTRACT

Background: Pseudomonas aeruginosa possesses a variety of virulence factors and infections caused by multidrug-resistant P. aeruginosa (MDRPA) in burn patients are a public health problem.

Objectives: The aim of this study was to determine the antibiotic resistance pattern, the biofilm formation, the prevalence of MDRPA and two virulence genes (nan1 and exoA) among P. aeruginosa isolated from burn patients.

Patients and methods: A total of 144 isolates of P. aeruginosa were collected from burn patient at the Burn Centre of Tehran, Iran, between March 2013 and July 2013. Antibiotic susceptibility test was performed via agar disk diffusion method. The ability of producing biofilm was examined by crystal violet microtiter plate assay and the prevalence of the exoA and nan1 genes among the isolates was determined by polymerase chain reaction (PCR).

Results: A high rate of resistance was seen against ciprofloxacin (93.7%), aztreonam (86.8%), piperacillin (85.4%), ceftazidime (82.6%), amikacin (82%) and imipenem (79.2%). In total, 93.1% of the isolates were characterized as MDRPA. Biofilm formation was seen in 92.4% of the isolates. The prevalence of the exoA and nan1 genes were 75% and 11.8% among the isolates, respectively.

Conclusions: The high rate of MDRPA and its ability to produce biofilm is an alarm for public health. The statistical analysis showed that biofilm production in the MDRPA isolates was significantly higher than that in the non-MDRPA isolates (P < 0.001).

No MeSH data available.


Related in: MedlinePlus

The Relationship Between Biofilm Formation and Multidrug-Resistant Pseudomonas aeruginosa Isolates From Burn Patients
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fig22767: The Relationship Between Biofilm Formation and Multidrug-Resistant Pseudomonas aeruginosa Isolates From Burn Patients

Mentions: Quantitative biofilm determination using the microtiter assay revealed that 133 isolates (92.4%) produced biofilm and the remaining 11 isolates were non-biofilm producers. The relation between biofilm formation and MDRPA is shown in Figure 3. The statistical analysis to examine the link between antibiotic resistance and biofilm formation showed that the biofilm production in MDRPA isolates was significantly higher than that in the non–MDRPA isolates (P < 0.001). Table 3 shows the comparative frequency (as percentages) of virulence factors among the MDRPA and non-MDRPA isolates. The proportion of MDRPA isolates containing two (of three) virulence factors was higher than the proportion of non-MDRPA isolates with two virulence factors (P < 0.01) (Table 3).


Biofilm Formation and Virulence Factors Among Pseudomonas aeruginosa Isolated From Burn Patients.

Ghanbarzadeh Corehtash Z, Khorshidi A, Firoozeh F, Akbari H, Mahmoudi Aznaveh A - Jundishapur J Microbiol (2015)

The Relationship Between Biofilm Formation and Multidrug-Resistant Pseudomonas aeruginosa Isolates From Burn Patients
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644346&req=5

fig22767: The Relationship Between Biofilm Formation and Multidrug-Resistant Pseudomonas aeruginosa Isolates From Burn Patients
Mentions: Quantitative biofilm determination using the microtiter assay revealed that 133 isolates (92.4%) produced biofilm and the remaining 11 isolates were non-biofilm producers. The relation between biofilm formation and MDRPA is shown in Figure 3. The statistical analysis to examine the link between antibiotic resistance and biofilm formation showed that the biofilm production in MDRPA isolates was significantly higher than that in the non–MDRPA isolates (P < 0.001). Table 3 shows the comparative frequency (as percentages) of virulence factors among the MDRPA and non-MDRPA isolates. The proportion of MDRPA isolates containing two (of three) virulence factors was higher than the proportion of non-MDRPA isolates with two virulence factors (P < 0.01) (Table 3).

Bottom Line: Biofilm formation was seen in 92.4% of the isolates.The high rate of MDRPA and its ability to produce biofilm is an alarm for public health.The statistical analysis showed that biofilm production in the MDRPA isolates was significantly higher than that in the non-MDRPA isolates (P < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran.

ABSTRACT

Background: Pseudomonas aeruginosa possesses a variety of virulence factors and infections caused by multidrug-resistant P. aeruginosa (MDRPA) in burn patients are a public health problem.

Objectives: The aim of this study was to determine the antibiotic resistance pattern, the biofilm formation, the prevalence of MDRPA and two virulence genes (nan1 and exoA) among P. aeruginosa isolated from burn patients.

Patients and methods: A total of 144 isolates of P. aeruginosa were collected from burn patient at the Burn Centre of Tehran, Iran, between March 2013 and July 2013. Antibiotic susceptibility test was performed via agar disk diffusion method. The ability of producing biofilm was examined by crystal violet microtiter plate assay and the prevalence of the exoA and nan1 genes among the isolates was determined by polymerase chain reaction (PCR).

Results: A high rate of resistance was seen against ciprofloxacin (93.7%), aztreonam (86.8%), piperacillin (85.4%), ceftazidime (82.6%), amikacin (82%) and imipenem (79.2%). In total, 93.1% of the isolates were characterized as MDRPA. Biofilm formation was seen in 92.4% of the isolates. The prevalence of the exoA and nan1 genes were 75% and 11.8% among the isolates, respectively.

Conclusions: The high rate of MDRPA and its ability to produce biofilm is an alarm for public health. The statistical analysis showed that biofilm production in the MDRPA isolates was significantly higher than that in the non-MDRPA isolates (P < 0.001).

No MeSH data available.


Related in: MedlinePlus