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Complete genome sequence of the thermophilic Acidobacteria, Pyrinomonas methylaliphatogenes type strain K22(T).

Lee KC, Morgan XC, Power JF, Dunfield PF, Huttenhower C, Stott MB - Stand Genomic Sci (2015)

Bottom Line: Its cell membrane is dominated by iso-branching fatty acids, and up to 40 % of its lipid content is membrane-spanning and ether lipids.It is obligately aerobic, thermophilic, moderately acidophilic, and non-spore-forming.The 3,788,560 bp genome of P. methylaliphatogenes K22(T) has a G + C content of 59.36 % and contains 3,189 protein-encoding and 55 non-coding RNA genes.

View Article: PubMed Central - PubMed

Affiliation: GNS Science, Extremophiles Research Group, Taupō, New Zealand.

ABSTRACT
Strain K22(T) is the type species of the recently- described genus Pyrinomonas, in subdivision 4 of the phylum Acidobacteria (Int J Syst Evol Micr. 2014; 64(1):220-7). It was isolated from geothermally-heated soil from Mt. Ngauruhoe, New Zealand, using low-nutrient medium. P. methylaliphatogenes K22(T) has a chemoheterotrophic metabolism; it can hydrolyze a limited range of simple carbohydrates and polypeptides. Its cell membrane is dominated by iso-branching fatty acids, and up to 40 % of its lipid content is membrane-spanning and ether lipids. It is obligately aerobic, thermophilic, moderately acidophilic, and non-spore-forming. The 3,788,560 bp genome of P. methylaliphatogenes K22(T) has a G + C content of 59.36 % and contains 3,189 protein-encoding and 55 non-coding RNA genes. Genomic analysis was consistent with nutritional requirements; in particular, the identified transporter classes reflect the oligotrophic nature of this strain.

No MeSH data available.


Related in: MedlinePlus

Graphical map of the genome of P. methylaliphatogenes K22T showing the eight largest scaffolds. From bottom to the top of each scaffold: Genes on forward strand (color by COG categories as denoted by the IMG platform), genes on the reverse strand (color by COG categories), RNA genes (tRNAs – green, sRNAs – red, other RNAs – black), GC content, and GC skew
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Fig3: Graphical map of the genome of P. methylaliphatogenes K22T showing the eight largest scaffolds. From bottom to the top of each scaffold: Genes on forward strand (color by COG categories as denoted by the IMG platform), genes on the reverse strand (color by COG categories), RNA genes (tRNAs – green, sRNAs – red, other RNAs – black), GC content, and GC skew

Mentions: The QD assembly of the genome consists of 16 scaffolds totaling 3,788,560 bp in length (59.36 % GC content). Of the 3,244 genes predicted, 3,189 were protein-coding genes, and 55 were non-coding RNA genes. A majority (79.0 %) of genes were assigned putative functions, and the remainder were annotated as hypothetical proteins. The properties and the statistics of the P. methylaliphatogenes K22T genome and the distribution of genes into COG functional categories are presented in Table 3, Table 4, and Fig. 3.Table 3


Complete genome sequence of the thermophilic Acidobacteria, Pyrinomonas methylaliphatogenes type strain K22(T).

Lee KC, Morgan XC, Power JF, Dunfield PF, Huttenhower C, Stott MB - Stand Genomic Sci (2015)

Graphical map of the genome of P. methylaliphatogenes K22T showing the eight largest scaffolds. From bottom to the top of each scaffold: Genes on forward strand (color by COG categories as denoted by the IMG platform), genes on the reverse strand (color by COG categories), RNA genes (tRNAs – green, sRNAs – red, other RNAs – black), GC content, and GC skew
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4644332&req=5

Fig3: Graphical map of the genome of P. methylaliphatogenes K22T showing the eight largest scaffolds. From bottom to the top of each scaffold: Genes on forward strand (color by COG categories as denoted by the IMG platform), genes on the reverse strand (color by COG categories), RNA genes (tRNAs – green, sRNAs – red, other RNAs – black), GC content, and GC skew
Mentions: The QD assembly of the genome consists of 16 scaffolds totaling 3,788,560 bp in length (59.36 % GC content). Of the 3,244 genes predicted, 3,189 were protein-coding genes, and 55 were non-coding RNA genes. A majority (79.0 %) of genes were assigned putative functions, and the remainder were annotated as hypothetical proteins. The properties and the statistics of the P. methylaliphatogenes K22T genome and the distribution of genes into COG functional categories are presented in Table 3, Table 4, and Fig. 3.Table 3

Bottom Line: Its cell membrane is dominated by iso-branching fatty acids, and up to 40 % of its lipid content is membrane-spanning and ether lipids.It is obligately aerobic, thermophilic, moderately acidophilic, and non-spore-forming.The 3,788,560 bp genome of P. methylaliphatogenes K22(T) has a G + C content of 59.36 % and contains 3,189 protein-encoding and 55 non-coding RNA genes.

View Article: PubMed Central - PubMed

Affiliation: GNS Science, Extremophiles Research Group, Taupō, New Zealand.

ABSTRACT
Strain K22(T) is the type species of the recently- described genus Pyrinomonas, in subdivision 4 of the phylum Acidobacteria (Int J Syst Evol Micr. 2014; 64(1):220-7). It was isolated from geothermally-heated soil from Mt. Ngauruhoe, New Zealand, using low-nutrient medium. P. methylaliphatogenes K22(T) has a chemoheterotrophic metabolism; it can hydrolyze a limited range of simple carbohydrates and polypeptides. Its cell membrane is dominated by iso-branching fatty acids, and up to 40 % of its lipid content is membrane-spanning and ether lipids. It is obligately aerobic, thermophilic, moderately acidophilic, and non-spore-forming. The 3,788,560 bp genome of P. methylaliphatogenes K22(T) has a G + C content of 59.36 % and contains 3,189 protein-encoding and 55 non-coding RNA genes. Genomic analysis was consistent with nutritional requirements; in particular, the identified transporter classes reflect the oligotrophic nature of this strain.

No MeSH data available.


Related in: MedlinePlus