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The Role of NMDARs Ligands on Antinociceptive Effects of Pregabalin in the Tail Flick Test.

Meymandi MS, Keyhanfar F, Yazdanpanah O, Heravi G - Anesth Pain Med (2015)

Bottom Line: In NMDA pretreated groups, %MPE30 unlike %MPE75 decreased significantly compared to those of pregabalin.MK801 delayed the latency times in pretreated groups, but %MPE30 and %MPE75 did not change significantly compared to pregabalin alone.Our findings support the role of NMDARs in pregabalin antinociception, because the NMDAR agonist, unlike the antagonist, decreased the antinociceptive effect of pregabalin, even if tail flick is not an adequate pain assessment method in this regard.

View Article: PubMed Central - PubMed

Affiliation: Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

ABSTRACT

Background: Pregabalin as a new anticonvulsant has been used in different pain treatments.

Objectives: The aim of this study was to investigate the role of N-methyl-D-aspartate (NMDA) ligands in antinociceptive effect of pregabalin in mice using tail flick.

Materials and methods: NMDA (15 and 30 mg/kg) as an agonist or MK801 (0.02 and 0.05 mg/kg) as an antagonist were injected intraperitoneally either alone or 15 minutes before antinociceptive dose of pregabalin (100 mg/kg). Then the latency times and %MPE were measured in the tail flick assay during 75 minutes.

Results: NMDA and MK801 had no effects alone. NMDA pretreatment significantly decreased the latency times of pregabalin till 75(th) minutes. In NMDA pretreated groups, %MPE30 unlike %MPE75 decreased significantly compared to those of pregabalin. MK801 delayed the latency times in pretreated groups, but %MPE30 and %MPE75 did not change significantly compared to pregabalin alone.

Conclusions: Our findings support the role of NMDARs in pregabalin antinociception, because the NMDAR agonist, unlike the antagonist, decreased the antinociceptive effect of pregabalin, even if tail flick is not an adequate pain assessment method in this regard.

No MeSH data available.


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Mentions: In our previous studies, the dose of pregabalin that produced approximately 30% antinociception in tail flick test was 100 mg/kg/ip (11, 12). The control group received normal saline (control) and the pregabalin group (pg) received doses of 100 mg/kg. Other groups received NMDA at doses of 30 or 15 mg/kg (NMDA30 and NMDA15) and MK801 at doses of 0.02 or 0.05 mg/kg (MK.02 and MK.05). The pretreated groups received 30 or 15 mg/kg of NMDA before pregabalin (pg + NMDA30), (pg + NMDA15) or 0.02 or 0.05 mg/kg of MK801 15 minutes before pregabalin (pg + MK.02), (pg + MK.05) (Figure 1).


The Role of NMDARs Ligands on Antinociceptive Effects of Pregabalin in the Tail Flick Test.

Meymandi MS, Keyhanfar F, Yazdanpanah O, Heravi G - Anesth Pain Med (2015)

Procedure Grouping
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644310&req=5

fig23322: Procedure Grouping
Mentions: In our previous studies, the dose of pregabalin that produced approximately 30% antinociception in tail flick test was 100 mg/kg/ip (11, 12). The control group received normal saline (control) and the pregabalin group (pg) received doses of 100 mg/kg. Other groups received NMDA at doses of 30 or 15 mg/kg (NMDA30 and NMDA15) and MK801 at doses of 0.02 or 0.05 mg/kg (MK.02 and MK.05). The pretreated groups received 30 or 15 mg/kg of NMDA before pregabalin (pg + NMDA30), (pg + NMDA15) or 0.02 or 0.05 mg/kg of MK801 15 minutes before pregabalin (pg + MK.02), (pg + MK.05) (Figure 1).

Bottom Line: In NMDA pretreated groups, %MPE30 unlike %MPE75 decreased significantly compared to those of pregabalin.MK801 delayed the latency times in pretreated groups, but %MPE30 and %MPE75 did not change significantly compared to pregabalin alone.Our findings support the role of NMDARs in pregabalin antinociception, because the NMDAR agonist, unlike the antagonist, decreased the antinociceptive effect of pregabalin, even if tail flick is not an adequate pain assessment method in this regard.

View Article: PubMed Central - PubMed

Affiliation: Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

ABSTRACT

Background: Pregabalin as a new anticonvulsant has been used in different pain treatments.

Objectives: The aim of this study was to investigate the role of N-methyl-D-aspartate (NMDA) ligands in antinociceptive effect of pregabalin in mice using tail flick.

Materials and methods: NMDA (15 and 30 mg/kg) as an agonist or MK801 (0.02 and 0.05 mg/kg) as an antagonist were injected intraperitoneally either alone or 15 minutes before antinociceptive dose of pregabalin (100 mg/kg). Then the latency times and %MPE were measured in the tail flick assay during 75 minutes.

Results: NMDA and MK801 had no effects alone. NMDA pretreatment significantly decreased the latency times of pregabalin till 75(th) minutes. In NMDA pretreated groups, %MPE30 unlike %MPE75 decreased significantly compared to those of pregabalin. MK801 delayed the latency times in pretreated groups, but %MPE30 and %MPE75 did not change significantly compared to pregabalin alone.

Conclusions: Our findings support the role of NMDARs in pregabalin antinociception, because the NMDAR agonist, unlike the antagonist, decreased the antinociceptive effect of pregabalin, even if tail flick is not an adequate pain assessment method in this regard.

No MeSH data available.